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  • 1
    Electronic Resource
    Electronic Resource
    Topoisomerase IIα gene (TOP2A) amplification and deletion in cancer – more common than anticipated (2003)
    Oxford, UK : Blackwell Science Ltd
    Cytopathology 14 (2003), S. 0 
    Details
    ISSN: 1365-2303
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In solid tumours the predominant genetic mechanism for oncogene activation is through amplification of genes. The HER-2 (also known as ErbB2/c-erbB2/HER-2/neu) oncogene is the most frequently amplified oncogene in breast cancer and is also commonly amplified in other forms of cancer. The HER-2 amplicon also contains other biologically relevant genes with altered copy numbers, among these genes is the topoisomerase IIα (TOP2A). TOP2A gene is located adjacent to the HER-2 oncogene at the chromosome location 17q12-q21 and is either amplified or deleted, with equal frequency, in almost 90% of HER-2 amplified primary breast tumours. Recent data suggest that amplification and deletion of TOP2A may account for both sensitivity and resistance to topoII-inhibitor-chemotherapy, depending on the specific genetic defect at the TOP2A locus.In this issue of the Cytopathology, Bofin et al. present preliminary evidence for high prevalance of TOP2A amplification and deletion not only in the HER-2 amplified breast tumours, but also in the primary breast tumours without the HER-2 amplification. This finding together with the concept that TOP2A gene amplification and deletion seem to account for both relative chemosensitivity and resistance to topoII-inhibitor therapy further highlights the importance of screening for TOP2A gene copy number aberrations when topoII-inhibitors are considered either alone or in combination of other chemotherapeutic drugs for the treatment of cancer patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0956-5507.2003.00105.x
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of allelic loss within the β1-interferon gene in childhood acute lymphoblastic leukemia using differential PCR (1994)
    Springer
    Annals of hematology 68 (1994), S. 171-174 
    Details
    ISSN: 1432-0584
    Keywords: Differential PCR ; Β1-IFN gene loss ; Acute lymphoblastic leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Deletion of the short arm of chromosome 9p involving the Β1-interferon (IFN) gene has been implicated in the process of malignant transformation in lymphomas and acute lymphoblastic leukemias. Since cytogenetic analysis is frequently unsuccessful in clinical samples, we used a recently described differential PCR technique to detect losses within the Β1-IFN gene in 86 acute leukemias. Using differential PCR, no Β1-IFN deletion was detected in 44 acute myeloid leukemia (AML) and eight control samples. However, five of 42 acute lymphoblastic leukemia (ALL) probes (12%) exhibited loss of the Β1-IFN gene (three common ALL, two T-ALL). Cytogenetic analysis was performed independently in three of these five cases and revealed abnormalities of chromosome 9p in two samples. Two of five T-ALL cases exhibited a loss within the Β1-IFN gene, compared with 3/29 c-ALLs, suggesting a predominance of IFN gene loss in T-ALLs. These data indicate that PCR can be used for rapid detection of gene dosage phenomena in clinical leukemia samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01834362
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    Paper (German National Licenses)
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