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  • 1
    Electronic Resource
    Electronic Resource
    Excision-repair Properties of an Escherichia coli Mutant deficient in DNA Polymerase (1970)
    [s.l.] : Nature Publishing Group
    Nature 226 (1970), S. 708-710 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] An Escherichia coli mutant, extracts of which show no DNA polymerase activity, can excise pyrimidine dimers induced by ultraviolet irradiation. The increased radiation sensitivity of this strain is related to the extensive degradation of irradiated ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/226708a0
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  • 2
    Electronic Resource
    Electronic Resource
    Thymine dimer repair in fibroblasts of patients with dysplastic naevus syndrome (DNS) (1988)
    Springer
    Cellular and molecular life sciences 44 (1988), S. 169-171 
    Details
    ISSN: 1420-9071
    Keywords: Dysplastic naevus syndrome ; DNA-repair ; cancer genes ; familial malignant melanoma ; monoclonal antibodies specific for UV-dimers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dysplastic naevus syndrome (DNS) is frequently observed in association with familial melanoma and xeroderma pigmentosum (XP), but the role of UV-light in the development of DNS has not been elucidated. Previous work has shown that UV-induced unscheduled DNA synthesis is associated with the early loss of antigenicity observed in immunoassays using a monoclonal antibody specific for thymine-thymine dimers. We now show that the rate of loss of antigenicity, which reflects the relative amount of bound antibody, observed during the first 60 min following 10 Jm−2 UVC irradiation is significantly reduced (p=0.02) in cultures of fibroblasts from 7 out of 8 DNS patients compared with the results from cells of a group of 30 healthy volunteers. This observation suggests an early event in excision repair is altered in the majority of DNS patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952205
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  • 3
    Electronic Resource
    Electronic Resource
    Regional localization of human ecto-5′nucleotidase to chromosome 6q14–q21 (1989)
    Springer
    Human genetics 〈Berlin〉 83 (1989), S. 179-180 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human and mouse hybrids that contain fragments of human chromosome 6 as translocations were analysed for expression of ecto-5′nucleotidase enzymic activity measured by the conversion of AMP to adenosine and for antigenicity recognized by a monoclonal antibody specific for the human isozyme. Both methods allow a regional assignment of ecto-5′nucleotidase to 6q14–q21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286714
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  • 4
    Electronic Resource
    Electronic Resource
    Trophoblast glycoprotein recognised by monoclonal antibody 5T4 maps to human chromosome 6q14–q15 (1990)
    Springer
    Human genetics 〈Berlin〉 84 (1990), S. 455-458 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human × rodent hybrids were stained by indirect immunofluorescence with 5T4, a murine monoclonal antibody that recognises a 72 kdalton glycoprotein expressed by human trophoblasts and a very restricted range of adult tissues; they were analysed by flow cytometry. Concordance analysis supported by segregation data allowed assignment of the gene controlling glycoprotein expression (M6P1) to chromosome 6. Similar analysis with translocation hybrids gave a regional assignment to 6q14–q15. M6P1 is distinct from NT5, coding for 5′ nucleotidase, which maps to the same region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195819
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  • 5
    Electronic Resource
    Electronic Resource
    Assignment of ecto-5′-nucleotidase to human chromosome 6 (1988)
    Springer
    Human genetics 〈Berlin〉 81 (1988), S. 88-92 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ecto-5′-nucleotidase activity (5NT) was measured on whole cells of 26 human x Chinese hamster hybrids. Concordance analysis showed 100% correlation between enzyme activity and inheritance of human chromosome 6. This observation was confirmed by a segregation analysis in which cells of a hybrid containing chromosome 6 were stained by indirect immunofluorescence for HLA Class 1 antigen and sorted by a fluorescence-activated cell sorter (FACS). Cells in the HLA- compartment were cloned and expression of HLA and 5NT was determined. Of nine clones, three were HLA-, 5NT- and six were HLA+, 5NT+, supporting the linkage of 5NT to chromosome 6.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283737
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  • 6
    Electronic Resource
    Electronic Resource
    Nucleotide ectoenzyme activities of human and Chinese hamster fibroblasts in tissue culture (1989)
    Springer
    Biochemical genetics 27 (1989), S. 655-671 
    Details
    ISSN: 1573-4927
    Keywords: 5′-nucleotidase ; biochemical genetics ; purine salvage ; ectoenzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We have previously assigned human ecto-5′-nucleotidase (NT) to chromosome 6 on the basis of conversion of exogenously supplied [14C] AMP to adenosine by whole cells of human and Chinese hamster hybrids carrying chromosome 6. In this paper we demonstrate that the activity on human MRC-5 fibroblasts is typical of previously described and purified ecto-5′-nucleotidases. In contrast to MRC-5 cells, Chinese hamster V79A2 cells weakly express an AMPase activity that is not NT. The cytosolic form of NT in human and hybrid fibroblasts is similar to the ectoenzyme in substrate specificity. Hybrids that lack chromosome 6 express neither the ecto- nor the cytosolic enzyme, suggesting that both forms may be coded by the same gene on chromosome 6. Ecto-ATPase, ecto-ADPase, and ecto-ADP kinase activities are each expressed at similar levels in MRC-5 and V79A2. The ATPase, ADPase and NT activities of MRC-5 cells act sequentially to generate adenosine. A similar cascade acts on V79A2 cells but the lack of NT causes the accumulation of AMP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02396058
