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  • 1
    Electronic Resource
    Electronic Resource
    Cross-sectional transmission electron microscopy study of isolated diamond particles grown on a mirror-polished Si substrate (1995)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 67 (1995), S. 632-634 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Isolated diamond particles grown by chemical vapor deposition on a mirror-polished Si substrate have been studied by cross-sectional transmission electron microscopy. Focused ion beam micromachining enabled the cross-sectional specimen to be carved out precisely at the center of the particle. Atomic scale observation of the diamond/Si interface revealed the presence of an ∼3 nm thick amorphous intermediate layer including a few pits around the nucleation site of the particle. Growth mechanism and relationship between growth orientation and internal defect structure of the diamond particle are discussed. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.115185
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  • 2
    Electronic Resource
    Electronic Resource
    Transmission electron microscopy study of interface and internal defect structures of homoepitaxial diamond (1996)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 68 (1996), S. 2070-2072 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Defect structures in a homoepitaxial diamond film grown by chemical vapor deposition have been studied by cross-sectional transmission electron microscopy. Many interstitial dislocation loops are discerned in the (001) interface. The internal region grown on the (11¯1) facet comprises stacking faults and twins, while that on the (001) face contains mainly interstitial dislocation loops aligned in rows along ∼〈112〉 directions. Fe and Si impurities were detected only at the interface by analytical electron microscopy. The origin of the defects is briefly discussed. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.116306
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  • 3
    Electronic Resource
    Electronic Resource
    Surface morphology and growth mechanism of YBa2Cu3O7−y films by metalorganic chemical vapor deposition using liquid sources (1996)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 69 (1996), S. 845-847 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have investigated surface morphology of YBa2Cu3O7−y thin films prepared by chemical vapor deposition (CVD) using liquid metalorganic (MO) sources on MgO(100) single crystalline substrates by atomic force microscopy (AFM). An abrupt change in the terrace width was observed at the deposition temperature of around 750 °C. An anomalous decrease in the efficiency of incorporation of the yttrium component into the film was also found above the same temperature. It suggests that the appearance of liquid phase on the growing surface and the growth mode change from the conventional vapor growth to the VLS (vapor–liquid–solid) growth mode at this temperature. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.117911
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  • 4
    Electronic Resource
    Electronic Resource
    Inhaled corticosteroids decrease vascularity of the bronchial mucosa in patients with asthma (2001)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 31 (2001), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Increased vascularity in airway mucosa is a distinctive feature of airway remodelling in asthma. While corticosteroids have proved most effective in modifying airway inflammation, the effect of inhaled corticosteroids on increased airway mucosal vascularity in asthmatics has been little studied.Objective We examined the effect of inhaled corticosteroid on airway vascularity in bronchial biopsy specimens taken from asthmatic patients.Subjects and methods We studied bronchial biopsies from 28 asthmatic patients before and after treatment with inhaled beclomethasone dipropionate (BDP) 800 µg/daily, or placebo, for 6 months in a double-blind manner. Biopsy specimens were evaluated for number of vessels and percentage of area occupied by vessels, using computerized image analysis after staining for type IV collagen in vessel walls. Specimens were also examined for extent of collagen III in the subepithelial basement membrane. In addition, we compared asthmatic specimens with biopsy specimens taken from non-asthmatic control subjects.Results There was a significant increase in number of vessels (P 〈 0.01) and percent vascularity (P 〈 0.001) in the submucosa of asthmatic patients compared with control subjects. After 6 months of treatment, we observed significant improvements in forced expiratory volume in 1 s (FEV1), FEV1% and airway responsiveness (P 〈 0.05, each) in the BDP treatment group compared with the placebo group. This was accompanied by significant decreases in both vessel number and percent vascularity in the airways of BDP-treated patients (P 〈 0.05, each). We also observed a significant correlation between change in percent vascularity and change in collagen III thickness in the BDP-treated patients (rs = 0.90, P 〈 0.001). Furthermore, the change in percent vascularity was inversely correlated with both FEV1 (rs = −0.49, P 〈 0.05) and airway responsiveness (rs = −0.36, P 〈 0.05).Conclusion These findings suggest that inhaled corticosteroid treatment of asthma reduced airway wall vascularity during airway remodelling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2001.01071.x
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  • 5
    Electronic Resource
    Electronic Resource
    Monoclonal antibody-detected vimentin distribution in pleomorphic adenomas of salivary glands (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 17 (1988), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To evaluate the participation of myoepithelial components in pleomorphic adenomas, an immunohistochemical study was carried out using monoclonal antibodies to vimentin. Of a total of 80 cases, 50 tumors gave positive staining, 5 tumors very slight, and 25 tumors negative staining for vimentin. Localization patterns for vimentin were divided into 3 classes: 1) vimentin staining in fibrous stromal tissue; 2) variable intensities of vimentin staining were found in the outer layers of tumor cells in tubuloductal structures (some of which were spindle cells connected to modified myoepithelial cells which also gave variable vimentin staining); and 3) modified myoepithelial cells and chondroidlike cells displayed strongly positive staining for vimentin. Typical histologic features of pleomorphic ademomas, i.e., tubulo-ductal or duct-like structures were characterized by positive vimentin staining in outer tumor cells and by a positive keratin reaction in the luminal tumor cells. In tumors devoid of stromal connective tissues and the near absence of well-developed, or modified myoepithelial cells, vimentin staining was absent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1988.tb01548.x
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  • 6
    Electronic Resource
    Electronic Resource
