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  • Interphase cytogenetics  (1)
  • PET and acrylic coating  (1)
  • PP  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 778-786 
    Details
    ISSN: 1432-2242
    Keywords: Genomic probing ; In situ hybridization ; Interphase cytogenetics ; Physical mapping ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genomic in situ hybridization was used to identify alien chromatin in chromosome spreads of wheat, Triticum aestivum L., lines incorporating chromosomes from Leymus multicaulis (Kar. and Kir.) Tzvelev and Thinopyrum bessarabicum (Savul. and Rayss) Löve, and chromosome arms from Hordeum chilense Roem. and Schult, H. vulgare L. and Secale cereale L. Total genomic DNA from the introgressed alien species was used as a probe, together with excess amounts of unlabelled blocking DNA from wheat, for DNA:DNA in-situ hybridization. The method labelled the alien chromatin yellow-green, while the wheat chromosomes showed only the orange-red fluorescence of the DNA counterstain. Nuclei were screened from seedling root-tips (including those from half-grains) and anther wall tissue. The genomic probing method identified alien chromosomes and chromosome arms and allowed counting in nuclei at all stages of the cell cycle, so complete metaphases were not needed. At prophase or interphase, two labelled domains were visible in most nuclei from disomic lines, while only one labelled domain was visible in monosomic lines. At metaphase, direct visualization of the morphology of the alien chromosome or chromosome segment was possible and allowed identification of the relationship of the alien chromatin to the wheat chromosomes. The genomic in-situ hybridization method is fast, sensitive, accurate and informative. Hence it is likely to be of great value for both cytogenetic analysis and in plant breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227384
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In situ and Real-Time Monitoring of Plasma-Induced Etching of PET and Acrylic Films (1999)
    Springer
    Plasmas and polymers 4 (1999), S. 247-258 
    Details
    ISSN: 1572-8978
    Keywords: Plasma ; polymer etching ; in situ detection ; PET and acrylic coating
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Residual gas analysis (RGA) and optical emission spectroscopy have been evaluated as potential in situ techniques for the detection of plasma-induced polymer surface etching. The detection is based on the measurement of CO and CO2 species formed in the gas phase following oxidation of the etching fragments released from the polymer surface. Experiments were performed on poly(ethylene terephthalate) and UV-cured acrylic (tripropylene glycol diacrylate) films exposed to O2 RF (13.56 MHz) plasmas. A linear correlation is obtained between the formation of CO and the polymer etching rate over the entire experimental range, but discrepancies appear for the formation of CO2 at high treatment powers (etching rate 〉 1.0 μg/min.cm2). This behavior is attributed to a deficit of oxidizing agents relative to the generation of etching fragments. The results suggest that both RGA and optical emission spectroscopy can be used to monitor in situ and in real-time the etching of polymer surfaces during plasma treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1021875310148
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Polymer treatment in the flowing afterglow of an oxygen microwave discharge: Active species profile concentrations and kinetics of the functionalization (1995)
    Springer
    Plasma chemistry and plasma processing 15 (1995), S. 173-198 
    Details
    ISSN: 1572-8986
    Keywords: Singlet molecular oxygen ; atomic oxygen ; flowing afterglow ; downstream reactor ; microwave discharge ; PP ; PF ; PC ; hexatriacontane ; octadecyloctadecanoate ; functionalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract The surface treatment of different polymers and their corresponding model surfaces in the flowing afterglow of an oxygen microwave plasma is investigated. The concentration profiles of tire long-lived species issued from the plasma are measured and calculated in the downstream area The influence of atomic and singlet molecular oxygen in the behavior of different polymers is investigated. It appears that the evolution of the surface energy can be explained by an initiation of the functionalization by the oxygen atoms impinging upon the surface followed by reaction of the radicals formed with molecular oxygen. The concentration of functions at the sureface is limited due to their destruction by reaction with oxygen atoms. Furthermore, the functionalization level is higher in the /lowing afterglow than in the plasma, without any significant degradation of the polymer surface. Therefore, the treatment in the flowing afterglow is more efficient to increase suface energy in particular, for polymers which undergo high backbone chain scission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01459695
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    Paper (German National Licenses)
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