Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Key words: Histamine — Histamine receptors — Inflammation — Neutrophils — Signal transduction  (1)
  • Laparoscopically assisted sigmoid resection  (1)
  • 1
    ISSN: 1420-908X
    Keywords: Key words: Histamine — Histamine receptors — Inflammation — Neutrophils — Signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective and Design: In order to understand the underlying mechanism of histamine stimulated inflammatory responses, histamine receptor subtypes and signal transduction pathways by which histamine mediates the stimulation of neutrophil adhesion to endothelial cells has been studied in vitro.¶Material: Human neutrophils and human umbilical vein endothelial cells.¶Treatment: Confluent human endothelial cell layer were incubated with histamine (1 mM), H1, H2 or H3 receptor agonists: fluorophenylhistamine (10 μM), amthamine (10 μm), methylhistamine (10 μM), respectively. Ten minutes prior to histamine (1 mM) stimulation H1, H2 or H3 receptor antagonists (dimethindene, 100 μM; famotidine, 100 μM, thioperamid 100 μM, respectively) were added. Histamine stimulated signal transduction pathways were inhibited by adding phospholipase C inhibitor 2-nitro-4-carboxyphenyl N,N-diphenylcarbamat (200 μM), adenylate cyclase inhibitor 9-(2 tetrahydrofuryl)adenine (80 μM), nitric oxide synthase isozymes inhibitor S-ethylisothiourea (1 μM) or guanylate cyclase inhibitor (LY 83583; 10 μM). Neutrophil adhesion was monitored at 30, 60, 90, 120, 150, 180 and 210 min.¶Methods: Neutrophil adhesion to endothelial cells was quantified by analysing alkaline phosphatase activity.¶Results: Histamine stimulation of endothelial cells resulted in a biphasic time and concentration dependent pattern of neutrophil adhesion. This pattern of neutrophil adhesion was mimicked by stimulation of endothelial cells with H1 or H2 agonists. Stimulation of endothelial cells with an H3 agonist had no effect on neutrophil binding. Inhibition of phospholipase C (PLC), nitric oxide synthase isozymes (NOS) or guanylate cyclase (GC) resulted in a significant decrease of neutrophil binding to histamine or agonist stimulated endothelial cells. An increase of neutrophil binding to unstimulated or to agonist stimulated endothelial cells was observed during inhibition of adenylate cyclase.¶Conclusions: Our results suggest that histamine stimulated neutrophil adhesion is due to H1 and H2 receptor mediated activation of PLC, NOS and GC. Increase of cAMP concentration seems to mediate an inhibitory effect on PMN adhesion to endothelial cells
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International journal of colorectal disease 13 (1998), S. 43-47 
    ISSN: 1432-1262
    Keywords: Key words Diverticular disease ; Laparoscopically assisted sigmoid resection ; Open sigmoid resection ; Recovery ; Morbidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le but de cette étude est d'évaluer le devenir des patients ayant subi une sigmoïdectomie laparoscopique pour maladie diverticulaire. Vingt-neuf patients consécutifs ont été traités chirurgicalement pour une diverticulite colique. Vingt-sept des cas ont pu être opérés par laparoscopie, L'étude des dossiers cliniques d'un groupe-contrôle de 34 patients subissant une résection ouverte a servi de comparaison. Le taux de conversion est de 7,5%. Par laparoscopie, la durée de l'intervention chirurgicale a été plus longue (165 vs 121 minutes, P〈0,05), les pertes sanguines (182 vs 352 ml, P〈0,05) et les transfusions sanguines moindres (0 vs 16%). L'incidence des complications suivant la laparoscopie a été inférieure (2 fuites anastomotiques, 2 infections de plaie) en comparaison à la chirurgie conventionnelle. La convalescence du groupe laparoscopique a été plus rapide et la durée d'hospitalisation réduite (7,9 vs 14,3 jours, P〈0,05). Dans le groupe laparascopique, les douleurs au repos et aux mouvements ont été inférieures. Let coût du geste chirurgical laparoscopique a été inférieur en comparaison à la résection conventionelle (7185 vs 8975 DM). Dans cette série, la résection sigmoïdienne par laparoscopie pour maladie diverticulaire est sûre et plus rapide, la durée de guérison est plus rapide, la durée d'hospitalisation plus brève et les patients ont présenté moins de douleurs dans le groupe laparoscopique en comparaison avec la chirurgie conventionelle.
    Notes: Abstract This study evaluated outcome in patients undergoing laparoscopically assisted sigmoid resection for diverticular disease. A total of 29 consecutive patients were treated surgically for colonic diverticulitis; in 27 of these laparoscopy was performed. The review of medical records from a control group of 34 patients undergoing open resection were used for comparison. The conversion rate was 7.5%. Using the laparoscopic technique the duration of surgery was longer (165 vs. 121 min, P〈0.05), blood loss less (182 vs. 352 ml, P〈0.05), and subsequent blood transfusion less (0 vs. 61%). The incidence of complications following laparoscopic resection was lower (two anastomotic leakages, two wound infections) than in the conventional group. Convalescence in the laparoscopic group was more rapid and hospital stay shorter (7.9 vs. 14.3 days, P〈0.05). In the laparoscopic group patients expressed less pain at rest and in motion. The cost of the laparoscopically assisted procedure was less than that of conventional resection (7185 vs. 8975 DM). In this series laparoscopically assisted sigmoid resection for diverticulitis proved safe. Recovery was faster, hospital stay was shorter, and patients expressed less pain than in conventional open surgery.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...