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  • 1
    Electronic Resource
    Electronic Resource
    High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus (1994)
    Springer
    Archives of virology 136 (1994), S. 375-380 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large quantity. Electron microscopic examination of these cells showed the presence of numerous potyvirus-like particles in the cytoplasm. Morphologically the particles resembled potyvirus particles assembled in vitro in the absence of viral RNA and those found inEscherichia coli expressing the recombinant CP gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01321065
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of Alumina Scales Formed During Isothermal and Cyclic Oxidation of Plasma-Sprayed TBC Systems at 1150°C (1999)
    Springer
    Oxidation of metals 52 (1999), S. 31-76 
    Details
    ISSN: 1573-4889
    Keywords: SCALE FRACTURE ; OXIDE SCALES ; ALUMINA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The isothermal- and cyclic-oxidation behavior ofthermal barrier coating (TBC) systems consisting ofvacuum plasma-sprayed (VPS) Ni-22Cr-10Al-1Y (wt.%) bondcoatings and air plasma-sprayed (APS)Y2O3-stabilized ZrO2 (YSZ) top coatings (onsingle-crystal superalloys) was investigated. Themicrostructures, flaw contents, and fracture behavior ofthe Al2O3 scales formed duringoxidation testing at 1150°C were characterized (by analysis of coating andscale fracture surfaces and metallographic crosssections). Significant localized fracture and bucklingof the Al2O3 scales that formedalong the bond-coat-top-coat interfaces were observed after cyclic oxidationof TBCs. However, substantial amounts of localized scaledamage did not induce rapid TBC failure. Decohesion ofthe columnar alumina scales on the rough bond-coat surfaces occurred by both internalAl2O3 fracture (parallel to themetal surface) and oxide-metal delamination. There weremicrostructural indications ofAl2O3 scale crack healing bysintering into planar arrays of voids. Alumina scales that formed onconvex NiCrAlY surfaces (with radii of 50 μm or less)often contained significant amounts of internal voids(along grain boundaries) after cyclic oxidation, whereas scales formed by isothermal oxidationcontained few visible voids. Accelerated void growth inAl2O3 scales on the irregularNiCrAlY surfaces appeared to be creep-related and wasattributed to the synergistic effects of geometric and thermalstresses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018870923397
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Application of infrared imaging to the study of controlled failure of thermal barrier coatings (2000)
    Springer
    Journal of materials science 35 (2000), S. 2643-2651 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A technique that uses high resolution infrared (IR) imaging was developed to track and analyze damage evolution of thermal barrier coatings (TBCs) during controlled mechanical testing of a TBC specimen. Coating debonding and spallation were examined during a monotonic load-to-TBC-failure test. The infrared imaging, in concert with a controlled thermal gradient in the specimen, was particularly effective in identifying and tracking localized damage evolution because the damage in the TBC was always associated with a measurable surface-temperature change. It is demonstrated that the combined use of high-resolution infrared imaging and controlled mechanical testing of TBCs is an effective method to characterize the evolution of their failure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004781220825
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    Paper (German National Licenses)
    Fulltext
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