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  • 1
    Electronic Resource
    Electronic Resource
    Recombination between the linear plasmid pPZG101 and the linear chromosome of Streptomyces rimosus can lead to exchange of ends (1998)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 28 (1998), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The 387 kb linear plasmid pPZG101 of Streptomyces rimosus R6 can integrate into the chromosome or form a prime plasmid carrying the oxytetracycline biosynthesis cluster. The integration of plasmid pPZG101 into the linear chromosome of S. rimosus R6-501 in mutant MV25 was shown to be due to a single cross-over at a 4 bp common sequence. pPZG101 had integrated into a 250 kb DNA sequence that was reiterated at a low level. This sequence includes the oxytetracycline biosynthesis cluster, so that homologous recombination generated a mixed population carrying different copy numbers of the region. The 1 Mb linear plasmid pPZG103 in mutant MV17 had also arisen from a cross-over between pPZG101 and the chromosome, so that one end of pPZG103 consists of c. 850 kb of chromosomal sequence including the oxytetracycline biosynthesis cluster. The plasmid pPZG101 was shown to consist of a unique central region of about 30 kb flanked by terminal inverted repeats of about 180 kb. Analysis of a presumed ancestor plasmid pPZG102 suggested that the long terminal repeats had arisen by a recombination event during the strain development programme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1998.00877.x
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  • 2
    Electronic Resource
    Electronic Resource
    Stabilization of Streptomyces lividans by Homologous Recombinational Insertion (1992)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 10 (1992), S. 570-573 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have developed a system for the introduction and maintenance of novel tandem repeats in the chromosome of Streptomyces lividans 66. This was achieved by introducing, via transformation, Escherichia coli “suicide” vectors carrying manipulated S. lividans DNA fragments. Selection for ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0592-570
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  • 3
    Electronic Resource
    Electronic Resource
    Amplification of Cloned Genes in Streptomyces (1987)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 5 (1987), S. 1328-1329 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A thiostrepton resistance gene and a tyrosinase gene were linked to an amplifiable DNA sequence from Streptomyces lividans 66. This construct was introduced by transformation into S. lividans where it integrated into the chromosome by homologous recombination. When spontaneous DNA amplification was ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1287-1328
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  • 4
    Electronic Resource
    Electronic Resource
    The pyrroloquinoline quinone synthesis genes of Gluconobacter oxydans (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 193 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A Tn5-induced glucose dehydrogenase (GDH) deficient mutant of Gluconobacter oxydans IFO 3293 was characterised. DNA sequencing showed that the insertion site occurred in an open reading frame with homology to the pqqE gene. It was shown that acid production could be restored by addition of the coenzyme pyrroloquinoline quinone (PQQ) to the medium. The pqq cluster of G. oxydans ATCC 9937 was cloned and sequenced. It has five genes pqqA–E. The cluster could complement the Tn5-induced mutation in IFO 3293. Pulsed-field gel electrophoresis suggested that the pqq genes are not closely linked to the ribF gene that produces the riboflavin cofactor for the gluconic acid dehydrogenase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09429.x
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  • 5
    Electronic Resource
    Electronic Resource
    A mutant of Gluconobacter oxydans deficient in gluconic acid dehydrogenase (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 179 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Gluconobacter oxydans ATCC 9937 was subjected to transposon mutagenesis using Tn5. A non-pigmented mutant was shown to be defective in gluconic acid dehydrogenase and to produce gluconic acid from glucose, whereas the parent strain produced 2,5-diketogluconic acid. Cloning and sequencing of the region containing the Tn5 insertion showed that the insertion point occurred in an open reading frame homologous (42% amino acid identity) to the ribF genes of Pseudomonas fluorescens and Escherichia coli. The resulting lack of a riboflavin cofactor would explain the loss of enzyme activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb08769.x
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  • 6
    Electronic Resource
    Electronic Resource
    Dispersed growth of Streptomyces in liquid culture (1989)
    Springer
    Applied microbiology and biotechnology 31 (1989), S. 272-277 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The study of the physiology of the filamentous bacterium Streptomyces is inhibited by its formation of mycelial pellets in liquid cultures. It is demonstrated that dispersed growth may be achieved by the addition of polymers to the culture medium. Uncharged polymers, such as polyethylene glycol, are relatively ineffective but polyanions such as agar, Carbopol and Junlon produce dispersed cultures when included in a defined growth medium at low concentrations. Junlon-containing media enable optical density measurements to be used to follow batch growth of Streptomyces. Improvements in both biomass yield and product yield of the pigmented antibiotic actinorhodin were found to result from the incorporation of Junlon into minimal medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00258408
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  • 7
    Electronic Resource
    Electronic Resource
    Conjugational transfer systems of Rhodopseudomonas capsulata mediated by R plasmids (1981)
    Springer
    Archives of microbiology 128 (1981), S. 390-393 
    Details
    ISSN: 1432-072X
    Keywords: Conjugational transfer ; R plasmids ; Rhodopseudomonas capsulata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasmids RP1, R68.45 and RP4::Mu cts 61 were transferred into Rhodopseudomonas capsulata from Escherichia coli. The frequency of intraspecies transfer of these plasmids in R. capsulata was 10-4–10-5 per donor. The plasmids also mobilized chromosomal genes at a low frequency. Phototrophic recombinants from matings between recipient strains defective in the photosynthetic-apparatus and wild type donors were obtained at a frequency of 10-7–10-8 per donor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405918
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  • 8
    Electronic Resource
    Electronic Resource
    IS2-43 and IS2-44: New alleles of the insertion sequence IS2 which have promoter activity (1979)
    Springer
    Molecular genetics and genomics 175 (1979), S. 53-56 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sequence of two new IS2 alleles with promoter activity (IS2-43 and IS2-44) is reported. The alleles are identical and are formed by a 17 bp tandem duplication in an AT-rich region of IS2. This created a new RNA polymerase binding site. A mutation was found that increased the frequency of formation of these 17 bp duplications but not of another class of duplications, the “mini-insertions”. This suggested that the mechanisms of formation of the two classes of duplications are different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267855
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  • 9
    Electronic Resource
    Electronic Resource
    DNA deletions in spontaneous chloramphenicol-sensitive mutants of Streptomyces coelicolor A3(2) and Streptomyces lividans 66 (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 499-502 
    Details
    ISSN: 1617-4623
    Keywords: Unstable gene ; DNA amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A mutant of Streptomyces coelicolor A3(2) highly resistant to chloramphenicol was selected. It had amplified some chromosomal DNA fragments to a copy number of 20–50. Some of the amplified fragments were cloned and used as hybridisation probes to investigate the spontaneous chloramphenicol-sensitive mutants which occur at high frequency in this species and the closely related species Streptomyces lividans 66. These investigations demonstrated that chloramphenicol sensitivity in both species is associated with large deletions that are at least 40 kb in length.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331621
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  • 10
    Electronic Resource
    Electronic Resource
    Structure of an amplifiable DNA sequence in Streptomyces lividans 66 (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 192-197 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spontaneous chloramphenicol-sensitive mutants of Streptomyces lividans 66 had previously been shown to be very unstable and to yield arginine auxotrophic mutants at a frequency of 25% of spores; the Arg- mutants had amplified a particular 5.7 kb DNA sequence to over one hundred tandem copies per genome. In this paper we report the cloning of the amplifiable region from amplified and wild-type strains. This showed that the amplifiable fragment is already present as a duplication in wild type cells. Hybridisation experiments also demonstrated that in the amplified strains there was a deletion of neighbouring DNA sequences to one side of the amplifiable element; sequences to the other side remain intact.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425659
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