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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Grass and forage science 59 (2004), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of changes from conventional grassland management on fenland in Germany to extensification due to reduced fertilizer inputs and cutting frequency on the microbial colonization in the phyllosphere were investigated. A delay in the first cut for silage and hay, required by a nature conservation programme, was accompanied by the senescence of grasses which significantly influenced certain microbial groups. Heterotrophic bacteria, aerobic and anaerobic spore-formers, as well as filamentous fungi, showed an increase in population densities on the mature herbage of the later cuts. Differences in the rate of fertilizer application and cutting frequency, which had an effect on the dry-matter yield, also influenced the population density of heterotrophic bacteria and, in certain circumstances, that of filamentous fungi. Chemical measures of forage quality were highly correlated with the population density of microbial groups and with the maturity of the herbage. In contrast to these results, the population dynamics of further groups of micro-organisms (yeasts, Micrococcaceae, listeria) did not show any relationship with pasture management.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: GFR measurement ; bolus technique ; conscious dogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The renal clearance of creatinine was measured following orogastric administration in conscious dogs. Values of creatinine clearance were compared with simul taneously determined values of inulin clearance, when urine volume, glomerular filtration rate and volume status were acutely altered by a variety of experimental manoeuvres. At urine volumes greater than 20 μl. min−1 · kg−1, creatinine clearance was not significantly different from inulin clearance. At low urine volumes there was some evidence of creatinine reabsorption. It is concluded that the bolus creatinine technique provides reliable estimates of glomerular filtration rate and is particularly applicable to long-term studies in conscious dogs.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 6 (1991), S. 123-125 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple high density cell culture system is described which demonstrates many of the features of commercially available hollow-fibre systems, but without the need to invest in a dedicated system. The system has been shown to achieve product concentrations of up to 40-fold greater than that obtained in batch culture making gram production of MAbs possible with considerable saving on serum costs.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 308-314 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The cultural conditions for the production of enhanced formation of L-serine (up to 7 g/L) are described with the methylotrophic bacterium Pseudomonas 3 ab (DSM 672). The batch process is divided into three parts: (1) the biomass production phase, (2) substrate limitation period, and (3) L-serine accumulation phase. The initial specific production rate of qp = 0.1 g L-serine/g dry wt/h is based on the inhibition of the L-serine pathway. This is accomplished by high precursor concentrations (glycine) and a pH shift to pH 8.5. The enzymatic background is discussed. Furthermore, a coupling of the L-serine process with a L-tryptophan-producing process is demonstrated.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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