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  • 1
    Electronic Resource
    Electronic Resource
    Effect of triiodothyronine on system A amino acid transport in cells of rat submandibular gland (1987)
    Springer
    Pflügers Archiv 408 (1987), S. 519-523 
    Details
    ISSN: 1432-2013
    Keywords: NaK-ATPase ; α-Aminoisobutyric acid ; Triiodothyronine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect ofL-3,5,3′-triiodothyronine (T3) on α-aminoisobutyric acid (AIB) transport in isolated cell suspensions of rat submandibular gland was investigated. The uptake of ATB by these cells appeared to require extracellular Na+ and was inhibited by ouabain (10−3 M). Cell suspensions from thyroidectomized rats which have been given three successive doses of T3 on alternate days (50 μg/100 g BW) showed a significant increase in AIB uptake compared with cells isolated from thyroidectomized rats treated with diluent. Efflux of AIB from the cell suspension was not affected by T3. There was no significant changes in AIB uptake 12 h after a single injection of T3 (50 μg/100 g BW). However, there was a significant 49% and 65% increase in AIB net uptake at 24 and 48 h, respectively, after T3 treatment. Under similar conditions, the cell suspension showed a 48% increase in NaK-ATPase activity at 12 h and to a peak of 61% at 24 h. Therefore, changes in NaK-ATPase activity preceded the changes in AIB net uptake upon treatment with T3, implying that AIB uptake is probably mediated, at least in part, by the activity of NaK-ATPase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585079
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of corticosterone and triiodothyronine on (Na++K+)-adenosine triphosphatase activity in rat submandibular gland (1983)
    Springer
    Pflügers Archiv 399 (1983), S. 255-258 
    Details
    ISSN: 1432-2013
    Keywords: Corticosterone ; Dexamethasone ; Triiodothyronine ; Thyroidadrenalectomy ; Adrenalectomy ; NaK-ATPase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study concerns the effect of glucocorticoids and triiodothyronine (T3) on rat salivary (Na++K+)-adenosine triphosphatase (NaK-ATPase). The results indicate that corticosterone increases submandibular gland NaK-ATPase activity with a single dose of hormone and with two daily doses on three successive days. Multiple injections of dexamethasone also increases submandibular and sublingual gland NaK-ATPase activity. The effect of glucocorticoids on salivary NaK-ATPase is selective since Mg-ATPase shows no significant differences under different steroidal states. We subsequently investigate the possibility of interaction between the effects of corticosterone and triiodothyronine on submandibular gland NaK-ATPase activity. Our studies indicate that each of these hormones increases NaK-ATPase activity in the absence of the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00652748
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Neomycin inhibits mastoparan-induced lactate dehydrogenase release, ethidium bromide accumulation, and intracellular fluorescein depletion in MDCK cells (1990)
    Springer
    Cell biology and toxicology 6 (1990), S. 95-104 
    Details
    ISSN: 1573-6822
    Keywords: Mastoparan ; Lactate Dehydrogenase ; Neomycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to perturb membrane phospholipids resulting in an increase in membrane permeability, we studied the effect of chemically synthesized mastoparan on lactate dehydrogenase release (LDH), ethidium bromide and fluorescein accumulation in Madin-Darby Canine Kidney (MDCK) cultured cells. Our results demonstrated that mastoparan induced cytosolic LDH release, ethidium bromide accumulation and intracellular fluorescein depletion in MDCK cells. Neomycin, a polycationic aminoglycoside, interacts with anionic polyphosphoinositides at the plasma membrane. Since both mastoparan and neomycin are cationic peptides and react with the negatively charged membrane phospholipids, we studied the interaction of these two peptides on membrane permeability. Our results demonstrated that neomycin inhibited mastoparan-induced LDH release, ethidium bromide accumulation and intracellular fluorescein depletion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00135029
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    Paper (German National Licenses)
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