Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray crystallographic analysis of S-allelic glycoprotein SF11-RNase from Nicotiana alata (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 143-144 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Nicotiana alata SF11-RNase is an S-glycoprotein associated with gametophytic self-incompatibility. Crystals of SF11-RNase have been grown at room temperature using polyethylene glycol as a precipitant. A crystal diffracted to better than 1.4 Å resolution at 100 K at the SPring-8 synchrotron-radiation source, indicating that it is very suitable for high-resolution structure analysis. The crystal belongs to the space group P21, with unit-cell parameters a = 65.86 (11), b = 44.73 (5), c = 64.36 (7) Å, β = 90.27 (9)°. The asymmetric unit contains two monomers, giving a crystal volume per protein mass (VM) of 2.05 Å3 Da−1 and a solvent content of 39.6% by volume. A full set of X-ray diffraction data was collected to 1.55 Å resolution with a completeness of 97.4%. A heavy-atom derivative has been successfully prepared with ethylmercury thiosalicylate (EMTS) and structure analysis is in progress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444900014050
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Ca2+ -dependent protein phosphorylation in germinated pollen of Nicotiana alata, an ornamental tobacco (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 67 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The 40 000 g supernatant and 40 000 g pellet from extracts of germinated pollen of Nicotiana alata Link et Otto contain protein kinase activity which catalyzes the phosphorylation of histones, casein and a range of endogenous polypeptides. Phosphorylation of certain low-molecular-weight, casein-derived polypeptides is activated at low (12–37 μM) and partially inhibited at higher (540 μM) concentrations of free Ca2+. Histone phosphorylation is largely Ca2+-dependent and is activated by 540 μM free Ca2+. No activation of protein phosphorylation by micromolar concentrations of calmodulin is found, but phenothiazine-derived calmodulin antagonists markedly stimulate protein phosphorylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb02437.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Growth inhibition of suspension cultured plant cells by ribonucleases (1995)
    Springer
    Journal of plant research 108 (1995), S. 305-312 
    Details
    ISSN: 1618-0860
    Keywords: Nicotiana alata ; Nicotiana plumbaginifolia ; Pollen ; Ribonuclease ; Self-incompatibility ; Toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracellular, stylar RNases (S-RNases) are produced by self-incompatible, solanaceous plants, such asNicotiana alata, and are thought to be involved in selfpollen rejection by acting selectively as toxins to selfpollen. In this study, the toxicity of RNases to other plant cells was tested by culturing cells ofN. alata andN. plumbaginifolia in the presence ofS-RNases fromN. alata. The growth of cultured cells ofN. plumbaginifolia was inhibited by theS-RNases, but viability was not affected. Growth of cultured cells of oneN. alata selfincompatibility genotype was inhibited by twoS-RNases, indicating that inhibition was not allele specific. Comparisons with the effects of inactivated RNase and other proteins, suggest that the inhibition of growth byS 2-RNase was partly, but not wholly, due to RNase activity. Heat-denaturedS 2-RNase was a very effective inhibitor of cell growth, but this inhibitory activity may be a cell surface phenomenon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344356
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Self-incompatibility in Nicotiana alata involves degradation of pollen rRNA (1990)
    [s.l.] : Nature Publishing Group
    Nature 347 (1990), S. 757-760 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The finding that style S-glycoproteins from N. alata are RNases11 leads to the question of whether this enzymatic function has a role in expression of self-incompatibility (SI). To approach this question we obtained labelled pollen by growing plants in the presence of 32P and followed the fate of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/347757a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Style self-incompatibility gene products of Nicotlana alata are ribonucleases (1989)
    [s.l.] : Nature Publishing Group
    Nature 342 (1989), S. 955-957 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cloned complementary DNAs corresponding to the 52-, S3~ and S6-allelic glycoproteins6 have recently been isolated2'3. The primary amino-acid sequences of three S-glycoproteins show some regions that are perfectly conserved and other regions that are less homologous3. Sequence analysis reveals ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/342955a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Proline- and hydroxyproline-rich gene products in the sexual tissues of flowers (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 253-260 
    Details
    ISSN: 1432-2145
    Keywords: Key words Hydroxyproline-rich glycoproteins ; Proline/hydroxyproline-rich glycoproteins ; Extensin ; Arabinogalactan-proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This review summarises information on hydroxyproline-rich glycoproteins (HRGPs) and clones encoding these molecules isolated from male and female tissues of flowering plants. HRGPs are abundant in sexual tissues and a number of different HRGPs have been isolated. The protein and carbohydrate components of these HRGPs have been characterised and cDNAs encoding the protein backbones of several have been isolated and sequenced. Further work is directed at detailed structural analysis of the carbohydrate side chains of these molecules and their points of linkage to the protein backbones. The biological functions of most of these molecules have not yet been established unequivocally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050095
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Localisation and expression of arabinogalactan-proteins in the ovaries of Nicotiana alata Link and Otto (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 278-282 
