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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 178 (1956), S. 307-308 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] This amount of ribonucleic acid consists of about 6,000 nucleotides. However, this should not be interpreted to be a single macromolecule comparable to some sort of gene. It has been shown in some strains of plant and animal viruses that there are smaller sub-units containing the total ribonucleic ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    BBA - Biochimica et Biophysica Acta 26 (1957), S. 40-46 
    ISSN: 0006-3002
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 82 (1951), S. 755-758 
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 82 (1951), S. 758-760 
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 81 (1950), S. 607-609 
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The purification of anti-BPO-antibodies and anti-BPO-IgG resp. by means of affinity chromatography is being described. The immunosorbent was prepared by direct covalent coupling of BPO groups to AH-Sepharose 4B beads. The antibodies were linked to the immunosorbent in a batch and eluted by stepwise chromatography. The hapten density of the gel was very high (3.6×10−5 mole BPO/1 g Sepharose 4B) as could be demonstrated by application of14C-penicillin-potassium. Good yields of highly purified anti-PBO-antibodies were obtained.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1076
    Keywords: Hepatitis B: prevention and control ; Vaccination ; Paediatrics ; Medical oncology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Fifty children with malignant diseases were vaccinated against hepatitis B. Twenty-nine children suffered from leukaemia or non-Hodgkin's lymphoma; 14 of these were on intensive chemotherapy (group I) and 15 were without intensive therapy (group II). The other 21 children had various forms of solid tumours, 14 of them were on intensive therapy (group III) and 7 were without intensive therapy (group IV). To evaluate the immune response, we determined antibody titres over a period of more than 14 weeks after the first vaccination. As 22 out of 50 patients had received passive immunisation together with either the first or the first and second vaccination, antibody titres at the 14th and 18th week (i.e. more than 10 weeks after passive immunisation) were used to evaluate the vaccination results. An antibody titre of ≥10 mIU/ml was considered to be a positive response. All patients of group IV, but only 4 out of 14 in group III, 4 out of 15 in group II, and 0 out of 14 in group I produced antibody titres higher than 50 mIU/ml. In contrast to the full response in group IV, two-thirds of all other patients had no immune response (〈10 mIU/ml). Based on our experience we recommend vaccinating patients suffering from solid tumours and receiving no intensive therapy (group IV) against hepatitis B and protecting all the other children with malignant diseases by passive immunisation, if necessary.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cancer research and clinical oncology 60 (1955), S. 291-293 
    ISSN: 1432-1335
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 18 (1966), S. 163-171 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Preparations of tick-borne encephalitis virus, which had been purified by protamine precipitation and inactivated at 37° C, were uniform on centrifugation in a sucrose density gradient. The inactivation destroys the infectivity but does not inhibit the haemagglutination reaction, the optimum of which is pH 6.4. A method is described, which allows the determination of the haemagglutinin titre after exposure of the preparations to different hydrogen ion concentrations. The preparations were only stable at pH values exceeding 7.3. At pH 7.5 the ability to haemagglutinate was lost if the preparations were heated at about 50° C. This ability is also destroyed by trypsin. It is therefore concluded that the haemagglutinin is a protein at the surface of the virus particle.
    Notes: Zusammenfassung Präparationen von FSME-Virus, die durch Protaminfällung gereinigt sind und bei 37° C inaktiviert wurden, erwiesen sich im Dichtegradienten als einheitlich. Das pH-Optimum der Reaktion war 6,4. Eine Methode wird beschrieben, die es ermöglicht, den HA-Titer derartiger Präparationen zu bestimmen, nachdem sie verschiedenen Wasserstoffionenkonzentrationen ausgesetzt waren. Die Präparationen waren nur stabil bei pH-Werten größer als 7,3. Bei pH 7,5 ging die Hämagglutinationsfähigkeit durch Erwärmung auf etwa 50° C verloren. Da die Hämagglutinationsfähigkeit auch durch Trypsin zerstört wurde, wird ein Eiweißmolekül als für die Hämagglutination verantwortlich angesehen.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Infection 16 (1988), S. 171-174 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Vor der geplanten Hepatitis-B-Impfung wurden 36 000 Angehörige von medizinischen Berufen auf HBs-Antigen, HBc-Antikörper und HBs-Antikörper getestet. Dabei wurde in 210 Sera HBs-Antigen und HBc-Antikörper nachgewiesen. 171 dieser Sera konnten auch mittels Hybridisierungstechnik auf virale Hepatitis B-DNS als Marker der Infektiosität untersucht werden. Nur bei 15 war virale Nukleinsäure nachweisbar. Bei 139 fanden sich HBe-Antikörper, von denen aber nur zwei HBV-DNS-positiv waren. Zwölf Personen, die alle HBV-DNS-negativ waren, hatten weder HBe-Antigen noch HBe-Antikörper. Hingegen wurde virale Nukleinsäure bei 13 von 20 Sera gefunden, die HBe-Antigen, aber keine HBe-Antikörper enthielten. Unsere Untersuchung bestätigt die epidemiologische Beobachtung, daß dem medizinischen Personal kaum eine Rolle als Infektionsquelle für die Patienten zukommt.
    Notes: Summary Prior to hepatitis B vaccination, 36,000 persons of the medical staff were tested for HBs antigen, HBc antibodies, and HBs antibodies. 210 sera were found positive for HBs antigen and HBc antibodies. Of these sera, 171 were available for testing for hepatitis B virus DNA as a marker of infectivity by spot hybridization. DNA was detected in only 15. One hundred and thirty-nine had HBe antibodies but no detectable HBe antigen, and only two of these were hepatitis B virus DNA positive. 12 had neither HBe antigen nor HBe antibodies and none had hepatitis B virus DNA. Hepatitis B virus DNA was, however, detected in 13 of 20 HBe antigen-positive but HBe antibody-negative sera. Our study confirms epidemiological observations that medical staff hardly plays any role as a source of HBV infection for patients.
    Type of Medium: Electronic Resource
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