ISSN:
1433-2981
Schlagwort(e):
NMR
;
Rabbit
;
Rat
;
Red blood cells
;
Sheep
;
Water permeability
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The diffusional water permeability (P d) of the red blood cell (RBC) membrane of rat, rabbit and sheep, representing some animal species introduced to Australia, has been monitored, by a Mn2+-doping 1H nuclear magnetic resonance (NR) technique using a high-field spectometer operating at 400 MHz. In order to make comparisons with previous measurements on the same species (performed at 25 MHz) an analysis of the influence of instrumental parameters and of the state of blood oxygenation was performed on samples of rat and rabbit blood. It was found that by using a short interpulse delay (around 100 µs) in the Carr-Purcell-Meiboom-Gill sequence, and by performing the measurements of the transverse relaxation time of the water in the cell interior (T 2i) on packed cells (haematocrit 〉95%), the values of the water exchange time (T e) obtained with a high-field spectrometer are comparable with those obtained by using a low-field NMR spectrometer. The values of the diffusional water permeability (P d), calculated from the T e values, were, at 24.6°C around 10 × 10−3 cm/s in case of rat and rabbit RBC and around 5 × 10−3 cm/s for sheep RBC; at 37°C the P d values were around 16 × 10−3 cm/s for rat RBC, 14 × 10−3 cm/s for rabbit RBC and 7 × 10−3 cm/s for sheep RBC. These values are very close to the P d values previously reported for the European counterparts of these species. There were no significant differences in the P d values between laboratory rabbits and wild rabbits, or between castrated males, pregnant or nonpregnant females. These results suggest that no changes in the RBC water permeability are correlated with marked alterations in the habitat of animal species introduced to Australia or by sex hormone status. If the NMR instrument-parameters are carefully chosen then comparative studies of RBC water permeability in different laboratories, at separate locations and using different instruments are meaningful.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00185179
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