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  • 1
    Electronic Resource
    Electronic Resource
    A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells (1986)
    Springer
    Cancer immunology immunotherapy 23 (1986), S. 185-191 
    Details
    ISSN: 1432-0851
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The murine monoclonal antibody (MAb) BW 494 was characterized in relation to its tissue specificity, the epitope recognized, in vitro and in vivo radiolocalization and its potential to mediate antibody dependent cellular cytotoxicity (ADCC) and complement mediated cytolysis (CMC). The MAb defined carbohydrate epitope located on a 〉200 k daltons glycoprotein was mainly expressed on the majority of well differentiated adenocarcinomas of the pancreas. Furthermore, the epitope is accessible to MAb BW 494 in vivo, allowing an enrichment of radioactive antibody at the tumor site in nude mice. Additionally, MAb BW 494 is able to use human peripheral blood lymphocytes as effector cells for ADCC reactions against appropriate tumor target cells in vitro. In contrast, the antibody does not mediate human or rabbit CMC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205648
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative considerations supporting the irrelevance of circulating serum CEA for the immunoscintigraphic visualization of CEA expressing carcinomas (1988)
    Springer
    European journal of nuclear medicine 14 (1988), S. 523-528 
    Details
    ISSN: 1619-7089
    Keywords: CEA ; Conformational change ; MAb ; Immunoscintigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Starting from the phenomenon that the amount of circulating CEA in patients' sera did not significantly influence immunoscintigraphic visualization of CEA expressing tumors, we built up an in vitro model to explain this phenomenon. Blocking experiments in this model system showed that the CEA specific MAbs BW 431/26 and BW 431/31 could not be inhibited in their binding to cell associated CEA, if they were preincubated with a 20 molar excess of serum CEA. In contrast, the CEA-NCA cross reactive MAbs could be inhibited in their binding to tumor associated CEA under identical conditions. These data combined with western blotting analysis of patients' sera and affinity constant determinations argue that conformational changes in serum CEA cause a decreased affinity of the CEA specific MAbs to serum CEA allowing a preferential binding to tumor associated CEA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286769
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  • 3
    Electronic Resource
    Electronic Resource
    Neue Anwendungen der Dünnschichtchromatographie (1962)
    Springer
    Cellular and molecular life sciences 18 (1962), S. 430-432 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02151505
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  • 4
    Electronic Resource
    Electronic Resource
    Concentration of placental protein 10 (PP10) in maternal serum and amniotic fluid throughout normal gestation and in pregnancy complicated by fetal growth retardation (1983)
    Springer
    Archives of gynecology and obstetrics 233 (1983), S. 165-174 
    Details
    ISSN: 1432-0711
    Keywords: Placental Protein 10 (PP10) ; Radioimmunoassay ; Normal pregnancy ; Fetal growth retardation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary PP10, a new placental glycoprotein, was studied by a specific and sensitive double-antibody radioimmunoassay in maternal serum and other body fluids throughout pregnancy. The mean value of serum PP10 in healthy nonpregnant individuals was approximately 10 μU/l. During normal pregnancy it rose to 3,500 μU/l. The rate of rise was obtained from 78 normal pregnancies with 279 single assay values from weeks 6–40. The shape of the curve resembled that for other placental proteins (HPL, SP1). PP10 levels in amniotic fluid were measured in 145 samples from weeks 13–55 of normal pregnancies and at term. The mean concentration was 500 μU/l until week 18 and then rose slowly. Cord blood contained only trace amounts. PP10 was not found in maternal urine. The concentration in maternal serum and amniotic fluid was higher in twin pregnancies than in singleton pregnancies. In 46 cases with low birth weights the PP10 levels in maternal serum were significantly lower than normal. Simultaneous measurements of PP10 and E3, HPL and SP1 were made in 17 individual follow-up's. PP10 was comparable with E3 and appeared to be better than HPL and SP1 in predicting intrauterine fetal growth retardation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02114597
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  • 5
    Electronic Resource
    Electronic Resource
    Serum levels of placental protein 10 (PP10) in women with breast cancer and genital carcinoma and in healthy male and female subjects (1983)
    Springer
    Archives of gynecology and obstetrics 233 (1983), S. 267-274 
    Details
    ISSN: 1432-0711
    Keywords: Placental Protein 10 ; PP10 ; Radioimmunoassay ; Breast Cancer ; Genital Cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary PP10, a recently characterized glycoprotein from human placenta, was studied using a specific double-antibody radioimmunoassay in the serum of about 100 volunteers and 200 cancer patients. Elevated levels (〉 20 nU/ml) were found in 87% of patients with primary breast cancer, in 100% of those with primary genital tumours and in 78% of patients with recurrent disease. PP10 was also measured in tumour extracts and in some patients with benign tumours. The serum concentration decreased within a few weeks after removal of the tumour. There were no significant correlations of the PP10 level with age, tumour size, histological grading or lymph node involvement. Sequential determinations of PP10 during cytostatic therapy sometimes showed rising levels accompany the development of metastases. PP10 can be regarded as a tumour associated protein and a tumour marker in gynaecological practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133801
