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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 165 (1985), S. 142-144 
    ISSN: 1432-2048
    Keywords: Androgenesis ; Anther ; Citrus ; Embryoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryoids were differentiated from anthers of C. aurantifolia which were first floated on a modified Murashige and Skoog's liquid medium supplemented with 0.5 mg/l N6-benzylaminopurine and 1 mg/l indole-3-acetic acid for 20–30 d, followed by 30 d culture in semisolid Schenk and Hildebrandt's medium having the same growth hormones. Embryoids originated from within the anther lobes. Initially, a few embryoids were formed by each anther; later, they multiplied rapidly by the production of new embryoids from the hypocotyl and cotyledon portions of the original embryoids. The embryoids could develop into plantlets, which were all diploid (2n=18). The androgenic plants grew normally in soil.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 1 (1981), S. 33-38 
    ISSN: 1573-5044
    Keywords: Bougainvillea ; clonal propagation ; shoot apex culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot apices ofBougainvillea glabra ‘Magnifica’ were induced to regenerate an average of ten shoots from their bases in response to BAP (0.5 mg/l) plus IAA (1.5 mg/l). All the isolated shoots from such cultures were rooted in a medium containing 0.1 mg/l each of IBA and 2,4,5-T and lacking BAP. Plantlets were then successfully grown in potted soil where they flowered normally.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 3 (1984), S. 163-168 
    ISSN: 1573-5044
    Keywords: Rosmarinus officinalis ; clonal propagation ; shoot proliferation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Single-node stem segments of Rosmarinus officinalis L. var. genuina forma erectus proved better explants than shoot tips (ca. 2 cm long) for extablishment of field-grown plants in aseptic cultures. Benzylaminopurine was far more effective than kinetin for shoot induction in shoot tips excised from aseptically-grown plants. Maximum numbers of shoot buds (ca. 14) were formed per explant at 0.2 mgl-1 benzylaminopurine in 30 days. After further growth of isolated shoots and treatment with 0.25 mgl-1 indolepropionic acid for 7 days, 80% shoots produced roots. In vitro raised plantlets were successfully grown in soil to plants. About 5,000 plants could be produced from a single nodal segment in 1 year.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 38 (1994), S. 73-75 
    ISSN: 1573-5044
    Keywords: gibberellic acid ; morphogenic potential ; retrieved shoots ; undifferentiated callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regeneration potential was restored in 2-year-old cultures of proliferating shoots of Digitalis purpurea L. under the influence of 14.43 μM gibberellic acid, while cytokinins were ineffective. The retrieved shoots grew normally in a modified Murashige and Skoog's medium supplemented with 0.98 μM indole-3-butyric acid (IBA) and 27.1 μM adenine sulphate and rooted 100% in the presence of 0.49 μM IBA alone.
    Type of Medium: Electronic Resource
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