Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Patient-Specific Heterogeneity of Antinuclear Antibodies as Revealed by an Isoelectric Focusing Immunoblot System (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 26 (1987), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ninety-nine sera from patients with different rheumatic diseases (systemic lupus erythematosus, rheumatoid arthritis, progressive systemic sclerosis, and mixed and unidifferentiated connective tissue disease) were applied to a newly developed isoelectric focusing (IEF) immunoblot system for the demonstration of antinuclear antibodies. Nucleoproteins were separated according to their isoelectric points (pI) and immobilized onto nitrocellulose, and binding of serum antibodies was determined by an alkaline phosphatase labelled second antibody. 89.8% of all sera positive in indirect immunofluorescence assays with Hep 2 as substrate showed positive reactivity in IEF immunoblot. Furthermore, 88% of patients' sera negative on Hep 2 cells gave a positive reaction in IEF immunoblot. The predominant antibody banding pattern observed showed parallel bands in the acidic as well as the neutral pH ranges. Antibody specificities found in the IEF immunoblot system turned out to be patient-specific, but no marker antibody for a discrete disease entity was obtained. Even when monoclonal antibodies or WHO standard sera were applied to nuclear antigen they exhibited heterogeneity in their binding pattern. Bands with the same pI were observed using sera from patients with different rheumatic disease entities. Immunodeletion experiments suggest the recognition of identical antigenic proteins by the different patients' sera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02236.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a 58- and a 78-kD Monocytic Membrane Protein with Affinity to the Acetylcholine Receptor in Myasthenia Gravis Patients (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 39 (1994), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The autoimmune disease myasthenia gravis (MG), caused by the effect of specific antibodies, directed towards the nicotinic acetylcholine receptor, is triggered by autoantigen-specific T cells. In order to investigate cellular parts of the immune response in MG, the authors investigated the binding of the nicotinic acetylcholine receptor (AChR) to peripheral blood mononuclear cells (PBMC) from MG patients. AChR binding cells were identified by resetting experiments using AChR-coated fluoresceine beads. Applying this technique, a significant percentage of PBMC (21.2 × 7.65%) from MG patients formed rosettes with AChR-coated beads.Membrane preparations of nycodenz- or percoll-separated monocytes from MG patients or T-cell depleted monocytic subpopulations were applied to SDS-PAGE under reducing conditions. Ligand-blotting studies with biotinylated AChRs revealed two cell-membrane proteins with molecular weights of 58- and 78-kD. In parallel the same results were obtained by affinity chromatography of monocytic membrane proteins using AChR-sepharose. A possible interference of anti-AChR IgG was excluded. The 58- and the 78-kD proteins are detectable under reducing conditions by ligand blotting with AChR-biotin, while under non-reducing conditions only the 58-kD protein can be detected. Furthermore, in experiments using Endoglycosidase-H, the 58-kD protein appears to be non-glycosylated, while the 78-kD protein bears carbohydrates.These findings suggest that monocytes which bind the AChR via specific membrane proteins on their surface might act as antigen-presenting cells and may lead to an induction of the T-cell response, in the early phase of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1994.tb03376.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Expression cloning and partial sequence of a 235 KDA protein associated with the polar regions of mitotic spindles
    Amsterdam : Elsevier
    Cell Biology International Reports 14 (1990), S. 57 
    Details
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0309-1651(90)90334-U
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Isolation of human NuMA protein
    Amsterdam : Elsevier
    FEBS Letters 354 (1994), S. 307-310 
    Details
    ISSN: 0014-5793
    Keywords: Cell cycle regulation ; Centrosome ; Chromatin condensation ; RCC1 ; Ran ; RanBP2
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(94)01151-6
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    THYMIC MYOGENESIS, T-LYMPHOCYTES AND THE PATHOGENESIS OF MYASTHENIA GRAVIS (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 377 (1981), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1981.tb33753.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    QUANTITATIVE IONOPHORESIS AT MYASTHENIC RAT END PLATES (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 377 (1981), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1981.tb33819.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Heterogenität von Acetylcholinrezeptor-Antikörpern bei Myasthenia gravis Patienten (1984)
    Springer
    Journal of molecular medicine 62 (1984), S. 377-385 
    Details
    ISSN: 1432-1440
