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  • 1
    Electronic Resource
    Electronic Resource
    Integrated sensor systems for environmental monitoring (1997)
    Bradford : Emerald
    Sensor review 17 (1997), S. 327-333 
    Details
    ISSN: 0260-2288
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Discusses the requirements in environmental monitoring - primarily in aqueous environments. Detection of substances below the ppb-level may be required. Many analytes lack intrinsic properties suitable for direct detection by a sensor. Therefore, separation, concentration, and chemical conversion may be required. Sensors may require calibration and conditioning. Efforts are taken to integrate these functions with sensor components to give integrated sensor systems, which allow unattended operation in the environment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/02602289710185171
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein β-adaptin (1991)
    [s.l.] : Nature Publishing Group
    Nature 349 (1991), S. 215-220 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Four high-molecular-weight proteins form the main subunits of the coat of Golgi-derived (non-clathrin) coated vesicles. One of these coat proteins, β-COP, is identical to a Golgi-associated protein of relative mass 110,000 (110K) that shares homology with the adaptin proteins of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/349215a0
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Label free binding assay with spectroscopic detection for pharmaceutical screening (1997)
    Springer
    Fresenius' journal of analytical chemistry 359 (1997), S. 15-22 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Many therapeutic drugs exert their effects by interaction with well defined molecular targets. Increasing knowledge in molecular biology allows identification of more and more molecular key compounds and in consequence a molecular approach to disease and therapy. As binding of drugs to their target compounds is a key event, binding assays with an appropriate target molecule are useful means for primary screening of novel substances. We have investigated the potential of thin film interference spectroscopy (RIFS) as a label free detection method for pharmaceutical screening in a binding inhibition assay. To meet the throughput requirements in pharmaceutical screening a parallel detection system based on imaging spectroscopy was constructed. Thrombin/thrombin inhibitor interaction was investigated as a model system. The thin film transducer was covalently modified with a thrombin inhibitor. Specific binding of thrombin and binding inhibition by inhibitor compounds could be observed. A test cycle of less than 10 min could be reached. The parallel setup allows the simultaneous detection of 96 binding curves and can reach a throughput of more than 106 samples per year.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050529
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Mechanisms of Fluorescence Quenching in Donor—Acceptor Labeled Antibody—Antigen Conjugates (2000)
    Springer
    Journal of fluorescence 10 (2000), S. 147-147 
    Details
    ISSN: 1573-4994
    Keywords: Cyanine dyes ; energy transfer ; electron transfer ; Poisson distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The cyanine dyes Cy5 and Cy5.5 are presented as a new long wavelength-excitable donor-acceptor dye pair for homogeneous fluoroimmunoassays. The deactivation pathways responsible for the quenching of the fluorescence of the antibody-bound donor are elucidated. Upon binding of the donor dye to the antibodies at low dye/protein ratios, its fluorescence quantum yield rises to unity. Higher dye/protein ratios lead to progressive aggregation of the dyes, which results in quenching of monomer fluorescence due to resonance energy transfer (RET) from the monomers to the nonfluorescent dimers. The dependence of the quenching efficiency on the labeling ratio is described quantitatively by assuming a Poisson distribution of the dyes over the antibodies. The maximum fluorescence intensity per antibody is obtained at a labeling ratio of 4. Upon formation of the antibody-antigen complex, electron transfer and RET to the antigen-bound acceptor dye occur. Steady-state and time-resolved fluorescence measurements reveal that approximately 50% of the donor quenching is due to RET, while the residual quenching effect is caused by the static quenching process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009443125878
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Characterisation and optimisation of an immunoprobe for triazines (1996)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 354 (1996), S. 857-860 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The characterisation and optimisation of an optical immunoassay with label free detection based on Reflectometric Interference Spectroscopy (RIfS) is presented. The immunoprobe is operated in a sequential scheme, where Fab-fragments react with analyte molecules in a first step. In a second step the optical transducer is used to quantify the amount of unoccupied Fab- fragments in the reaction mixture binding to the hapten-modified transducer surface. For optimisation of the test, the Fab-fragment concentration was varied between 2×10-8 mol/l and 2.5× 10−9 mol/l. Down to a concentration of 5×10-9 mol/l a reduction in the limit of detection has been observed. At the lowest concentration investigated no further improvement has been found due to a reduced binding of the analyte and a strong decrease of antibody binding at the transducer surface. This finding could be explained by the thermodynamics of the antigen-antibody reaction and the performance of the optical transducer used. The limit of detection obtained is discussed with respect to thermodynamics, transducer characteristics and immunoprobe test format.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s0021663540857
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    Paper (German National Licenses)
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