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  • 1
    ISSN: 1432-2013
    Keywords: Key words Inward-rectifier ; Polyamine block ; Spermine ; Heterooligomers ; IRK1 ; BIR10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Activities of strong inward-rectifier K+ channels composed of Kir2.1(84 M), Kir2.1(84T) and Kir4.1 subunits and weak inward-rectifier K+ channels composed of Kir4.1(E158N) subunits were measured from giant inside-out patches of Xenopus laevis oocytes. The conductance/voltage (g/V) relationship for block by intracellular spermine (SPM) was biphasic for both Kir2.1 channel types while it was monophasic for both Kir4.1 channel types. The release of blocking Mg2+ ions was slow for Kir2.1(84T) but virtually instantaneous for Kir2.1(84M) and both Kir4.1 channel types. Coexpression of Kir2.1(84T) and Kir4.1(E158N) resulted in heterooligomeric channels which were strongly rectifying, with a g/V relationship for SPM-evoked block that was significantly different from that of either parental homooligomeric channel type. Block by intracellular Mg2+ was markedly stronger than that for Kir4.1(E158N) channels, while release of the block was almost instantaneous, similar to that for Kir4.1(E158N) channels. This suggests preferential formation of a particular heterooligomer such as was recently proposed for subunits within the Kir3.0 family.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Pharmacy world & science 21 (1999), S. 158-167 
    ISSN: 1573-739X
    Keywords: Control Groups ; Intervals ; Interventions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The aim of this overview was to identify interventions that change doctor prescribing behaviour and to derive conclusions for practice and further research. Relevant studies (indicating prescribing as a behaviour change) were located from a database of studies maintained by the Cochrane Collaboration on Effective Professional Practice. This register is kept up to date by searching the following databases for reports of relevant research: DHSS‐DATA; EMBASE; MEDLINE; SIGLE; Resource Database in Continuing Medical Education (1975‐1994), along with bibliographies of related topics, hand searching of key journals and personal contact with content area experts. Randomised controlled trials and non‐equivalent group designs with pre‐ and post‐intervention measures were included. Outcome measures were those used by the study authors. For each study we determined whether these were positive, negative or inconclusive. Positive studies (+) were those that demonstrated a statistically significant change in the majority of outcomes measured at level of p ≤0.05 between the intervention and control groups. Negative studies (‐) showed a significant change in the opposite direction and inconclusive studies (≈) showed no significant change compared to control or no overall positive findings. We identified 79eligible studies which described 96 separate interventions to change prescribing behaviour. Of these interventions, 49 (51%, 41%‐61%) showed a positive significant change compared to the control group but interpretation of specific interventions is limited due to wide and overlapping confidence intervals.
    Type of Medium: Electronic Resource
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