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  • 1
    Electronic Resource
    Electronic Resource
    A genetic map of Maritime pine based on AFLP, RAPD and protein markers (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 39-48 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pinus pinaster ; AFLP ; RAPD ; Protein ; Linkage map ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  TheAFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species characterized by a large genome size (24 pg/C). A genetic linkage map was constructed for one F1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphic DNA) markers. Markers were scored on megagametophytes (1n) from 200 germinated F2 seedlings. Polymorphism rate, labour time and cost of both AFLP and RAPD techniques were compared. The AFLP technique was found to be twice as fast and three-times less costly per marker than the RAPD technique. Thirteen linkage groups were identified with a LOD score ≥6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins were extracted from needles (2n) of the F2 progeny and revealed by 2-DE (two-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection strategy based on the quantification of protein accumulation. Two framework maps of the same F1 individual are now available. The first map (Plomion et al. 1996) uses RAPD markers and the second map, presented in this study, uses mostly AFLP markers. Although the total genetic length of both maps was almost identical, differences among homologous groups were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050006
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of AFLP molecular markers for resistance against Melampsora larici-populina in Populus (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 733-737 
    Details
    ISSN: 1432-2242
    Keywords: Mer ; AFLP™ markers ; Bulked segregant analysis ; Melampsora larici-populina ; Populus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified AFLP markers tightly linked to the locus conferring resistance to the leaf rust Melampsora larici-populina in Populus. The study was carried out using a hybrid progeny derived from an inter-specific, controlled cross between a resistant Populus deltoides female and a susceptible P. nigra male. The segregation ratio of resistant to susceptible plants suggested that a single, dominant locus defined this resistance. This locus, which we have designated Melampsora resistance (Mer), confers resistance against E1, E2, and E3, three different races of Melampsora larici-populina. In order to identify molecular markers linked to the Mer locus we decided to combine two different techniques: (1) the high-density marker technology, AFLP, which allows the analysis of thousands of markers in a relatively short time, and (2) the Bulked Segregant Analysis (BSA), a method which facilitates the identification of markers that are tightly linked to the locus of interest. We analyzed approximately 11,500 selectively amplified DNA fragments using 144 primer combinations and identified three markers tightly linked to the Mer locus. The markers can be useful in current breeding programs and are the basis for future cloning of the resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224069
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Application of AFLPs to the characterization of grapevine Vitis vinifera L. genetic resources. A case study with accessions from Rioja (Spain) (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 51-59 
    Details
    ISSN: 1432-2242
    Keywords: Key words Vitis vinifera ; AFLPs ; Cultivar identification ; Clone identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  AFLPs were used to characterize 67 different grapevine accessions from a collection of D.O.Ca. Rioja in Spain. A correct selection of primers and selective nucleotides allowed us to maximize the number of amplified fragments analyzed per reaction yielding an average of 100 per reaction, 49% of which were polymorphic. Based on the presence or absence of amplified fragments for each genotype resulting from a reaction with two primer combinations, we have established the genetic similarity between the different accessions in the collection. These results allowed us to resolve different genotypes maintained under the same name (homonyms) and to identify the same genotype under different names (synonyms) thus permitting the elimination of redundant germplasm. Furthermore, by providing information on more than 50 polymorphic loci per reaction, a few reactions were sufficient to identify distinct AFLP patterns characteristic of specific clones, with different agronomic and organoleptic features, belonging to the same cultivar. The possibility for clonal identification, shown here for grapevines, can have important implications in the protection and management of clonal selections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050866
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    Paper (German National Licenses)
    Fulltext
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