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  • Polymer and Materials Science  (3)
  • Physical mapping  (1)
  • Plasma  (1)
  • 1
    ISSN: 1432-2242
    Keywords: Genomic probing ; In situ hybridization ; Interphase cytogenetics ; Physical mapping ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genomic in situ hybridization was used to identify alien chromatin in chromosome spreads of wheat, Triticum aestivum L., lines incorporating chromosomes from Leymus multicaulis (Kar. and Kir.) Tzvelev and Thinopyrum bessarabicum (Savul. and Rayss) Löve, and chromosome arms from Hordeum chilense Roem. and Schult, H. vulgare L. and Secale cereale L. Total genomic DNA from the introgressed alien species was used as a probe, together with excess amounts of unlabelled blocking DNA from wheat, for DNA:DNA in-situ hybridization. The method labelled the alien chromatin yellow-green, while the wheat chromosomes showed only the orange-red fluorescence of the DNA counterstain. Nuclei were screened from seedling root-tips (including those from half-grains) and anther wall tissue. The genomic probing method identified alien chromosomes and chromosome arms and allowed counting in nuclei at all stages of the cell cycle, so complete metaphases were not needed. At prophase or interphase, two labelled domains were visible in most nuclei from disomic lines, while only one labelled domain was visible in monosomic lines. At metaphase, direct visualization of the morphology of the alien chromosome or chromosome segment was possible and allowed identification of the relationship of the alien chromatin to the wheat chromosomes. The genomic in-situ hybridization method is fast, sensitive, accurate and informative. Hence it is likely to be of great value for both cytogenetic analysis and in plant breeding programmes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: To gain a better understanding of the evolution of polymer surfaces under cold plasmas, model polymer surfaces were studied. The degradation products and the gas phase were investigated by mass and optical emission spectrometry. Their evolution versus time and power enable us to propose a mechanism that involves atomic oxygen, OH* and H* radicals. © 1992 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 46 (1992), S. 2063-2074 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: This work deals with the influence of pressure, gas flow rate, and gas mixture on the etching rates of polymer model molecules such as hexatriacontane (C36H74) and octadecyl octadecanoate (OOD) treated in an oxygen or argon plasma. Mass spectrometry and optical emission spectroscopy have been used to monitor the formation and evolution of the fragments coming from chain breaking in terms of their concentration and emission intensity, respectively. It was demonstrated that pressure and gas flow rate are two important parameters in the interaction of cold plasmas and of polymer surfaces. The results obtained can be explained by combined effects such as electron energy and its density, residence time, “quenching effect,” as well as chemical reactions. © 1992 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plasmas and polymers 4 (1999), S. 247-258 
    ISSN: 1572-8978
    Keywords: Plasma ; polymer etching ; in situ detection ; PET and acrylic coating
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Residual gas analysis (RGA) and optical emission spectroscopy have been evaluated as potential in situ techniques for the detection of plasma-induced polymer surface etching. The detection is based on the measurement of CO and CO2 species formed in the gas phase following oxidation of the etching fragments released from the polymer surface. Experiments were performed on poly(ethylene terephthalate) and UV-cured acrylic (tripropylene glycol diacrylate) films exposed to O2 RF (13.56 MHz) plasmas. A linear correlation is obtained between the formation of CO and the polymer etching rate over the entire experimental range, but discrepancies appear for the formation of CO2 at high treatment powers (etching rate 〉 1.0 μg/min.cm2). This behavior is attributed to a deficit of oxidizing agents relative to the generation of etching fragments. The results suggest that both RGA and optical emission spectroscopy can be used to monitor in situ and in real-time the etching of polymer surfaces during plasma treatment.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 23 (1995), S. 99-104 
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Teflon PFA (polytetrafluoroethylene-co-perfluoroalkoxy vinyl ether) was treated by a low-pressure microwave (2.45 GHz) plasma in O2, N2, H2, He and their mixtures. X-ray photoelectron spectroscopy measurements showed ablation of fluorine and incorporation of new O- and N-containing groups into the surface. Among the different gases, H2 is the most efficient for defluorination, N2 + H2 for surface functionalization, while O2 showed the least performance for both effects. Plasma treatments using N2 + H2 and O2 + H2 resulted in intermediate defluorination, i.e. between those in the pure gases, and sequential treatment by He/(N2 + H2) showed an additive effect of He and N2 + H2 alone. Angle-resolved XPS measurements revealed a pronounced gradient in the near-surface composition following H2, N2 + H2 and He/(N2 + H2) plasma treatments, in contrast to a more uniform depth distribution upon O2, N2 and He exposures. The surface modification of Teflon PFA leads to an enhanced adhesion of metals such as Cu, Ag and Au.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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