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  • Plant tissue culture  (1)
  • soft coral  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Structures and possible functions of epoxypukalide and pukalide (1989)
    Springer
    Journal of chemical ecology 15 (1989), S. 1177-1191 
    Details
    ISSN: 1573-1561
    Keywords: Sinularia spp. ; spawning ; soft coral ; pukalide ; epoxypukalide ; structure determination ; diterpene ; octocorals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chemical analysis of the eggs spawned by fiveSinularia soft corals (Octocorallia, Alcyoniidae) collected in the central region of the Great Barrier Reef, Australia, revealed the presence of the known cembranoid diterpenes epoxypukalide and/or pukalide in all cases. Examination of the colonies from which the eggs were released one month before spawning and also some time after spawning failed to detect either diterpene in the coral tissue. The three dimensional structures of pukalide and epoxypukalide, as determined by the single-crystal X-ray technique, are reported for the first time. The wide distribution of pukalide and epoxypukalide and of related compounds within the octocorallia is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01014821
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Position of water in vitrified plants visualised by NMR imaging (1998)
    Springer
    Protoplasma 201 (1998), S. 110-114 
    Details
    ISSN: 1615-6102
    Keywords: Nuclear magnetic resonance imaging ; Plant tissue culture ; Vitrification ; Gypsophila paniculata ; Intercellular air spaces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitrification of plants in vitro is a physiological abnormality of tissue-cultured plants which causes significant losses in the micropropagation industry. Vitrified plants are waterlogged but the position of water within plants has not been identified. Nuclear magnetic resonance (NMR) imaging of normal tissue-cultured, vitrified tissue-cultured, and glasshouse-grown leaves ofGypsophila paniculata showed the distribution of water within the leaves. Normal tissue-cultured and glasshouse-grown leaves had a high concentration of water within leaf vascular bundles and lower concentrations elsewhere. In contrast, vitrified leaves had a relatively even distribution of high water concentration throughout the leaves. When imaging parameters were changed, so that only water associated with cell membranes was shown, the images of normal tissue-cultured and glasshouse-grown leaves did not change. However, the image of the vitrified leaves showed a general lowering of intensity across the whole of the leaf. The appearance of the NMR images, together with those obtained by light microscopy, suggest that the excess water associated with vitrified plants is located in the intercellular air spaces. The blockage of these spaces may lead to a cycle of perturbations in the plant's physiology culminating in the development of vitrification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280717
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    Paper (German National Licenses)
    Fulltext
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