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Some ultrastructural effects of testosterone and insulin on the ventral prostate of rats in organ culture (1977)

Ichihara, Ichiro

Springer

Cell & tissue research 181 (1977), S. 327-337

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ISSN: 1432-0878

Keywords: Prostate ; Tissue culture ; Testosterone ; Insulin ; Ultrastructure

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The fine structure of the secretory epithelial cells of rat's ventral prostate has been studied following organ culture. Culturing with either testosterone or insulin alone, and with the two hormones combined, were carried out to investigate how insulin modifies the action of testosterone on the maintenance of cellular integrity. After 4 days in hormone-free culture, the secretory epithelial cells showed signs of cellular atrophy and regression, involving loss of the apical microvilli, absence of the apical secretory vacuoles, atrophy of the Golgi apparatus, decrease in rough endoplasmic reticulum and the appearance of autophagic vacuoles. The presence in the medium of either testosterone or insulin alone, or combined, prevented cellular atrophy and regression. The best maintenance of cellular integrity was obtained in a culture containing both hormones. The effects of insulin was approximately equivalent to those of testosterone in the maintenance of cellular integrity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00223108

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Cholesterol changes in developing testicular interstitial cells of the mouse: Histochemical and biochemical study (1969)

Ichihara, Ichiro

New York, NY [u.a.] : Wiley-Blackwell

The @Anatomical Record 163 (1969), S. 595-601

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ISSN: 0003-276X

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: In combination with biochemical determination of cholesterol in testes, cholesterol was studied histochemically in the testicular interstitial cells of dd strain mice from 5 to 63 days.Cholesterol is found in interstitial cells at five days and cholesterol positive interstitial cells increase significantly from 7% at 5 days to 47% at 21 days (17% at 7 days, 21% at 10 days and 31% at 14 days) and thereafter gradually to 58% at 63 days (50% at 28 days, 54% at 35 days, 56% at 42 days, 58% at 49 days and 57% at 56 days).The cholesterol concentration of testes (μg of cholesterol per mg testicular weight) remains at low values before 42 days (1.33 at 5 days, 2.12 at 7 days, 1.89 at 10 days, 2.18 at 14 days, 2.16 at 21 days, 1.63 at 28 days and 2.09 at 35 days) but it increases rapidly at days 42-49 (2.21 at 42 days and 5.16 at 49 days) and then it is almost unchanged (5.47 at 56 days and 5.18 at 63 days).From these results it is suggested that (1) at five days testicular steroid could be elaborated and (2) the capacity of interstitial cells to produce testicular steroid elevates actively from 42 to 49 days.

Additional Material: 1 Ill.