    Keywords: Myasthenia gravis ; Acetylcholine receptor antibody ; Antibody heterogeneity ; Cross-reactivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Antibodies against the nicotinic acetylcholine receptor in sera of 21 myasthenia gravis patients were checked for their ability to block or split binding of α-bungarotoxin to the human acetylcholine receptor. Affinity-purified acetylcholine receptors from human skeletal muscle were used in parallel in the common precipitation assay and an inhibition assay. Cross-reactivity of acetylcholine receptor antibodies was analyzed with receptor preparation from different species (calf, rat,Torpedo c. andElectrophorus e.), purified identically to high specific activity. An antibody pattern was set up for each patient and related to the clinical state of the disease. α-Bungarotoxin-inhibiting antibodies were demonstrable in 74% of myasthenia gravis patients, α-bgt displacing antibodies were found in 39% of the investigated sera. Broad cross-reactivity with acetylcholine receptors from other mammalian muscle was evident (calf 75%, rat 90%) only very few sera reacted with acetylcholine receptors from electric fish (Torpedo c. 14%,Electrophorus e. 38%). Antibody concentrations determined by using xenoantigens were much lower than those obtained by human acetylcholine receptor. The lack of a clear-cut correlation between the amount of serum antibodies and the clinical state of myasthenia gravis can be explained by the established antibody-heterogeneity, shown by a constant antibody pattern characteristic for each patient. However, between this specific antibody pattern and the state of the disease no correlation could be established either.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01716258
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Nachweis von Acetylcholin-Rezeptor-Antikörpern im Serum von Myasthenia gravis Patienten unter Verwendung affinitätschromatographisch gereinigter humaner Acetylcholin-Rezeptor-Präparationen (1979)
    Springer
    Journal of molecular medicine 57 (1979), S. 875-881 
    Details
    ISSN: 1432-1440
    Keywords: Myasthenia gravis ; Acetylcholine receptor antibodies ; Radioimmunoassay ; Clinical correlation ; Myasthenia gravis ; Azetylcholin-Rezeptor-Antikörper ; Radioimmunassay ; klinische Korrelation ; Antikörpertitration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Serum von 75 Patienten mit einer Myasthenia gravis wurden auf die Präsenz von Antikörpern gegen Azetylcholin-Rezeptoren getestet. Im Gegensatz zu vorangegangenen Experimenten wurde in den vorliegenden Untersuchungen anstelle eines Triton X-100 Skelettmuskelextraktes eine hochgereinigte Azetylcholin-Rezeptor-Präparation in dem Radioimmunassay eingesetzt, die durch Affinitätschromatographie mit α-Naja-Toxin-Sepharose 4B erhalten wurde. Wenn die gereinigte und für den Radioimmunassay mit125J-Bungaro-Toxin-Azetylcholin-Rezeptor-Präparation zum Nachweis von Serumantikörpern in einen Radioimmunassay eingesetzt wurde, fanden sich in 80% der getesteten Patienten-Seren Antikörper gegen Azetylcholin-Rezeptor-Proteine. Im Gegensatz dazu war bei keinem der Kontrollpersonen ein positiver Antikörpernachweis zu finden. Trotz der Anwendung einer gereinigten Azetylcholin-Rezeptor-Präparation konnte jedoch auch in den vorliegenden Untersuchungen keine klare Korrelation zwischen der Menge von Azetylcholin-Rezeptor-Antikörpern im Serum und dem klinischen Stadium des Patienten aufgestellt werden. Die Erstellung individueller Antikörperbindungskurven zeigte verschiedene Reaktionsmuster, die einmal durch eine verschiedene Antikörperspezifität für unterschiedliche antigene Determinanten am Rezeptormolekül oder durch eine verschiedene Affinität des Azetylcholin-Rezeptor-Antikörpers erklärt werden kann.
    Notes: Summary Sera from 75 patients with Myasthenia gravis were tested for acetylcholine receptor antibodies using acetylcholine receptors from human skeletal muscle. From the crude Triton x-100 extract, which has so far been used for antibody tracing, a pure acetylcholine receptor preparation was obtained by affinity chromatography using α-Najatoxin-Sepharose 4B. When the purified125J-α-Bungarotoxin-acetylcholine receptor complex was applied in a radioimmunoassay 80% of the Myasthenia gravis patients had acetylcholine receptor antibodies in contrast to none of the tested control persons. Inspite of using a pure acetylcholine receptor preparation, no clear-cut correlation was found between the amount of serum acetylcholine receptor antibodies and the clinical stage of the disease. When individual antibody titration curves were established, different reaction patterns were observed indicating either different antibody specificities in regard to antigenic determinants on the receptor molecule or differences in the antibody affinity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01477026
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    370. Thymektomie bei Patienten mit Myasthenia gravis (1983)
    Springer
    Langenbeck's archives of surgery 361 (1983), S. 913-913 
    Details
    ISSN: 1435-2451
    Keywords: Myastenia gravis ; Thymectomy ; Computed tomography ; Myasthenia gravis ; Thymektomie ; Computertomographie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei Patienten mit Myastenia gravis wird die Häufigkeit einer Thymushyperplasie mit 65%, von Thymustumoren mit 8–28% angegeben. Da sich selbst ein eindeutig veränderter Thymus dem klinischen Nachweis entziehen kann, hat die thorakale Computertomographie einen hohen diagnostischen Stellenwert. Die Indikation zur Thymektomie besteht bei erwachsenen Myastheniepatienten im computertomographischen Nachweis von Thymusgewebe. Für die zu fordernde komplette Entfernung des Thymusgewebes bevorzugen wir die mediane Sternotomie. Neurologische Nachbehandlung ist notwendig.
    Notes: Summary Among patients suffering from myasthenia gravis, thymic hyperplasia can be found in 65% and a tumor in 8%–28%. Clinical diagnosis can be difficult. Therefore, computed axial tomography is of great diagnostic importance. There is increasing evidence that a thymectomy should always be performed if thymic tissue can be shown by CAT scan to be present in the anterior mediastinum. As operative treatment we prefer a median sternotomy, which makes it easier to remove the thymus completely. Postoperatively the patients should be under neurological surveillance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276196
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...