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  • 1
    Electronic Resource
    Electronic Resource
    Co-culture of neurones with Merkel cells and keratinocytes (2004)
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc
    Experimental dermatology 13 (2004), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In order to provide a model for in vitro studies of the interactions between skin and the nervous system, we have performed a co-culture of epidermal cells and neurons. We used a tri-compartmented box with separated domains. In the central part of this box, we put an epidermal suspension (4 millions cells/ml) obtained from biopsies of human skin. Around this central domain, we disposed sensorial neurons from the dorsal root ganglia of rats. The periphery was occupied by neurons from the dorsal horn of spinal cord. Sensorial neurons grew in low density (500 cells), on a glial layer, in a medium conditioned by astrocytes. After 15 days of culture, cells were fixed and stained with monoclonal antibodies directed against PGP 9.5, keratins, or cytokeratin 20 (Merkel cells). We obtained a co-culture with three identifiable territories, equivalents of epidermis, root ganglia, and spinal cord. Nervous fibers specifically grew from the sensorial neurons to epidermal cells or to the spinal cord equivalent. We observed synapse-like contacts between nerve endings and Merkel cells or keratinocytes. This model allows us to reconstruct in vitro an equivalent of sensitive nerve fibers, connected for one part to a spinal cord equivalent and on the other part to an epidermis equivalent. Such a model could be used to understand the origin and the function of Merkel cells in the epidermis and to study synapses in the skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0906-6705.2004.0212as.x
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  • 2
    Electronic Resource
    Electronic Resource
    The ageing phenomenon of turbot spermatozoa: effects on morphology, motility and concentration, intracellular ATP content, fertilization, and storage capacities (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 52 (1998), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The deleterious effect of the ageing phenomenon of turbot spermatozoa was investigated in relation to the sampling date. Spermatozoa with a low or highly condensed chromatin and a middle piece containing numerous or a few vesicles were observed simultaneously 80 and 47 days before the beginning of the spawning period of the females. The middle piece of spermatozoa contained few vesicles, 39 days after the end of the reproductive period. At the same date, some spermatozoa appeared in which the plasma membrane was broken. Sperm motility, assessed just after collection in terms of arbitrary motility scores from 0 to 5, was significantly increased both at 10 and 60 s post-activation, for samples collected 18 days after, 25 days before and 9 days after the beginning of the spawning period of the females, respectively compared to samples collected 6 days before, 55 days after and 88 days after the end of this period. A lower short-term storage capacity was recorded at 10 and 60 s post-activation for sperm samples collected 6 days before and 88 days after the end of the reproductive period, respectively compared to 18 days and 9 days after the beginning of the spawning period. At 60 s post-activation, a higher motility of thawed spermatozoa was observed for samples collected 5 days before the beginning of the spawning period (motility recovery index: 86.4 ± 19.4%) compared to 71 days after the end of this period (55.0 ± 12.0%). The fertilizing capacity of sperm samples collected 61 days after the end of the spawning season (66.1 ± 14.6%) was significantly lower than that recorded for samples collected 34 days after the beginning of the spawning period (75.2±9.6%). On the contrary, there was no significant decrease in endogenous ATP content (31 days after the beginning of the spawning period, 14.53 ± 0.84; 48 days after the end of this period, 10.75 ± 5.26 nmol 10− 8 spermatozoa). Furthermore, sperm concentration significantly increased between the same dates (respectively 3.3 ± 0.8–9.4±4.8×109 spermatozoa ml−1).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1998.tb01550.x
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  • 3
    Electronic Resource
    Electronic Resource
    Composition of the seminal Fluid and ultrastructure of the spermatozoon of turbot (Scophthalmus maximus) (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 42 (1993), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mean values of the composition of the seminal fluid of 30 turbots were: osmotic pressure =306 mosml−1, pH =7–31, total protein =8.8mg ml1, Na+=133.0 mmoll1, K+=3.80mmoll−1, Cl−=129mmol l−1. The rounded nucleus, the reduced middle piece and the typical ‘9 + 2’ structure of the flagellum mean that the spermatozoon of turbot can be considered to be of a primitive type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1993.tb00355.x
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  • 4
    Electronic Resource
    Electronic Resource
    A technique for separating high- and low-quality embryos of the scallop, Pecten maximus L.
    Amsterdam : Elsevier
    Aquaculture 120 (1994), S. 341-346 
    Details
    ISSN: 0044-8486
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0044-8486(94)90090-6
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  • 5
    Electronic Resource
    Electronic Resource
    Utrastructural study of oogenesis and oocytic degeneration in Pecten maximus from the Bay of St. Brieuc (1989)
    Springer
    Marine biology 103 (1989), S. 339-348 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An ultrastructural study of oocytic development enabled the identification of changes occurring during oogenesis in Pecten maximus collected from the Bay of St. Brieuc, France, in 1987. “Auxiliary cells”, closely associated with developing oocytes were observed. Each oocyte seems to be associated with only one secretory cell, which is characterised by an abundant rough endoplasmic reticulum at the onset of vitellogenesis. Contact between this cell and a developing oocyte is maintained by a desmosome-like junction which can be observed when the vitelline coat is formed. These “auxiliary cells” seem to play a trophic role in vitellogenesis, and may be involved in the formation of the vitelline coat of the oocytes. Oocytic degeneration is discussed in detail; in this species, it is a continuous phenomenon of varying intensity throughout the year. The ultrastructural changes resulting in lysis of the oocyte are described, and the evolution of atretic oocytes is examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397268
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  • 6
    Electronic Resource
    Electronic Resource
    Recovery of all species from photolytic degradation of tributyltin compounds TBTX (X = Cl, OSn Bu3) (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 1 (1987), S. 133-142 
    Details
    ISSN: 0268-2605
    Keywords: Photolytic degradation ; Kinetics ; Bis(tributyltin)oxide ; Tributyltin chloride ; Antifouling paints ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Malformations in shellfish have been reported by many authors. They attributed the cause of the deformity to the presence in water of organotin compounds used in the formulation of antifouling paints, for example bis(tributyltin) oxide (TBTO) and tributyltin chloride (TBTC). The behaviour of these compounds has been examined under abiotic laboratory conditions. The influence of many parameters such as sunlight, pH, oxygen, salinity have been examined. The degradation compounds obtained have been identified: (1) In the gas phase two major products, butene-1 and buetene-2, are observed with consumption of oxygen; (2) In the liquid phase, three main products are obtained, butanol-1, butanol-2 and butanone 2. The identified products represent a small part of the total concentration, suggesting a competing process such as formation of butyltin polymers; (3) In fresh water an amorphous solid phase is observed while in seawater a white cristalline precipitate appears.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aoc.590010205
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