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleotide ectoenzyme activities of human and Chinese hamster fibroblasts in tissue culture (1989)
    Springer
    Biochemical genetics 27 (1989), S. 655-671 
    Details
    ISSN: 1573-4927
    Keywords: 5′-nucleotidase ; biochemical genetics ; purine salvage ; ectoenzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We have previously assigned human ecto-5′-nucleotidase (NT) to chromosome 6 on the basis of conversion of exogenously supplied [14C] AMP to adenosine by whole cells of human and Chinese hamster hybrids carrying chromosome 6. In this paper we demonstrate that the activity on human MRC-5 fibroblasts is typical of previously described and purified ecto-5′-nucleotidases. In contrast to MRC-5 cells, Chinese hamster V79A2 cells weakly express an AMPase activity that is not NT. The cytosolic form of NT in human and hybrid fibroblasts is similar to the ectoenzyme in substrate specificity. Hybrids that lack chromosome 6 express neither the ecto- nor the cytosolic enzyme, suggesting that both forms may be coded by the same gene on chromosome 6. Ecto-ATPase, ecto-ADPase, and ecto-ADP kinase activities are each expressed at similar levels in MRC-5 and V79A2. The ATPase, ADPase and NT activities of MRC-5 cells act sequentially to generate adenosine. A similar cascade acts on V79A2 cells but the lack of NT causes the accumulation of AMP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553987
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  • 8
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of the glucose-transport protein in mammalian brain capillaries (1989)
    Springer
    Journal of molecular histology 21 (1989), S. 47-51 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The endothelial cells of mammalian brain capillaries, which form the anatomical basis of the blood-brain barrier, have been investigated by immunocytochemical methods to determine the distribution of the glucose-transport protein. A monoclonal antibody raised against the intact human erythrocyte glucose-transport protein and polyclonal antibodies raised against a synthetic peptide corresponding to the C-terminal sequence of the human erythrocyte glucose-transport protein were used for immunofluorescent staining of isolated human and bovine cerebral cortex microvessels. The pattern of fluorescence with both antibodies demonstrated the antigen to the distributed throughout the plasma membrane of the capillary endothelial cells. These results provide further evidence for the homology between the human erythrocyte and brain capillary glucose-transport protein, and confirm its abundance in brain capillaries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002471
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of chromosome 6 deletions in lymphoid malignancies provides evidence for a region of minimal deletion within a 2-megabase segment of 6q21 (1997)
    Springer
    Chromosome research 5 (1997), S. 118-124 
    Details
    ISSN: 1573-6849
    Keywords: chromosome 6 ; deletion ; lymphoid malignancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescence in situ hybridization has been used to define deletion breakpoints within chromosome bands 6q16–21 incases of lymphoid malignancy. Previous evidence suggested that the region might contain a tumour-suppressor gene. Six yeast artificial chromosome probes, each selected using a single marker, were localized to 6q16–21 and the following order was confirmed; D6S330–D6S283–D6S301–D6S447–D6S246–FYN. Of 32 cases of lymphoid malignancy, 30 showed deletion of D6S246 and, in the two cases in which D6S246 was retained, the adjacent marker, D6S447, was deleted. These observation simply that a region of minimal deletion is located within a 2-megabase segment of 6q21, between D6S447 and D6S246, providing a candidate region for the location of a tumour-suppressor gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018418224660
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  • 10
    Electronic Resource
    Electronic Resource
    A radiation hybrid panel for human Chromosome 6q (1995)
    Springer
    Mammalian genome 6 (1995), S. 285-290 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A panel of 63 radiation-reduced hybrids has been derived from a mouse cell line containing a neo-marked human Chromosome (Chr) 6, primarily to provide a resource for higher resolution localization of new markers. Hybrids were generated with radiation doses of 40–400 Gy, selected in G418, and were shown by PCR to contain the neo gene. PCR was also used to score the retention of 15 loci that map from 6q13 to q25.2 of the current consensus map plus six other loci assigned to 6q26-q27. An average retention frequency of 27.8% was observed, with the highest frequencies at D6S313 and D6S280 (63.5%) located near the centromere at 6q13, and at D6S283 (68.5%) at 6q16.3-q21, presumably close to the neo integration site. Lowest frequencies (4.8%) were observed for telomeric markers. All markers segregated independently except D6S297 and D6S193. Agreement and some improvement to the current consensus map of 6q was made by mapping 12 loci by the non-parametric statistical method of Falk. In addition, deletion mapping with informative hybrids allowed the ordering of six loci from 6q26 to q27 and permitted some integration of maps of this region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352418
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