ISSN:
1365-3040
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract. Survival and death of Chara internodal cells were investigated in one of the alkali metal salts KCl, some of the alkali earth metal salts CaCl2, Ca(NO3)2, MgCl2, Mg(NO3)2, SrCl2, Sr(NO3)2, BaCl2 and Ba(NO3)2, potassium phosphate pH buffer solution (pH 7.0), Tris-maleate pH buffer solution (pH 7.0), HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid)-KOH (pH 7.0) pH buffer solution, calcium buffer solutions, and deionized water. Most of the internodal cells died within a day or a few days in KCl, MgCl2, Mg(NO3)2, BaCl2 and Ba(NO3)2 solutions of higher concentrations, calcium buffer solutions of pCa 6.0, 10.0 mol m-3 potassium phosphate pH buffer solution and 10.0 mol m-3 Trismaleate pH buffer solution. However, all of the internodal cells survived more than 10 d in deionized water, 80.0 mol m-3 CaCl2, 80.0 mol m-3 Ca(NO3)2, 80.0 mol m-3 SrCl2, 80.0 mol m-3 Sr(NO3)2 calcium buffer solutions of pCa 4.0 and pCa 5.0, and 10.0 mol m-3 HEPES-KOH (pH 7.0) pH buffer solution. Addition of Ca2+ or Sr2+ to K+, Mg2+ and Ba2+ salt solutions increased the survival rates of the internodal cells. Calcium release from the internodal cell wall was measured in deionized water, KCl, NaCl, MgCl2, CaCl2, SrCl2 and BaCl2 solutions. Except in deionized water and CaCl2 solution, most of the calcium binding to the cell wall was released within one or a few hours in respective electrolyte solutions. Thus, survival and death of the internodal cells in the electrolyte solutions tested were interpreted in terms of the calcium release from the cell wall and the cell membrane, and intrinsic ability of Sr2+ to maintain the cell membrane normal.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3040.1990.tb01324.x
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