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  • Immunohistochemistry  (1)
  • Polymer and Materials Science  (1)
  • Tumor marker  (1)
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  • 1
    ISSN: 1432-2307
    Keywords: Endothelial cells ; Monocyte ; Liver ; Immunohistochemistry ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary During studies of the antigenic and functional properties of hepatic sinusoidal lining cells in situ, we found that only the sinusoidal endothelial cells share antigens with a peripheral blood macrophage subset capable of presenting soluble antigens and triggering autologous mixed lymphocyte reactions. They were HLA-DR+, OKM1−, OKM5+. Vascular endothelial cells in the portal areas and central veins were HLA-DR+, OKM1− and OKM5−. The sinusoidal endothelial cells also expressed an antigen found on helper/inducer (OKT4 and Leu3 a) T lymphocytes. Thus, the present study suggests that endothelial cells in different anatomic compartments in the liver heterogenously express surface antigens associated with monocytes, macrophages and T lymphocytes and possess distinct immunological functions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 18 (1990), S. 175-180 
    ISSN: 1434-0879
    Keywords: Seminoma ; Enolase ; Isozyme ; Enzyme immunoassay ; Tumor marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We determined concentrations of α and γ-enolases in normal testis and in seminoma tissues by enzyme immunoassay. Concentrations of α-enolase were 4,170±2,040 ng/mg protein in normal testis (n = 8) and 8,140±4,480 ng/mg protein in seminoma (n = 8). Concentrations of γ-enolase in seminoma (460±571 ng/mg protein) were significantly higher than those of normal testis (59±15 ng/mg protein). Immunohistochemistry showed positive tumor cells for γ-enolase in 6 of 8 seminoma cases (75%). Serum γ-enolase levels were elevated (〉 6.0 ng/ml) in 9 of 12 patients (75%) with seminoma: 60% of stage I, and 100% of stages II and III. In 10 patients treated by surgical excision and chemotherapy, serum γ-enolase was significantly reduced after the treatment. These findings indicate that elevated serum γ-enolase is derived from enhanced γ-enolase in seminoma tissues, and that serum γ-enolase could be a useful biomarker for staging and monitoring clinical course in patients with seminoma.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 11 (1988), S. 94-102 
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The possibility of quantitative AES analysis using the whole EN(E) spectrum was examined for Au—Cu alloy samples. The EN(E) spectrum was measured under ϕ = 0° (normal) incidence of 10 keV electrons for the Au-44 at% Cu sample, and under ϕ = 0°, 30°, and 45° incidence of 10 keV primary electrons for the Au-20 at% Cu sample. Those spectra were compared with spectra which were obtained by linear combination of pure Au and Cu spectra measured under the same experimental conditions.Both measured and synthesized spectra coincided very well in the energy region lower than 1000 eV, except the region of slow secondary electrons. The results indicate that energy distribution of backscattered electrons of the Au—Cu alloy can be estimated well by linear combinations of pure sample spectra. They also indicate that, by comparison of the shape of the measured EN(E) spectrum with the synthesized one, the surface composition of the Au—Cu alloy sample can be estimated with reasonable accuracy (several % error).
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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