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  • 1
    Electronic Resource
    Electronic Resource
    Observations of pollen tube growth in Nicotiana alata and their implications for the mechanism of self-incompatibility (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 27-35 
    Details
    ISSN: 1432-2145
    Keywords: Key words Nicotiana alata ; Pollen tube growth ; Stylar graft ; Self-incompatibility ; Cytotoxic model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Style squashes and stylar grafts were used to examine the growth of Nicotiana alata pollen tubes in self-compatible and self-incompatible styles. Compatible tubes typically showed a uniform layer of callose deposition in the walls and in small plugs spaced at regular intervals within the tube. Incompatible tubes were characterised by the variability of callose deposition in the walls and by larger, closer and more irregularly spaced plugs. There was no difference in the growth rate of compatible and incompatible tubes during growth through the stigma, but within the style most compatible tubes grew 20–25 mm day-1 (maximum 30 mm day–1), whereas incompatible tubes grew 1.0–1.5 mm day-1 (maximum 5 mm day–1). Many incompatible tubes continued to grow until flowers senesced, and only a small proportion died as a consequence of tip bursting. Grafting compatibly pollinated styles onto incompatible styles showed that the incompatible reaction could occur in pollen tubes between 2 and 50 mm long, and that inhibition of pollen tube growth occurred in both the upper and lower parts of the transmitting tract. Grafting incompatibly pollinated styles onto compatible styles showed that the incompatible reaction was fully reversible in at least a proportion of the pollen tubes. The findings are not consistent with the cytotoxic model of inhibition of self-pollen tubes in solanaceous plants, which assumes that the incompatible response results from the degradation of a finite amount of rRNA present in the pollen tube. However, if pollen tubes do in fact synthesise rRNA, the findings become consistent with this model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050064
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Growth inhibition of suspension cultured plant cells by ribonucleases (1995)
    Springer
    Journal of plant research 108 (1995), S. 305-312 
    Details
    ISSN: 1618-0860
    Keywords: Nicotiana alata ; Nicotiana plumbaginifolia ; Pollen ; Ribonuclease ; Self-incompatibility ; Toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracellular, stylar RNases (S-RNases) are produced by self-incompatible, solanaceous plants, such asNicotiana alata, and are thought to be involved in selfpollen rejection by acting selectively as toxins to selfpollen. In this study, the toxicity of RNases to other plant cells was tested by culturing cells ofN. alata andN. plumbaginifolia in the presence ofS-RNases fromN. alata. The growth of cultured cells ofN. plumbaginifolia was inhibited by theS-RNases, but viability was not affected. Growth of cultured cells of oneN. alata selfincompatibility genotype was inhibited by twoS-RNases, indicating that inhibition was not allele specific. Comparisons with the effects of inactivated RNase and other proteins, suggest that the inhibition of growth byS 2-RNase was partly, but not wholly, due to RNase activity. Heat-denaturedS 2-RNase was a very effective inhibitor of cell growth, but this inhibitory activity may be a cell surface phenomenon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344356
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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