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  • 6
    Electronic Resource
    Electronic Resource
    Adsorptionschromatographie an vernetztem Polymethacrylat (1964)
    Springer
    Naturwissenschaften 51 (1964), S. 138-138 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00631154
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  • 7
    Electronic Resource
    Electronic Resource
    Granulocyte-macrophage colony-stimulating factor binding sites and oxidative metabolism in human granulocytes (1989)
    Springer
    Annals of hematology 59 (1989), S. 486-492 
    Details
    ISSN: 1432-0584
    Keywords: GM-CSF Receptor ; Granulocytes ; Oxidative metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated the interaction between GM-CSF and its receptor on human granulocytes and on several human tumor cell lines. Specific high-affinity binding for GM-CSF was characterized by Scatchard plot analysis. The specific radioactivity of the 125I-labeled derivative of rH. GM-CSF was determined by self-displacement analysis and calculated to be 30 μCi/μg. The maximum concentration of binding sites (B max) in granulocytes was 40 fmol/mg protein (2,200 molecules GM-CSF bound/cell) and the dissociation constant (KD) was 0.42 nM. No binding sites for GM-CSF were found in two lung cancer cell lines, SCLC-16HV and NCI-N417 or in the urinary bladder carcinoma cell line 5637, whereas the promyelocytic leukemia cell line HL60 was positive for GM-CSF binding. Time course experiments showed maximum binding of GM-CSF in granulocytes after an incubation period of 60 min and a decrease in binding after an incubation period of 2 h. In parallel, we found a maximum biological signal when granulocytes were preincubated for 90 min with GM-CSF, and a decrease after an incubation time of 120 min. Preincubation of the cells with rH. GM-CSF induced an enhancement of the production of activated oxygen species by the cells in response to PMA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329493
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  • 8
    Electronic Resource
    Electronic Resource
    Beitrag zur radioimmunologischen Bestimmung von humanem Placentalactogen (1970)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 252 (1970), S. 271-274 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Eine radioimmunologische Methode zur Bestimmung von humanem Placentalactogen (HPL) wird beschrieben. Neben der Bewertung der Antiseren und einer Prüfung verschiedener Trennmethoden werden Möglichkeiten zur Prüfung des radiojod-markierten Hormons diskutiert und eine Anzahl von Seren analysiert. Die HPL-Werte, die gegen ein kristallisiertes HPL-Präparat als Standard erhalten wurden, streuen von 2,7–6,2 μg/ml. Die Methode der adsorptiven Trennung des freien, ungebundenen Hormons aus dem Inkubationsgemisch mit Amberlite CG-400 I stellte sich bei dieser Untersuchung als besonders geeignet heraus.
    Notes: Abstract The estimation of the antiserum, the examination of different methods for separation of bound and unbound activity and some tests for the iodinated hormon are discussed. Finally several sera are analysed, taken from women during last month of pregnancy. Against crystallized HPL as laboratory standard in some sera the content varies from 2.7 to 6.2 μg/ml. The separation of the free, unbound hormone from the incubation mixture with Amberlite CG 400 I proved to be the best qualified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571349
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  • 9
    Electronic Resource
    Electronic Resource
    Antamanid. Seine Entdeckung, Isolierung, Strukturaufklärung und Synthese (1968)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 80 (1968), S. 209-213 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Als Antamanid bezeichnen wir einen Inhaltsstoff des grünen Knollenblätterpilzes, Amanita phalloides, der an der weißen Maus die tödliche Wirkung der Amanitatoxine Phalloidin und α-Amanitin bei spätestens gleichzeitiger Verabreichung aufhebt. Die Konzentration im Pilz ist allerdings so gering, daß dessen Giftwirkung überwiegt. Antamanid ist ein cyclisches Dekapeptid aus den L-Aminosäuren Alanin, Phenylalanin, Prolin und Valin, die zueinander im Molverhältnis von 1:4:4:1 stehen. Die Sequenz wurde durch Gaschromatographie-Massenspektrometrie aufgeklärt. Die Synthese auf einem klassischen Weg der Peptidchemie bewies die Richtigkeit der Strukturformel.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19680800602
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  • 10
    Electronic Resource
    Electronic Resource
    The Discovery, Isolation, Elucidation of Structure, and Synthesis of AntamanidePart 36 of the Chemical Composition of Amanita phalloides. Part 36: Th. Wieland and R. Jeck, Liebigs Ann. Chem., in press. (1968)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 7 (1968), S. 204-208 
    Details
    ISSN: 0570-0833
    Keywords: Antamanide ; Natural products ; Peptides ; Amanita phalloides ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Antamanide is the name we have given to a constituent of the fungus Amanita phalloides. This substance counteracts the lethal action of the Amanita toxins phalloidine and α-amanatine if administered to the white mouse before, or simultaneously with, the poisons. Its concentration in the fungus is, however, so low that the toxic action of the latter predominates. Antamanide is a cyclic decapeptide formed from the L-amino acids alanine, phenylalanine, proline, and valine in the molar ratio 1:4:4:1. The amino-acid sequence was determined by a combination of gas chromatography and mass spectrometry. The structural formula assigned was confirmed by synthesis according to a classic route of peptide chemistry.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.196802041
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