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  • 1
    Electronic Resource
    Electronic Resource
    “Arabidobrassica”: A novel plant obtained by protoplast fusion (1980)
    Springer
    Planta 149 (1980), S. 112-117 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis ; Brassica ; Chromosomal elimination ; Hybridization, somatic ; Somatic hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. Shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. Most regenerants show morphological abnormalities and unusual organizational patterns. Flowering forms have so far been sterile. “Asymmetric” hybrids (i.e., hybrids bearing most genetic material of one of the parent species and only few chromosomes of the other) were more regular in morphology. The results represent the first case of intergeneric-intertribal hybridization of flowering plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380870
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic antibiotic resistance may be differentially silenced in germinating pollen grains (1988)
    Springer
    Plant cell reports 7 (1988), S. 542-545 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic tobacco (Nicotiana tabacum L.) plants, carrying the neomycin phosphotransferase (NPT II) gene from E. coli, are resistant to kanamycin when grown from seeds on kanamycin containing medium. Tissue and cell cultures derived from those transformants also express resistance and regenerate complete plantlets in the presence of the antibiotic. This unspecific response to the selective condition has led to the belief that the foreign gene is continuously active or uniformly inducible in all cells of the transgenic plant. However, our experiments show that this view is not true for pollen grains during in vitro germination. Pollen grains isolated from kanamycin resistant tobacco plants carry and transmit the foreign gene but do not express resistance when germinating in vitro. This data presents evidence for differential silencing of a foreign gene in a mature gamete. On the other hand, immature pollen grains (microspores) appear to express resistance. The point of the downregulation of the neomycin transferase gene during pollen maturation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272752
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    “Arabidobrassica”: Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells (1981)
    Springer
    Planta 153 (1981), S. 586-593 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis ; Brassica ; Chromosome elimination ; Protoplast fusion ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A somatic hybrid cell line, cloned from an individual protoplast-fusion product between Arabidopsis thaliana and Brassica campestris, gave rise to formation of numerous plants differing drastically in morphology. Analysis of these various regenerants, all of which originated from one and the same heterokaryon derived from the fusion of two cells, shows the unspecific elimination of chromosomes of both parental species during the callus growth phase. Whereas the parental cells have so far not been sucessfully regenerated into plants, several of their different asymmetric hybrids are capable of morphogenesis. Furthermore, chromosomal analysis indicates extensive recombination. Most of the plants are predoinantly morphologically regular. Abnormalities are mostly limited to the flowers which tend to undergo phyllody. The results demonstrate that remote somatic hybridization may have applications although true amphidiploids may not be obtainable. The transfer of small units of genetic material between distantly related species by protoplast fusion seems to be a more realistic approach than the combination of complete, highly diverse genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385546
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  • 4
    Electronic Resource
    Electronic Resource
    “Arabidobrassica”: Plant-genome engineering by protoplast fusion (1979)
    Springer
    Naturwissenschaften 66 (1979), S. 547-554 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Abstract After fusion of somatic cells (isolated protoplasts) of Arabidopsis thaliana and Brassica campestris, plants could be regenerated in which genetic material from parents of taxonomically different tribes is combined. Between these regenerants asymmetric hybrids have also been obtained, in which one parental genome is represented by reduced chromosome numbers. Results concerning the analysis and genesis of these plants are used to discuss the question whether such monster plants are of any importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368808
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  • 5
    Electronic Resource
    Electronic Resource
    Pflanzliche Zellkulturtechniken als Züchtungsschritt am Beispiel Raps (1980)
    Springer
    Naturwissenschaften 67 (1980), S. 301-306 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Abstract A supplementation of classical plant breeding is now necessary due to the limitations imposed by available genetic variability and the slowness of the method. Therefore, cell culture techniques could play an important role in the future. Using rape seed, in which plants derived from anther culture and in vitro mutagenesis are already field tested, it has been shown that, in this case, somatic genetics is very close to becoming a practical method. For most of the other crop plants, in particular the cereals, no such unconventional breeding techniques have yet been satisfactorily established for commercial use.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01153500
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  • 6
    Electronic Resource
    Electronic Resource
    The separation of viable rye microspores from mixed populations and their development in culture (1975)
    Springer
    Molecular genetics and genomics 138 (1975), S. 293-297 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A technique is described to isolate rye microspores from anthers precultured on agar and from intact spikes. Highly purified fractions consisting predominantly of viable late uninucleate or early binucleate microspores are obtained by filtration and centrifugation techniques. The preparations form an excellent starting material to attempt to increase the frequency of microspore plantlet formation above that normally obtained by anther culture. Microspores isolated from anthers precultured for five or six days on agar, develop after three weeks culture in a liquid medium into multicellular structures, the largest of which burst through the microspore wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264798
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Protoplast regeneration and stem embryogenesis of haploid androgenetic rape (1976)
    Springer
    Molecular genetics and genomics 145 (1976), S. 245-247 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Haploid microspore-derived plants from amphidiploid Brassica napus give rise in culture to very large numbers of secondary stem embryoids from single epidermal cells. The embryoids can be excised, transferred to fresh medium and induced to repeat the process 4–6 times. Eventually the embryoids form larger plantlets which can be grown to maturity. Spontaneous amphihaploid plants or microspore-derived plants growing either in vitro or in vivo have been used as a source of plant protoplasts. Such protoplasts have been induced to produce calluses capable of both root and shoot production. The limited number of plantlets so far obtained from large numbers of calluses have retained their haploidy. The significance of haploid single-cell cultures of such agriculturally important fodder crops is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00325819
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  • 8
    Electronic Resource
    Electronic Resource
    Hybrid cell lines Arabidopsis thaliana + Brassica campestris: No evidence for specific chromosome elimination (1978)
    Springer
    Molecular genetics and genomics 165 (1978), S. 257-264 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts from callus tissue of Arabidopsis thaliana and from leaves of Brassica campestris were fused using the PEG-high pH-high Ca++-technique. Single dividing fusion products were isolated mechanically and cultured further in 1 μl microdroplets. 31 cell lines each originating from a single fusion event have been obtained. Cytological analysis of 6 cell lines was conducted 4–7 months after isolation. All metaphases examined contained chromosomes of both species. Both Arabidopsis and Brassica specific chromosomes were still retained in cells of 7 month old cultures. Reconstituted di- and multiconstrictional chromosomes were also observed. Biochemical analysis of 12 cell lines was performed in 6–7-month old cultures. Electrophoresis on PAG and specific staining for esterase, lactate dehydrogenase, and peroxidase activities revealed isozymes of both parents to be present in all cell lines. The data obtained are interpreted as evidence for retention and functional activity in hybrid cells of specific chromosomes from each species for at least 7 months of culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332524
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  • 9
    Electronic Resource
    Electronic Resource
    „Senföle“ (1978)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 12 (1978), S. 182-188 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19780120603
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Phytohormone - Werkzeuge pflanzlicher Zellkulturtechniken (1977)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 11 (1977), S. 108-117 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19770110403
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    Paper (German National Licenses)
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