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  • 1
    ISSN: 0167-0115
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Regulatory Peptides 40 (1992), S. 172 
    ISSN: 0167-0115
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Regulatory Peptides 40 (1992), S. 255 
    ISSN: 0167-0115
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Acta neuropathologica 69 (1986), S. 267-271 
    ISSN: 1432-0533
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In gerbils the hemispheric blood flow was interrupted for 5 min by bilateral carotid artery occlusion to produce delayed selective destruction of the CA 1 sector of hippocampus. The influence of hemodynamic factors was studied by evaluating the microcirculation before and at two times after ischemia (3 min and 7 days), using Evans blue as an intravital vascular tracer. The density of perfused capillaries and the fractional volume of circulating blood were determined by quantitative morphometry and the values for the vulnerable CA 1 sector compared with those for the resistent CA 3 sector and cerebral cortex. In control animals the number of perfused capillaries in the CA 1 sector was about 20% lower, and the volume of circulating blood about 30% lower, than in the CA 3 sector or cerebral cortex. This difference was markedly enhanced after 5-min ischemia. During the early recirculation phase, capillary perfusion improved in the cortex, whereas in the CA 1 sector (and to a lesser degree also in the CA 3 sector) it declined. After 7 days, the density of perfused capillaries and the volume of circulating blood had returned to control levels in the cerebral cortex and CA 3 sector of hippocampus. In the CA 1 sector, in contrast, the microcirculation had further deteriorated. The density of perfused capillaries was less than 30%, and the circulating blood volume even less than 50%, of that in the cerebral cortex. The results obtained indicate that the microcirculatory capacity of the selectively vulnerable CA 1 sector of hippocampus is distinctly lower than that of resistant areas of the brain and suggest that hemodynamic factors may contribute to the difference in vulnerability of these regions to short-lasting cerebral ischemia.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Journal of cancer research and clinical oncology 115 (1989), S. 388-392 
    ISSN: 1432-1335
    Schlagwort(e): Gastrin ; Nude mice ; Cell culture ; Colorectal carcinoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The growth-regulating effects of pentagastrin, gastrin and the gastrin-receptor antagonist proglumide were investigated in three established cell lines derived from human colorectal carcinomas in vitro and after transplantation into nude mice. In vitro a significant increase of cell growth in the SW 403 cell line incubated with pentagastrin or gastrin was observed. In the Lovo cell line this effect was only detected after synchronization of cell growth. Pentagastrin and gastrin had no effect on the growth of the Ls 174 T cell line. Proglumide reduced cell proliferation in all three cell lines as well as in the L929S cell line derived from fibroblasts, which served as control. After transplantation into nude mice all tumor cell lines increased, Love and Ls 174 T as undifferentiated tumor, SW 403 as differentiated. Pentagastrin increased and proglumide decreased growth in SW 403 tumors, whereas no effect was observed on Ls 174 T and Lovo tumors. We therefore conclude that growth of some colorectal carcinomas is regulated by gastrin, but that the effect of proglumide is unspecific rather than related to blockage of gastrin receptors. The growth-regulating effect of gastrin could be due to tumor differentiation.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Journal of cancer research and clinical oncology 121 (1995), S. 661-666 
    ISSN: 1432-1335
    Schlagwort(e): Colorectal carcinoma ; Gastrin ; Gastrin-binding protein ; Gastrin/CCK-B receptor ; RNase protection assay
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract To investigate further the presence of an autocrine proliferative loop involving gastrin in colorectal carcinomas and to clarify the receptor responsible, 102 human colorectal carcinomas and 10 hepatic metastases were investigated for the expression of the genes encoding gastrin, the gastrin/CCK-B receptor and the gastrin/CCK-C receptor. Levels of RNA expression were assayed by RNase protection assay. In addition, gastrin/CCK receptors on crude membranes of tumour tissue were assayed by radioligand binding. High-affinity gastrin/CCK-B receptors were not detected in any of the carcinomas investigated, whereas in 36% low-affinity binding was observed, consistent with the expression of the gastrin/CCK-C receptor. RNase protection assay detected the RNA for the gastrin/CCK-B receptor in 11% of the carcinomas investigated, whereas the RNA for the gastrin/CCK-C receptor was demonstrated in 75% and the RNA for gastrin in 86% of the carcinomas investigated. These results confirm the recent demonstration of progastrin fragments in colorectal carcinomas. One possible explanation for progastrin expression is that such progastrin fragments may participate in an autocrine proliferative loop. The receptor involved in this loop is more likely to be the low-affinity gastrin/CCK-C receptor rather than the gastrin/CCK-B receptor, which is rarely expressed in colorectal carcinomas.
