ISSN:
1434-0879
Keywords:
Seminoma
;
Enolase
;
Isozyme
;
Enzyme immunoassay
;
Tumor marker
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary We determined concentrations of α and γ-enolases in normal testis and in seminoma tissues by enzyme immunoassay. Concentrations of α-enolase were 4,170±2,040 ng/mg protein in normal testis (n = 8) and 8,140±4,480 ng/mg protein in seminoma (n = 8). Concentrations of γ-enolase in seminoma (460±571 ng/mg protein) were significantly higher than those of normal testis (59±15 ng/mg protein). Immunohistochemistry showed positive tumor cells for γ-enolase in 6 of 8 seminoma cases (75%). Serum γ-enolase levels were elevated (〉 6.0 ng/ml) in 9 of 12 patients (75%) with seminoma: 60% of stage I, and 100% of stages II and III. In 10 patients treated by surgical excision and chemotherapy, serum γ-enolase was significantly reduced after the treatment. These findings indicate that elevated serum γ-enolase is derived from enhanced γ-enolase in seminoma tissues, and that serum γ-enolase could be a useful biomarker for staging and monitoring clinical course in patients with seminoma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00295843
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