    Details
    ISSN: 1432-2145
    Keywords: Arabinogalactan-proteins ; Ovary ; Nicotiana ; Pollination ; Flower development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The transmitting-tissue cells of the style of flowering plants secrete a complex extracellular matrix through which pollen tubes grow to the ovary to effect fertilisation. This matrix is particularly rich in a class of proteoglycans, the arabinogalactan-proteins (AGPs). AGPs from the ovary of Nicotiana alata were found to be developmentally regulated, as the different charge classes of AGPs altered during floral development. The AGPs from the mature ovary had charge characteristics that were distinct from those previously reported for the stigma and style. However, the concentration of AGP (0.6 μg/ml fresh weight) in the ovary did not change during development, or in response to either compatible or incompatible pollination. The AGPs of the ovary are mainly associated with the epidermis of the placenta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229384
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    An in vitro assay for assessing the effects of growth factors on Nicotiana alata pollen tubes (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 351-357 
    Details
    ISSN: 1432-2145
    Keywords: Key words Bioassay ; Extracellular matrix ; Nicotiana alata ; Pollen density ; Polyethylene glycol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A new method for assessing the effects of test compounds on Nicotiana alata pollen tubes in culture is described. Pollen tubes grow from a cluster of grains placed beneath a thin layer of gelled medium in which test substances are incorporated and from which evaporation is prevented by a covering layer of oil. Pollen tubes can grow to 8 mm in length in 24 h, which corresponds to about 25% of the maximum growth rate in styles. Growth is non-destructively measured. The developmental stages reached by cultured tubes are similar to those of tubes growing in styles; growth changes from being reserve-dependent to reserve-independent, callose plugs form, and the nucleus of the generative cell divides. Because culture volumes are small (10–20 μl per replicate), the effects of known concentrations of microgram quantities of compounds on the growth of pollen tubes can be tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050109
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    A style-specific 120-kDa glycoprotein enters pollen tubes ofNicotiana alata in vivo (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 75-86 
    Details
    ISSN: 1432-2145
    Keywords: Hydroxyproline-rich glycoprotein ; Pistil ; Pollen tubes ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pistils ofNicotiana alata (Link et Otto) contain an abundant, style-specific glycoprotein (120 kDa) that is rich in hydroxyproline and has both extensin-like and arabinogalactan-protein-like carbohydrate substituents. An antibody specific for the protein backbone of the glycoprotein was used to localise the glycoprotein in both unpollinated and pollinated pistils. The glycoprotein is evenly distributed in the extracellular matrix of the style transmitting tract of unpollinated pistils and, despite the presence of extensin-like carbohydrate substituents, is not associated with the walls of the transmitting tract cells. In pollinated pistils the 120-kDa glycoprotein is concentrated in the extracellular matrix adjacent to pollen tubes, and is also present in the cytoplasm and the cell walls of pollen tubes. Pollen tubes grown in vitro do not contain the 120-kDa glycoprotein unless it is added to the growth medium, suggesting that the 120kDa glycoprotein located in pistil-grown pollen tubes is derived from the extracellular matrix of the transmitting tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02153054
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Observations of pollen tube growth in Nicotiana alata and their implications for the mechanism of self-incompatibility (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 27-35 
    Details
    ISSN: 1432-2145
    Keywords: Key words Nicotiana alata ; Pollen tube growth ; Stylar graft ; Self-incompatibility ; Cytotoxic model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Style squashes and stylar grafts were used to examine the growth of Nicotiana alata pollen tubes in self-compatible and self-incompatible styles. Compatible tubes typically showed a uniform layer of callose deposition in the walls and in small plugs spaced at regular intervals within the tube. Incompatible tubes were characterised by the variability of callose deposition in the walls and by larger, closer and more irregularly spaced plugs. There was no difference in the growth rate of compatible and incompatible tubes during growth through the stigma, but within the style most compatible tubes grew 20–25 mm day-1 (maximum 30 mm day–1), whereas incompatible tubes grew 1.0–1.5 mm day-1 (maximum 5 mm day–1). Many incompatible tubes continued to grow until flowers senesced, and only a small proportion died as a consequence of tip bursting. Grafting compatibly pollinated styles onto incompatible styles showed that the incompatible reaction could occur in pollen tubes between 2 and 50 mm long, and that inhibition of pollen tube growth occurred in both the upper and lower parts of the transmitting tract. Grafting incompatibly pollinated styles onto compatible styles showed that the incompatible reaction was fully reversible in at least a proportion of the pollen tubes. The findings are not consistent with the cytotoxic model of inhibition of self-pollen tubes in solanaceous plants, which assumes that the incompatible response results from the degradation of a finite amount of rRNA present in the pollen tube. However, if pollen tubes do in fact synthesise rRNA, the findings become consistent with this model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050064
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...