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  • Key words Root architecture  (1)
  • Melampsora larici-populina  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Lettuce, a shallow-rooted crop, and Lactuca serriola, its wild progenitor, differ at QTL determining root architecture and deep soil water exploitation (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1066-1073 
    Details
    ISSN: 1432-2242
    Keywords: Key words Root architecture ; Soil water ; Quantitative trait loci ; Crop domestication ; AFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Wild plant species are often adapted to more stressful environments than their cultivated relatives. Roots are critical in exploiting soil resources that enable plants to withstand environmental stresses, but they are difficult to study. Cultivated lettuce (Lactuca sativa L.) and wild L. serriola L. differ greatly in both shoot and root characteristics. Approximately 100 F2:3 families derived from an interspecific cross were evaluated in greenhouse and field experiments. In the greenhouse, root traits (taproot length, number of laterals emerging from the taproot, and biomass) and shoot biomass were measured 4 weeks after planting. In the field, plants were grown for 9 weeks (close to harvest maturity of the cultivated parent); mild drought stress was induced by withholding water for 1 week, and gravimetric moisture of soil was then determined for five depth increments between 0–100 cm. The families were genotyped using codominantly scored AFLP markers distributed throughout the genome. Composite interval mapping was used to analyze marker-trait associations. Quantitative trait loci were identified for differences between wild and cultivated lettuce for root architectural traits and water acquisition. Thirteen QTL were detected that each accounted for 28–83% of the phenotypic variation. The loci for taproot length (i.e., cm taproot length g–1 plant biomass) and the ability to extract water from deep in the soil profile co-localized in the genome. These coincident loci were identified in separate experiments. The wild L. serriola is therefore a potential source of agriculturally important alleles to optimize resource acquisition by cultivated lettuce, thereby minimizing water and fertilizer inputs and ultimately enhancing water quality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051581
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of AFLP molecular markers for resistance against Melampsora larici-populina in Populus (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 733-737 
    Details
    ISSN: 1432-2242
    Keywords: Mer ; AFLP™ markers ; Bulked segregant analysis ; Melampsora larici-populina ; Populus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified AFLP markers tightly linked to the locus conferring resistance to the leaf rust Melampsora larici-populina in Populus. The study was carried out using a hybrid progeny derived from an inter-specific, controlled cross between a resistant Populus deltoides female and a susceptible P. nigra male. The segregation ratio of resistant to susceptible plants suggested that a single, dominant locus defined this resistance. This locus, which we have designated Melampsora resistance (Mer), confers resistance against E1, E2, and E3, three different races of Melampsora larici-populina. In order to identify molecular markers linked to the Mer locus we decided to combine two different techniques: (1) the high-density marker technology, AFLP, which allows the analysis of thousands of markers in a relatively short time, and (2) the Bulked Segregant Analysis (BSA), a method which facilitates the identification of markers that are tightly linked to the locus of interest. We analyzed approximately 11,500 selectively amplified DNA fragments using 144 primer combinations and identified three markers tightly linked to the Mer locus. The markers can be useful in current breeding programs and are the basis for future cloning of the resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224069
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    Paper (German National Licenses)
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