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Development of a rapid assay for screening point mutations associated with quinolone resistance in the Pseudomonas aeruginosa parC gene (2000)

Deguchi, Takashi ; Yamaha, Masayoshi ; Nakano, Masahiro ; [et al.]

Springer

Journal of infection and chemotherapy 6 (2000), S. 26-29

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ISSN: 1437-7780

Keywords: Key wordsPseudomonas aeruginosa ; parC mutations ; Fluoroquinolone resistance

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract To detect quinolone resistance-associated mutations within the Ser-80 and Glu-84 codons of the Pseudomonas aeruginosa parC gene, we developed a rapid and simple assay based on polymerase chain reaction (PCR) amplification of the region of the parC gene containing the mutation sites and digestion of the PCR products with a restriction enzyme. The mutations generating alterations at Ser-80 and Glu-84 were detected as restriction fragment length polymorphisms of the PCR products digested with HinfI. Among 22 clinical isolates tested by this assay, mutations at the Ser-80 and Glu-84 codons were detected in all 10 isolates in which the presence of the mutations had been confirmed previously by DNA sequencing. This rapid and simple assay could be a useful screening device for genetic alterations associated with resistance to quinolones in the P. aeruginosa parC gene.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s101560050045

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Release characteristics of dibucaine dispersed in konjac gels (1979)

Nakano, Masahiro ; Takikawa, Kaori ; Arita, Takaichi

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 13 (1979), S. 811-819

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ISSN: 0021-9304

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: A possible use of konjac gel for sustained release of drugs was examined in a monolithic system containing dibucaine. Dibucaine was dispersed in the gel which was prepared by gelation of the konjac flour in a borax solution at 60°. The cumulative amount of the drug released plotted against the square root of time was linear in the monolithic system. This relationship was in agreement with that expected from the theoretical equation for planar configuration. The mechanism of the release of the drug from the gel may be considered to be a leaching of the drug by the permeating fluid. The release profile from dried konjac gel was similar to that from undried gel, but that from unwarmed gel showed a deviation from linearity although sustained release was similarly obtained.

Additional Material: 5 Ill.