    Materialart: Digitale Medien
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  • 7
    ISSN: 1432-1335
    Schlagwort(e): Ornithine decarboxylase ; 2-difluoromethyl-ornithine ; Colonic carcinoma cell lines ; Gastrin ; Gastrin receptor blockers ; EGF
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Polyamines are essential factors of cell growth and differentiation. Modulation of the cellular polyamine content by 2-difluoromethylornithine (DFMO) inhibiting ornithine decarboxylase (ODC), or by hormones inducing ODC, influences cell growth. Gastrin acts trophically on some colonic carcinomas and their growth is inhibited by gastrin receptor blockers. The mechanism of the trophic action of gastrin on colonic carcinomas is not known. In this study the effect of gastrin, gastrin receptor blockers, epidermal growth factor (EGF) and DFMO on growth and ODC activity of four human colon carcinoma cell lines (SW 403, SW 1116, LS 174 T and Lovo) was investigated. Growth and ODC activity of all cell lines were inhibited by DFMO. Growth of the SW 403 cell line was increased by gastrin and inhibited by the gastrin receptor blocker benzotrypte. The other cell lines did not respond to gastrin and the gastrin receptor blocker. In SW 403 cells ODC activity was increased by gastrin, and was also elevated after treatment with the gastrin receptor blocker. These in vitro results were confirmed by studies on tumours that developed from SW 403 cells in nude mice. Combination of benzotrypte and DFMO did not enhance the antiproliferative effect. EGF increased growth of SW 403 cells, but no induction of ODC activity was measured. LS 174 T cells were not stimulated by EGF. Medium replacement was the strongest stimulus of ODC activity in SW 403 cells already inducing ODC after 3 h. During cell culture ODC activity was high after seeding and decreased continuously with increasing cell density. These data suggest that gastrin induces ODC in gastrin-sensitive colonic carcinoma cells. DFMO appears to be a valuable antiproliferative agent in colonic carcinoma cells.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Langenbeck's archives of surgery 375 (1990), S. 326-329 
    ISSN: 1435-2451
    Schlagwort(e): Esophageal carcinoma ; Bronchoscopy ; Bronchial carcinoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Von 1980–1987 wurden 95 Patienten mit einem Ösophaguskarzinom bronchoskopiert. Bei 67 Patienten (70,5%) wurde ein regelrechter Befund erhoben. 24 Patienten (25,3%) zeigten in der Bronchoskopie indirekte Zeichen des Tumorwachstums and 4 Patienten (4,2%) eine direkte Tumorinfiltration. Die Korrelation der Tumorausdehnung mit dem bronchoskopischen Befund ergab eine deutliche Zunahme pathologischer bronchoskopischer Befunde bei einer Tumorlönge über 5 cm. Ösophaguskarzinome im proximalen Drittel waren etwa doppelt so hdufig mit pathologischen bronchoskopischen Befunden korreliert wie Karzinome im mittleren Drittel. Bei 5 Patienten (5,3%) sicherte die Bronchoskopie ein bis dahin unbekanntes zusdtzliches Bronchialkarzinom. Insgesamt wurden 67 der 95 Patienten einer operatven Therapie unterzogen. In 59 Fdllen erfolgte die Ösophagusresektion, bei 8 Patienten war lediglich eine Bypassoperation möglich. Die Operationsrate betrug im Kollektiv der bronchoskopischen Normalbefunde 77,6%. Dagegen waren nur die Hdlfte der Patienten mit einem pathologischen bronchoskopischen Befund operabel, die übrigen Patienten erhielten eine Strahlentherapie. Wir halten die Bronchoskopie wegen ihres Beitrages zur Kldrung der Operabilität bei Ösophaguskarzinomen and wegen der relativ hohen Rate von synchronen Neoplasien für eine sinnvolle Zusatzuntersuchung.
