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  • 1
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    Rudhard Stollberg: Arbeitssoziologie (Book Review) (1979)
    Berlin : Periodicals Archive Online (PAO)
    Deutsche Zeitschrift für Philosophie. 27:4 (1979) 510 
    Details
    ISSN: 0012-1045
    Topics: Philosophy
    Notes: REZENSIONEN
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:4085-1979-027-04-000015
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  • 2
    Electronic Resource
    Electronic Resource
    Granulocyte-macrophage colony-stimulating factor binding sites and oxidative metabolism in human granulocytes (1989)
    Springer
    Annals of hematology 59 (1989), S. 486-492 
    Details
    ISSN: 1432-0584
    Keywords: GM-CSF Receptor ; Granulocytes ; Oxidative metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated the interaction between GM-CSF and its receptor on human granulocytes and on several human tumor cell lines. Specific high-affinity binding for GM-CSF was characterized by Scatchard plot analysis. The specific radioactivity of the 125I-labeled derivative of rH. GM-CSF was determined by self-displacement analysis and calculated to be 30 μCi/μg. The maximum concentration of binding sites (B max) in granulocytes was 40 fmol/mg protein (2,200 molecules GM-CSF bound/cell) and the dissociation constant (KD) was 0.42 nM. No binding sites for GM-CSF were found in two lung cancer cell lines, SCLC-16HV and NCI-N417 or in the urinary bladder carcinoma cell line 5637, whereas the promyelocytic leukemia cell line HL60 was positive for GM-CSF binding. Time course experiments showed maximum binding of GM-CSF in granulocytes after an incubation period of 60 min and a decrease in binding after an incubation period of 2 h. In parallel, we found a maximum biological signal when granulocytes were preincubated for 90 min with GM-CSF, and a decrease after an incubation time of 120 min. Preincubation of the cells with rH. GM-CSF induced an enhancement of the production of activated oxygen species by the cells in response to PMA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329493
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  • 3
    Electronic Resource
    Electronic Resource
    Epidermal growth factor receptor expression, proliferation, and colony stimulating activity production in the urinary bladder carcinoma cell line 5637 (1987)
    Springer
    Journal of cancer research and clinical oncology 113 (1987), S. 579-585 
    Details
    ISSN: 1432-1335
    Keywords: Cell line 5637 ; EGF ; Receptor ; Growth-CSA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Addition of epidermal growth factor (EGF) to cultures of the urinary bladder carcinoma cell line 5637 regulated proliferation and production of colony stimulating activity (CSA). The optimal concentration range of EGF for stimulation of cell proliferation was 5–20 ng/ml EGF and for production of CSA 2–20 ng/ml EGF. High EGF concentrations (100–200 ng/ml) showed inhibitory effects on proliferation and to a greater extent on CSA production. Also, EGF binding sites of high affinity (kD: 3.25 nM) were demonstrated on the cell surface. In the optimal concentration range for stimulation (5–20 ng/ml EGF) EGF binding sites were occupied half-maximally. The loss in EGF binding after long incubation at 37°C was prevented by the lysosomal inhibitory agent, chloroquine. Nonspecific binding of EGF was very low, the amount of maximally bound EGF was 1430 fmol/mg protein (130,000 bound EGF molecules/cell). A strong band of approximately 170,000 daltons could be detected by means of an anti-erbB serum which recognizes the EGF receptor protein. The protein became phosphorylated upon addition of γ-32P ATP. The data suggest that EGF initiates its action by binding to specific high affinity receptors and plays a role in growth regulation and differentiation of the urinary bladder carcinoma cell line 5637.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390870
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