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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 30 (1991), S. 123-130 
    ISSN: 1573-5079
    Keywords: oxygen evolution ; calcium ; chloride ; electron transport ; photosynthetic inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inhibitory effect of Zn2+ on photosynthetic electron transport was investigated in native and CaCl2-treated (depleted in extrinsic polypeptides) Photosystem II (PS II) submembrane preparations. Inhibition of 2,6-dichlorophenolindophenol photoreduction by Zn2+ was much stronger in protein-depleted preparations in comparison to the native form. It was found that Ca2+ significantly reduced the inhibition in the native PS II preparations, as did Mn2+ in a combination with H2O2 in the protein-depleted counterparts. No other tested monovalent or divalent cations could replace Ca2+ or Mn2+ in the respective experiments. Diphenylcarbazide could partially relieve (40–45%) the inhibition in both types of preparations. The above indicates the presence of an active Zn2+ inhibitory site on the donor side of PS II. However, neither Ca2+ nor Mn2+ could completely prevent inhibition by high concentrations of Zn2+ (〉1 mM). We propose that elevated levels of Zn2+ strongly perturb the conformation of the PS II core complex and might also affect the acceptor side of the photosystem.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5079
    Keywords: atrazine ; calcium ; chloride ; depleted-photosystem II ; DCIP-photoreduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The oxygen evolving complex of photosystem II (PS II) contains three extrinsic polypeptides of approximate molecular weights 16, 23 and 33 kDa. These polypeptides are associated with the roles of Cl-, Ca2+ and Mn2+ in oxygen evolution. We have shown that selective removal of 16 and 23 kDa polypeptides from the above complex by NaCl washing of PS II enriched membrane fragments renders the PS II core complex more susceptible to the herbicide atrazine. On the other hand, when both native and depleted preparations were resupplied with exogenous Ca2+ and Cl-, we obtained a reduction of atrazine inhibition which was much stronger in the depleted preparations than in the native ones. It is concluded that removal of 16 and 23 kDa polypeptides in general, and disorganization of associated Ca2+ and Cl- in particular, enhances atrazine penetration to its sites of action in the vicinity of the PS II complex. The above could be interpreted if we assume a reduced plastoquinone affinity at the QB (secondary plastoquinone electron acceptor) pocket of D1 polypeptide following transmembranous modifications caused by the depletion of these polypeptides.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 40 (1994), S. 263-268 
    ISSN: 1573-5079
    Keywords: photoacoustie spectroscopy ; Photosystem I ; electron transport ; thermal dissipation ; plastoquinone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photoacoustic spectroscopy was used to study the thermal deactivation processes in a Photosystem I submembrane fraction isolated from spinach. A large part of the thermal dissipation was variable. The yield of this variable thermal emission depended on the redox state of the Photosystem. It increased with the measuring modulated light intensity coinciding with the gradual closure of the reaction centers. Thermal deactivation was maximal when the reaction centers were closed by a saturating illumination. Extrapolation of the data at zero light intensity indicated that the yield of non-variable thermal emission represented about 37% of the maximal emission. The presence of methylviologen as artificial electron acceptor decreased the yield of variable thermal emission whereas inhibition following heat stress treatments increased it. The significance of the variable and non-variable components of thermal dissipation is discussed and the measured energy storage is suggested to originate from the reduction of the plastoquinone pool during cyclic electron transport around Photosystem I.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5079
    Keywords: cyclic electron transport ; fluorescence ; metal ions ; Photosystem II ; thermal dissipation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simultaneous fluorescence and photoacoustic measurements have been used to study the effects of metal ions (copper, lead, and mercury) during dark incubation of thylakoid membranes. The values of the chlorophyll fluorescence parameters Fo (initial fluorescence yield with the reaction centers in the open state), Fm (maximal fluorescence yield), Ft (steady state fluorescence yield) and the calculated parameters, Φo (maximal quantum yield of Photosystem II photochemistry) and Φt (actual quantum yield of Photosystem II photochemistry), strongly decreased in the presence of the metal ions coinciding with an increase in the non-photochemical deexcitation rate constant k(N). It was observed that photosynthetic energy storage measured by photoacoustic spectroscopy also decreased but a large portion of energy storage remained unaffected even at the highest metal ion concentrations used. A maximal inhibition of photosyntheti c energy storage of 80% and 50% was obtained with Hg2+ and Cu2+-treated thylakoids, respectively, while energy storage was insensitive to Pb2+. The results are consistent with the known predominant inhibition of the donor side of Photosystem II by the metal ions. The insensitive portion of energy storage is attributed to the possible recurrence of cyclic electron transport around Photosystem II that would depend on the extent of inhibition produced on the acceptor side by the metal ion used.
    Type of Medium: Electronic Resource
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