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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 69 (1984), S. 205-210 
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; Rye ; Barley ; Leaf peroxidase ; Isozymes ; Isoelectric focusing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eight leaf peroxidase isozymes were distinguished by IEF in ‘Chinese Spring’. Two genes which control the production of three of these isozymes were located on chromosome arms 1BS and 1DS by nullisomic analysis. These loci probably form part of a homoeoallelic series and have been designated Per-B1 and Per-D1 respectively. Analysis of chromosome 1B short arm terminal deletion stocks indicated that the Per-B1 locus is located between the nucleolar organiser region and another isozyme marker, Hk-B1. Two variant leaf peroxidase phenotypes were distinguished in a small sample of hexaploid wheat varieties. Analysis of wheat-alien addition and substitution lines identified homoeologous loci in rye (Per-R1) and barley (Per-H1).
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 72 (1986), S. 219-225 
    ISSN: 1432-2242
    Keywords: Esterase isozymes ; Isoelectric focusing ; Wheat ; Rye ; Barley ; Aegilops ; Agropyron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison of EST-5 grain esterase phenotypes from wheat-alien amphiploid, addition and substitution genotypes, resolved by flat-bed isoelectric focusing identified homoeologous Est-5 loci on chromosome 3H of Hordeum vulgare, 3Hch of H. chilense, 3Sb of Aegilops bicornis, 3S1 of Ae. sharonensis and Ae. longissima and 6R of Secale cereale and 6Rm of S. montanum. The Est-5 genes in alien species provide evidence for chromosome homoeology with wheat.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Chromosome pairing ; Genomic in situ hybridization ; Meiosis ; Rye ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in-situ hybridization (GISH) was used to determine the amount of wheat-rye chromosome pairing in wheat (Triticum aestivum) x rye (Secale cereale) hybrids having chromosome 5B present, absent, or replaced by an extra dose of chromosome 5D. The levels of overall chromosome pairing were similar to those reported earlier but the levels of wheat-rye pairing were higher than earlier determinations using C-banding. Significant differences in chromosome pairing were found between the three genotypes studied. Both of the chromosome-5B-deficient hybrid genotypes showed much higher pairing than the euploid wheat hybrid. However, the 5B-deficient hybrid carrying an extra chromosome 5D had significantly less wheat-rye pairing than the simple 5B-deficient genotype, indicating the presence of a suppressing factor on chromosome 5D. Non-homologous/non-homoeologous chromosome pairing was observed in all three hybrid genotypes. The value of GISH for assessing the level of wheat-alien chromosome pairing in wheat/alien hybrids and the effectiveness of wheat genotypes that affect homoeologous chromosome pairing is demonstrated.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 145-147 
    ISSN: 1432-2242
    Keywords: Diploid wheat ; Nucleolus organiser ; In situ hybridisation ; Telocentric markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The two nucleolus organiser chromosomes of diploid wheat are identified as 1A and 5A by the combination of in situ hybridisation and cytological markers.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 58 (1980), S. 97-100 
    ISSN: 1432-2242
    Keywords: Nucleolus organizers ; In situ hybridisation ; Diploid wheats ; Ribosomal RNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Labelled RNA, transcribed in vitro from wheat ribosomal DNA cloned in a bacterial plasmid, has been hybridised to metaphase chromosomes of five diploid wheats. Autoradiography of the chromosomes has provided unequivocal evidence that these genotypes possess two pairs of nucleolus organizer chromosomes. The diploid wheat accessions used possess widely differing numbers of ribosomal RNA genes.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2242
    Keywords: Triticum timopheevi ; Chromosomes ; C-banding ; In situ hybridisation ; Heterochromatin ; Translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosomes of the tetraploid wheats Triticum timopheevi (Genome AAGG) and T. araraticum (Genome AAGG) were C-banded at mitosis. The identity of the banded and unbanded chromosomes was then established by firstly making comparisons with the hexaploid species T. zhukovskyi which has the genome formula AAAAGG. Secondly, the meiotic pairing in F1 hybrids between T. timopheevi and diploid wheats was examined by means of C-banding. The results showed that the banded chromosomes belonged to the G genome, while the unbanded chromosomes belonged to the A genome. Only one of the two pairs of satellited chromosomes had strong heterochromatic bands. The relationship between the genomes of T. timopheevi and T. dicoccum (Genome AABB) was then assessed at meiosis in hybrids between these species, using the techniques of C-banding and in situ hybridisation of a cloned ribosomal RNA gene probe. It was concluded that there were differences both in the amount and distribution of heterochromatin and also translocation differences between the species.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 61 (1982), S. 285-288 
    ISSN: 1432-2242
    Keywords: Nucleolar organisers ; In situ hybridisation ; Tetraploid and hexaploid wheats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of in situ hybridisation of cloned ribosomal DNA has been used to establish the numbers of nucleolar organising sites in a range of tetraploid and hexaploid wheats.
    Type of Medium: Electronic Resource
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