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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric radiology 25 (1995), S. 19-20 
    ISSN: 1432-1998
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 10-month-old girl with acute monocytic leukemia is presented. Ultrasound of the abdomen on admission showed an unusual distribution of leukemia infiltrates in the stomach and liver. The leukemic infiltrates in the wall of the stomach presented as intraluminal polyps, while the leukemic infiltration of the liver was strictly confined to the portal vessels.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 153 (1994), S. 257-259 
    ISSN: 1432-1076
    Keywords: Lymphocyte phenotyping ; Neonatal lymphocytes Flow cytometry ; Normal values
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using flow cytometric analysis we investigated the distribution of major lymphocyte surface antigens in newborn infants. A total of 221 newborns entered the study, of whom 53 fullfilled our criteria of healthy mature neonates. Percentages of immunofluorescent-positive cells were as follows (median and range from 25th to 75th percentiles given): for CD1 3.8%; 2.3%–5.8%. CD2 60.9%; 52.4%–66.8%. CD3 57.5%; 50.5%–63.3%. TcRaß 57.7%; 48.1%–60.0%. CD4 36.3%; 28.0%–42.6%. CD8 23.0%; 20.0%–27.4%. CD11a 56.3%; 46.3%–68.5%. CD19 12.1%; 8.6%–14.8%. CD20 10.9%; 8.4%–12.9%. CD25 2.6%; 2.1%–4.5%. CDw52 61.0%; 51.2%–76.1%. CD71 5.2%; 3.1%–9.3%. While the ranges for the percentage of immunofluorescent-positive cells were rather small, there was a wide variation in the absolute numbers of marker immunofluorescent-positive cells.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 153 (1994), S. 257-259 
    ISSN: 1432-1076
    Keywords: Key words: Lymphocyte phenotyping – Neonatal lymphocytes – Flow cytometry – Normal values
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Using flow cytometric analysis we investigated the distribution of major lymphocyte surface antigens in newborn infants. A total of 221 newborns entered the study, of whom 53 fullfilled our criteria of healthy mature neonates. Percentages of immunofluorescent-positive cells were as follows (median and range from 25th to 75th percentiles given): for CD1 3.8%; 2.3% – 5.8%. CD2 60.9%; 52.4% – 66.8%. CD3 57.5%; 50.5% – 63.3%. TcRAβ 57.7%; 48.1% – 60.0%. CD4 36.3%; 28.0% – 42.6%. CD8 23.0%; 20.0% – 27.4%. CD11a 56.3%; 46.3% – 68.5%. CD19 12.1%; 8.6% – 14.8%. CD20 10.9%; 8.4% – 12.9%. CD25 2.6%; 2.1% – 4.5%. CDw 52 61.0%; 51.2% – 76.1%. CD71 5.2%; 3.1% – 9.3%. While the ranges for the percentage of immunofluorescent-positive cells were rather small, there was a wide variation in the absolute numbers of marker immunofluorescent-positive cells.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Twenty three human mononuclear cell lines including ten myelomonocytic cell lines, eight B cell lines and five T cell lines, were examined to determine whether they could be infected with dengue-2 virus. All the cell lines were infected with dengue-2 virus as determined by immunofluorescent staining and by virus titration of culture supernatant fluids. K 562, Jiyoye and Jurkat, respectively, showed the highest percentage of infected cells of these myelomonocytic, B and T cell lines. Antibody to dengue-2 virus at subneutralizing concentrations augmented dengue-2 virus infection of myelomonocytic cell lines, but not of B cell lines or of T cell lines. Persistent dengue-2 virus infection was established using a myelomonocytic cell line (K562), a B cell line (Raji), and a T cell line (HSB-2). These cell lines maintained a high percentage (more than 70%) of dengue-2 virus antigen-positive cells for at least 25 weeks. Very low titers of infectious dengue-2 virus were detected in the culture supernatant fluids of the persistently infected cells. Dengue-2 virus antigen-positive Raji cell clones were established from persistently-infected Raji cells using limiting dilutions and all of the cells in these clones were dengue-2 virus antigen-positive. These findings demonstrate that a variety of human mononuclear cell lines can be infected with dengue-2 virus and may be useful as models for the analysis of dengue virus-human cell interactions in dengue virus infections.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Infection 21 (1993), S. 89-92 
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Inzidenz von ZNS-Shuntinfektionen und damit verbundenen Risikofaktoren wurde durch Auswertung von Krankenakten untersucht. Zwischen 1986 und 1989 wurden 350 Shunt-Operationen durchgeführt, wobei 273 Shunts ventrikuloperitoneal und 75 ventrikuloatrial plaziert wurden. Bei 25 Patienten kam es während eines mittleren Beobachtungszeitraumes von 20 Monaten zu 28 Shuntinfektionen (8 %). Für 204 Patienten konnte die Nachbeobachtungszeit bis September 1992 ausgedehnt werden, weitere Infektionsfälle wurden in dieser Gruppe nicht beobachtet. In 24 Fällen (85,7 %) konnte ein ursächlicher Erreger isoliert werden. Bei den Erregern handelte es sich um grampositive Kokken in 22 Fällen (78,6 %) und um gramnegative Stäbchen in zwei Fällen. Hauptsymptome und -Befunde waren Fieber, Shuntdysfunktion und meningeale Reizerscheinungen sowie Bauchschmerzen (letztere ausschließlich bei VP-Shunts). Bei 24 Infektionsfällen wurde der Shunt sofort entfernt und eine antibiotische Therapie begonnen, in den übrigen Fällen erfolgte ausschließlich eine antibiotische Therapie. Ursache des Hydrozephalus, Shuntlage, perioperative Antibiotikagabe, Alter und Geschlecht eines Patienten waren nicht mit einem erhöhten Infektionsrisiko assiziiert, jedoch ergab sich ein Trend, der ein erhöhtes Infektionsrisiko mit der Operationsdauer zeigte. Die Infektionsrate lag bei 13,6% für eine Operationsdauer von 〉90 Minuten, während sie bei 5,2 % für Eingriffe mit einer Dauer von 〈30 Minuten lag.
    Notes: Summary The incidence of shunt infections and possible risk factors was investigated by chart analysis. From 1986 to 1989 350 shunt procedures were performed including 273 ventriculoperitoneal shunts and 75 ventriculoatrial shunts. Twenty-eight infectious episodes (8%) occurred in 25 patients during a median follow-up time of 20 months. For 204 patients the follow-up time could be prolonged until September 1992. In these patients no infectious episodes occurred in the extended observation period. In 24 cases (85.7%) a causative organism could be isolated. The infecting organisms were gram-positive cocci in 22 cases (78.6%) and gram-negative bacilli in two cases. The main signs and symptoms were fever, shunt malfunction and meningeal irritation, and with VP-shunts only, abdominal pain. Twenty-four infectious episodes were treated with antibiotics and immediate removal of the shunt. The remaining were managed with antibiotics only. The risk for shunt infection did not correlate with age or sex of patients, nor with the etiology of hydrocephalus, type of shunt implanted or perioperative antibiotic prophylaxis. However, a trend showing a higher risk for shunt infections with prolonged operation time was noticed. The infection rate was 13.6% for an operation lasting more than 90 minutes versus 5.2% for procedures of less than 30 minutes' duration.
    Type of Medium: Electronic Resource
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