    Notizen: Summary Between 1980 and 1987 95 patients with esophageal carcinoma had a bronchoscopy. In 67 patients (70.5%) no pathological findings could be detected. In 24 patients (25.3%) signs of impingement were present and in 4 patients (4.2%) tumor infiltration into the tracheobronchial tree was visible. The correlation between tumor length and bronchoscopic findings revealed a marked increase of direct and indirect tumor evidence in esophageal carcinomas of more than 5 cm in length. Pathologic bronchoscopic findings were detected twice as often in patients with carcinoma of the upper third of the esophagus in comparison with those of the middle third. In 5 patients (5.3%) bronchoscopy revealed an unknown additional bronchial carcinoma. 67 of the 95 patients underwent operation. In 59 patients the esophagus was resected. Among the normal bronchoscopic findings the operability rate was 77.6%. In contrast, only half of the patients with pathological bronchoscopic findings were operable, the other patients received radiotherapy. We recommend, therefore, preoperative bronchoscopy as an important investigation for assessment of operability and for evaluation of synchronous neoplasms in patients with an esophageal carcinoma.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1435-2451
    Schlagwort(e): Key words Malignant soft tissue sarcoma ; FACScan ; DNA ploidy ; Cell cycle analysis ; Long-term survival
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Introduction: The prognostic significance of tumor DNA ploidy and cell cycle analysis for long-term survival has been examined in 19 patients with liposarcoma or malignant fibrous histiocytoma. In many cases, different tumor areas of primary tumors and local recurrences have been analyzed to reveal intratumoral heterogeneity. Results: Among the primary tumors, there were eight aneuploid tumors, three of which showed diploid and aneuploid tumor regions. Correlations among DNA ploidy, grading, percentage of S-phase cells and infiltrative growth pattern of the tumors could be demonstrated. Poorly differentiated tumors (G3) showed aneuploidy in six of eight patients. Aneuploid tumors showed S-phase cells in 17.2% (range 3.2–38.1%), which was higher than the percentage of S-phase cells in diploid tumors (9.4%, range 2.1–27.4%). Aneuploid tumors showed a more infiltrative growth pattern (6 of 8 patients) than diploid tumors (6 of 11 patients). The median survival time of patients with diploid tumors was 86.5 months (8–144 months), compared with 40.9 months (11–54 months) for patients with aneuploid tumors. Conclusion: DNA ploidy and percentage of S-phase cells may be considered as prognostic factors.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Langenbeck's archives of surgery 376 (1991), S. 152-157 
    ISSN: 1435-2451
    Schlagwort(e): Lower gastrointestinal hemorrhage ; Endoscopy ; Angiography ; Scintigraphy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung In einer retrospektiven Analyse wird die Wertigkeit der diagnostischen Verfahren bei der akuten unteren gastrointestinalen Blutung dargestellt. Die Blutungsquelle der 233 Patienten (1979–1988) lag distal des Treitz-Bandes; Hämorrhoidalblutungen wurden nicht aufgenommen. Nach Ausschluß der oberen gastrointestinalen Blutung erfolgte in der Regel als erster diagnostischer Schritt die Rekto-/Koloskopie, die in 77% die Blutungsursache bei den behandelten Patienten (n = 136) nachwies. Die Angiographie bei persistierender Blutung zeigte in 68% die Blutungsquelle; in Kombination mit der Rekto-/Koloskopie ließ sich in 86,5% der behandelten Patienten die Blutungsquelle lokalisieren. Die Erythrozytenszintigraphie steigerte den Anteil des positiven Lokalisationsnachweises dieser Patienten auf 89,7%. Die Sensitivitdt der Koloskopie war mit 0,93 deutlich höher als die der Angiographie mit 0,78 und der Erythrozytenszintigraphie mit 0,75. Unter den operationspflichtigen Blutungsursachen dominierten abgesehen von den Karzinomen and den Adenomen das Meckel-Divertikel und die Angiodysplasie. Endoskopische Therapieverfahren erfolgten bei 61 Patienten. 6,5% dieser Patienten mußten aber wegen persistierender Blutung später operiert wurden. Von den insgesamt 79 operierten Patienten wurden 31 ausschließlich wegen der Blutung operiert. 12 Patienten verstarben, 6 waren wegen der aktiven Blutung operiert wurden, 6 weitere aus onkologischen Gründen.
    Notizen: Summary In a retrospective analysis the diagnostic procedure was evaluated in patients with acute lower gastrointestinal bleeding. Bleeding sources were localized distally to the ligament of Treitz in 233 patients (1979–1988). Patients with hemorrhoidal bleeding were not included. Following exclusion of an upper gastrointestinal bleeding the diagnostic procedure was initiated with a recto-/colonoscopy. Lesions were detected in 77% of the treated patients (n=136). Angiography localized the bleeding in 68%, in combination with colonoscopy the identification of the bleeding source reached 86.5% in the treated patients. Following scintigraphy the bleeding source was determined in 89.7% of these patients. The sensitivity of colonoscopy (0.93) was superior to the angiography (0.78) and to the scintigraphy (0.75). Apart from neoplasms and adenomas angiodysplasia and Meckel's diverticula were the most common sources of the bleeding in patients who underwent operation. In 61 patients endoscopic therapy was performed, however, 6.5% of these patients had to be operated on later because of persistent bleeding. All together 79 patients underwent operation, 31 for bleeding and 48 for other reasons. 12 patients died, 6 of them were operated on for the bleeding, the other for neoplasms.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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