Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Heat-inducible expression system for a foreign gene in cultured tobacco cells using the HSP18.2 promoter of Arabidopsis thaliana (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 466-472 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5′-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream of the β-glucuronidase reporter gene (GUS). The resulting HSP18.2-GUS construct was introduced into BY2 cells by electroporation or Agrobacterium-mediated transformation. Transient expression of the HSP18.2 promoter in protoplasts was very low regardless of the heat shock. Although expression of the HSP18.2-GUS chimeric gene in the stable transformants of BY2 was hardly detected in culture at 25°C, the expression increased rapidly on the transcriptional level when the incubation temperature was shifted to 35–37°C. The optimal temperature for heat-shock induction was 37°C. After a 2-h incubation at 37°C, GUS activity was about 1000-fold greater than that before heat shock. The amount of GUS mRNA was maximum 2 h after heat shock, and then decreased gradually.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169945
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Heat-inducible expression system for a foreign gene in cultured tobacco cells using the HSP18.2 promoter of Arabidopsis thaliana (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 466-472 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5′-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream of the β-glucuronidase reporter gene (GUS). The resulting HSP18.2-GUS construct was introduced into BY2 cells by electroporation or Agrobacterium-mediated transformation. Transient expression of the HSP18.2 promoter in protoplasts was very low regardless of the heat shock. Although expression of the HSP18.2-GUS chimeric gene in the stable transformants of BY2 was hardly detected in culture at 25°C, the expression increased rapidly on the transcriptional level when the incubation temperature was shifted to 35–37°C. The optimal temperature for heat-shock induction was 37°C. After a 2-h incubation at 37°C, GUS activity was about 1000-fold greater than that before heat shock. The amount of GUS mRNA was maximum 2 h after heat shock, and then decreased gradually.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050583
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    X-rays from the eclipsing pulsar 1957 +20 (1992)
    [s.l.] : Nature Publishing Group
    Nature 359 (1992), S. 303-304 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The primary star of the PSR1957 + 20 system is a pulsar of period 1.6ms, which is eclipsed in the metre waveband for -50 minutes out of each 9.16-hour binary orbit. Timing the pulsar allows the mass of the companion to be measured at -0.025 solar masses (Má©), well below the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/359303a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    QTL analysis of yield and seed number in Citrus (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 487-493 
    Details
    ISSN: 1432-2242
    Keywords: Key words Fruit breeding ; GxE interaction ; Yield components ; Seedless fruits ; Parthenocarpy ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Amount, regularity and low seed content of the crop are important properties of scion citrus cultivars. The genetic control of these traits was studied in a progeny derived from the cross Citrus volkameriana×Poncirus trifoliata using molecular marker analysis. Since the traits were not normally distributed, the Kruskal-Wallis non-parametric test was used for quantitative trait loci (QTLs) detection. Most of the QTLs detected correspond to the trait ”number of fruits per tree”, in agreement with its known physiological complexity. Related traits (fruit number, fruit size and seed number) are controlled by QTLs some of which are located in the same genomic regions, suggesting that undesired associations could be broken to some degree by recombination. QTL analysis over years revealed important effects of genotype-by-environment interaction on QTL detection. This result agrees with the differences found for the trait means among years, which was found to be related, among other causes, to the alternate bearing of some genotypes and the amount of rain before harvest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051507
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of some compounds isolated fromCelaenodendron mexicanum standl (euphorbiaceae) on seeds and phytopathogenic fungi (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 1025-1037 
    Details
    ISSN: 1573-1561
    Keywords: Biological effects ; Celaenodendron mexicanum ; Euphorbiaceae ; friedelin ; maytensifolin B ; ginkgetin ; bilobetin ; amentoflavone ; 3β-hydroxyfriedelan-16-one ; Amaranthus leucocarpus ; Echinochloa crusgalli ; Alternaria sp. ; Fusarium sp. ; Helminthosporium sp. ; phytopathogenic fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The known compounds friedelin, maytensifolin B, ginkgetin, bilobetin, and amentoflavone as well as the new triterpene 3β-hydroxyfriedelan-16-one were isolated fromCelaenodendron mexicanum (Euphorbiaceae), an endemic tree of the Pacific Ocean coast of Mexico. The compounds' structures were established on the basis of spectral analysis. The biological effects of aqueous leachates, a CHCl3-MeOH extract and the isolated compounds of leaves and twigs were evaluated on the radicle growth of two plants,Amaranthus leucocarpus andEchinochloa crusgalli, and on the radial growth of three phytopathogenic fungi,Fusarium sp.,Helminthosporium sp., andAlternaria sp. The organic extracts of both leaves and twigs inhibitedAmaranthus and stimulatedEchinochloa radicle growth. On the contrary, friedelin and maytensifolin B stimulatedAmaranthus and inhibitedEchinochloa. The target fungi showed a different response to each treatment, from inhibition to stimulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00980060
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...