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  • 1
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray crystallographic analysis of S-allelic glycoprotein SF11-RNase from Nicotiana alata (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 143-144 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Nicotiana alata SF11-RNase is an S-glycoprotein associated with gametophytic self-incompatibility. Crystals of SF11-RNase have been grown at room temperature using polyethylene glycol as a precipitant. A crystal diffracted to better than 1.4 Å resolution at 100 K at the SPring-8 synchrotron-radiation source, indicating that it is very suitable for high-resolution structure analysis. The crystal belongs to the space group P21, with unit-cell parameters a = 65.86 (11), b = 44.73 (5), c = 64.36 (7) Å, β = 90.27 (9)°. The asymmetric unit contains two monomers, giving a crystal volume per protein mass (VM) of 2.05 Å3 Da−1 and a solvent content of 39.6% by volume. A full set of X-ray diffraction data was collected to 1.55 Å resolution with a completeness of 97.4%. A heavy-atom derivative has been successfully prepared with ethylmercury thiosalicylate (EMTS) and structure analysis is in progress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444900014050
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  • 2
    Electronic Resource
    Electronic Resource
    Plant reproduction Sex and self-denial (2003)
    [s.l.] : Nature Publishing Group
    Nature 423 (2003), S. 229-230 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Ubiquitin is certainly living up to its name. This small protein, first named because of its seemingly ubiquitous presence in animal and plant cells, now seems to be an almost universal regulator molecule. Ubiquitin attaches to a multitude of other proteins, and its well-known role is as a tag that ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/423229a
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  • 3
    Electronic Resource
    Electronic Resource
    The evolution of self-incompatibility: a molecular voyeur's perspective (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 357-361 
    Details
    ISSN: 1432-2145
    Keywords: Self-incompatibility ; Evolution ; S-RNases ; Solanaceae ; Rosaceae ; Scrophulariaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This review summarises current understanding of the evolution of self-incompatibility inferred from DNA sequence analysis. Self-incompatibility in many plant families is controlled by a single, highly polymorphicS-locus which, in the Solanaceae, encodes an allelic series of stylar ribonucleases known as the S-RNases. PCR approaches are a convenient way to examine the diversity of S-RNase sequences within and between wild populations of a self-incompatible species and provide a unique view into the species' current and historic population structure. Similar molecular appoaches have also been used to show that S-RNases are involved in self-incompatibility in families other than the Solanaceae. A model for the evolution of ribonuclease-based self-incompatibility systems is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441956
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  • 4
    Electronic Resource
    Electronic Resource
    The S-locus of Nicotiana alata: genomic organization and sequence analysis of two S-RNase alleles (1995)
    Springer
    Plant molecular biology 28 (1995), S. 847-858 
    Details
    ISSN: 1573-5028
    Keywords: Nicotiana alata ; repetitive DNA ; self-incompatibility ; S-RNase gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic clones encoding the S 2- and S 6-RNases of Nicotiana alata Link and Otto, which are the allelic stylar products of the self-incompatibility (S) locus, were isolated and sequenced. Analysis of genomic DNA by pulsed-field gel electrophoresis and Southern blotting indicates the presence of only a single S-RNase gene in the N. alata genome. The sequences of the open-reading frames in the genomic and corresponding cDNA clones were identical. The organization of the genes was similar to that of other S-RNase genes from solanaceous plants. No sequence similarity was found between the DNA flanking the S 2- and S 6-RNase genes, despite extensive similarities between the coding regions. The DNA flanking the S 6-RNase gene contained sequences that were moderately abundant in the genome. These repeat sequences are also present in other members of the Nicotianae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042070
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular characterisation of an S-like RNase of Nicotiana alata that is induced by phosphate starvation (1996)
    Springer
    Plant molecular biology 31 (1996), S. 227-238 
    Details
    ISSN: 1573-5028
    Keywords: Nicotiana alata ; phosphate starvation ; RNase ; self-incompatibility ; SINE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We characterised a cDNA encoding an S-like RNase (RNase NE) from the styles of the self-incompatible plant, Nicotiana alata. RNase NE is 86% identical to an extracellular RNase from tomato cell cultures, RNase LE. DNA hybridisation experiments indicate that there are ca. 5–6 sequences related to RNase NE in the N. alata genome and that RNase NE is not linked to the self-incompatibility (S) locus. RNase NE is expressed in the styles, petals and immature anthers but not in the vegetative tissues of N. alata plants under normal growth conditions. Under phosphate-limited conditions, RNase NE expression is induced in roots but not leaves of N. alata. A transcript hybridising to RNase NE is also induced in N. plumbaginifolia cell cultures in response to phosphate starvation. RNase NE is likely to play a role in the response of N. alata to phosphate limitation, possibly by scavenging phosphate from sources of RNA in the root environment. We also discuss the evolutionary relationships between the S- and S-like RNase genes in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021786
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  • 6
    Electronic Resource
    Electronic Resource
    A retrotransposon-like sequence linked to the S-locus ofNicotiana alata is expressed in styles in response to touch (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 180-188 
    Details
    ISSN: 1617-4623
    Keywords: gypsy-like retrotransposon ; Nicotiana alata ; S-locus ; Touch-inducible ; Pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified a family of repetitive sequences in the genome ofNicotiana alata namedTnal (Transposon ofN. alata). The first element we characterised was on a genomic clone for theN. alata S6-ribonuclease (S6-RNase), a gene required for self-incompatibility in this species. The DNA sequence of this element resembles the integrase domain of retro-transposons of thegypsy class and is most similar to a retrotransposon fromLilium henryi. A transcript present inN. alata styles (self-incompatibility genotype S6S6) hybridized toTnal and accumulated in the style following either pollination or touching. This transcript was cloned from a cDNA library and was encoded by a second, partialTnal element. Neither the transcribed sequence nor the originalTnal element contain an open reading frame or is likely to be able to transpose. The second element was mapped using a population ofN. alata plants segregating for alleles of the self-incompatibility locus and is closely linked to the S6-allele. TheTnal element is present in a number ofNicotiana species and appears to have been active at least twice during the evolution of this genus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174177
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  • 7
    Electronic Resource
    Electronic Resource
    Transgenic tobacco plants which express thechiA gene fromSerratia marcescens have enhanced tolerance toRhizoctonia solani (1994)
    Springer
    Transgenic research 3 (1994), S. 90-98 
    Details
    ISSN: 1573-9368
    Keywords: Bacterial chitinase ; chiA ; transgenic tobacco ; Rhizoctonia solani
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have prepared independent lines of transgenic tobacco plants which express high levels of theSerratia marcescens ChiA protein intracellulary or extracellularly (in glycosylated or unglycosylated forms). We have measured the susceptibility, of these plants toRhizoctonia solani infection in greenhouse trials and in the field. Transgenic tobacco plants which constitutively express theS. marcescens ChiA protein exhibit tolerance to the fungal pathogenR. solani. Disease tolerance is observed in transgenic tobacco plants which express the bacterial chitinase intra-or extracellulary. This is the first report to document disease reduction in the field in transgenic plants engineered for fungal disease tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01974086
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