    Immunoreaction of keratin, actin, S-100 protein and rat-EGF in ductligated rat salivary glands (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 21 (1992), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Duct-ligated submandibular and sublingual glands of rats were evaluated immunohistochemically for changes in keratin (MoAb 1164), actin, S-100 protein and rat-EGF (rEGF). Normal salivary glands were reactive for keratin, S-100 protein and rEGF in the granular convoluted tubule (GCT) and duct cells, and for actin in the myoepithelium. Submandibular glands showed a marked reduction of S-100 protein and rEGF staining following duct ligation, and no increased staining of proliferating epithelial cells of the late stage in duct ligated glands. Sublingual glands revealed no marked changes for actin staining in myoepithelial cells, irrespective of atrophic changes occurring in acinar and duct cells after duct ligation. Immunohistochemical patterns differed for each type of gland; changes associated with the obstructive lesion were more prominent in the submandibular gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1992.tb00104.x
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  • 7
    Electronic Resource
    Electronic Resource
    Histochemical observations of lectin-binding in experimental carcinomas in mouse submandibular glands (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 13 (1984), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Peroxidase-conjugated lectins were used for the histochemical detection of carbohydrates in experimental carcinomas of mouse submandibular glands. Induced carcinomas, 43 lesions from 25 cases, were examined histoehemically with galactose-binding leetins (PNA and RCA-I), N-acetyl-galactosamine-binding leetins (DBA and SBA), a fucosc-binding lectin(UEA-l), and a N-acetyl-glucosamine-binding lectin (WGA). In non- or slightly keratinized squamous-cell carcinomas, the lectin binding of PNA, RCA-1, DBA, SBA. and WGA was weak in tumor epithelia, and UEA-1 binding was slight. In highly keratinized squamous-cell carcinomas, lectin binding was increased in tumor epithelia, but no reaction was noted in completely keratinized regions. Desquamated materials in lumens of tumors gave an intense stain with leetins. Stromal connective tissue, including collagen fibers and basement membranes stained intensely. Lectin binding to submandibular carcinomas was different from binding to granular convoluted tubules and the striated ducts of the normal submandibular gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1984.tb01460.x
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  • 8
    Electronic Resource
    Electronic Resource
    Histochemical demonstration of lectinbinding sites and keratin in inflamed human gingiva (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 20 (1985), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Histochemical demonstration of lectin-binding sites and keratin peptides in gingival epithelia was reported and differences in staining and distribution were compared to inner and outer gingival epithelia.Gingival epithelia on the outer side exhibited zonal or regional distributions of lectin-binding, and the cytochemical staining was generally found in the cell coat and intercellular materials. Keratin protein was found frequently in the spinous cell, infrequently in the basal cells, and not at all in the superficial layer. The sugar residues in the cell coat of gingival epithelia probably were mannose, galactose, N-acetyl-galactosamine, and N-acetyl-glucosamine.The inner side of the epithelia, crevicular, and pocket epithelia were characterized by irregular and incomplete staining for lectins. These epithelia also displayed less keratin staining compared to the outer gingival epithelia. These findings suggest the possibility of depolymerization of glycosaminoglycans in the cell coat by enzymes of either pocket bacterial origin or host tissue origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1985.tb00847.x
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  • 9
    Electronic Resource
    Electronic Resource
    Lectin histochemistry of ameloblastomas and odontogenic keratocysts (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 18 (1989), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The cell membrane carbohydrate components of 10 simple (follicular and/or plexiform pattern) and 5 acanthomatous ameloblastomas, one plexiform unicystic ameloblastoma, one soft tissue ameloblastoma and 11 odontogenic keratocysts were studied in paraffin-embedded tissues using horseradish peroxidase-conjugated lectins. The presence of glucose and mannose was demonstrated by intense labelling with Concanavalin ensiforme (Con A) in 73% of the ameloblastomas examined, while periodate oxidation of the specimens prior to Con A (PA/Con A) stained 53% of the cases. Ameloblastomas did not express receptors for Triticum vulgaris (WGA), Erythrina chrystagalli (ECA), Arachis hypogea (PNA), and Ulex europaeus (UEA-1). The plexiform unicystic ameloblastoma and the soft tissue ameloblastoma examined showed the same cell membrane glycoproteins as the simple and acanthomatous ameloblastomas. Forty-five per cent of the keratocysts demonstrated Con A reactivity from the basal to the keratinized layer, while 72% of these specimens showed positive PA/Con A reactivity from the parabasal to the keratinized layer. Staining with WGA, ECA, PNA, and UEA lectins also revealed the presence of N-Acetyl-glucosamine and fucose oligosaccharides in the plasma membrane of basal, spinous and keratinized cell layers of the odontogenic keratocysts. The distinct cell surface carbohydrate composition of the ameloblastoma and odontogenic keratocyst may be responsible for the differences in biological behavior in these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1989.tb00739.x
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  • 10
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of keratin proteins in duct-ligated salivary glands of mice and rats (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 15 (1986), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Immunohistochemical detection of keratin proteins in duct-ligated submandibular glands (SMG) was carried out in mice and rats with or without testosterone administration. Keratin staining in normal salivary glands was limited to the striated duct (SD) and excretory duct (ED) cells and was usually lacking in granular convoluted tubule (GCT) cells. Following duct-ligation, the epithelia of intercalated ducts (ICD), degranulated tubules, duct-like structures, and dilated striated and excretory ducts showed positive keratin staining, usually in their luminal aspects. The concentration of keratin was proportional to the degree of degranulation of the GCT cells. The duct-ligated SMGs in animals with testosterone treatment showed a comparatively higher number of granules located in the GCT cells, and degranulation was slight. Keratin staining in hormone-treated duct-ligated glands also occurred in ductal segments to a slight degree. Keratin was also detected in degranulated tubules, and its concentration was increased in duct-like structures, whereas staining for EGF and NGF was decreased in degranulated tubules and lacking in duct-like structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1986.tb00558.x
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