ISSN:
1432-2048
Keywords:
Androgenesis
;
Anther
;
Citrus
;
Embryoid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Embryoids were differentiated from anthers of C. aurantifolia which were first floated on a modified Murashige and Skoog's liquid medium supplemented with 0.5 mg/l N6-benzylaminopurine and 1 mg/l indole-3-acetic acid for 20–30 d, followed by 30 d culture in semisolid Schenk and Hildebrandt's medium having the same growth hormones. Embryoids originated from within the anther lobes. Initially, a few embryoids were formed by each anther; later, they multiplied rapidly by the production of new embryoids from the hypocotyl and cotyledon portions of the original embryoids. The embryoids could develop into plantlets, which were all diploid (2n=18). The androgenic plants grew normally in soil.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392223
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