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  • 1
    Electronic Resource
    Electronic Resource
    Fatty acid patterns of Acinetobacter calcoaceticus 69-V indicate sensitivity against xenobiotics (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 526-531 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The growth of Acinetobacter calcoaceticus 69-V on the alcohols ethanol, n-propanol, n-pentanol, n-hexanol and on phenol caused an alteration in its fatty acid composition leading to a gradual increase in the degree of saturation of the C16 acids from 55.4% to 83.5%, which (apart from phenol-grown cells) correlated to an increase in the resistance of the electron-transport phosphorylation against the effect of 2,4-dinitrophenol. These changes are in principle paralleled by changes observed when the growth temperature was increased in the sequence 20, 30 and 40°C with acetate as the carbon and energy source. However, in cells grown at 40°C, as in the case of phenol-grown cells, resistance decreased. This effect could be caused by an increase in the fluidity of the target membrane since, by contrast, the increase in sensitivity induced by growth at 40°C can be partially annulled by provoking a decrease in fluidity by performing the inhibition measurements at a lower temperature (20°C). Both the degree of saturation of the fatty acids and the fluidity of the cytoplasmic membrane are features that should enable the resistance of Acinetobacter calcoaceticus 69-V to xenobiotics to be predicted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050593
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The glucose dehydrogenase-mediated energization of Acinetobacter calcoaceticus as a tool for evaluating its susceptibility to, and defence against, hazardous chemicals (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 738-743 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cells of Acinetobacter calcoaceticus 69-V could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. The velocities of glucose oxidation-driven ATP syntheses were relatively constant in the range from pH 5.4 to 7.5. With decreasing pH values (7.0, 6.0, 5.4) ATP synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same pH value glucose oxidation was nearly unimpaired or inhibited more weakly. This finding is expressed by a decrease of the P/O ratios, indicating the uncoupling of the electron-transport phosphorylation by 2,4-dinitrophenol. The sensitivity towards this uncoupling effect was higher in ethanol-grown cells of Acinetobacter calcoaceticus 69-V than in hexanol- or acetate-grown cells. This increase in sensitivity was accompanied by a decrease of the ratio of saturated (mainly C16:0) to unsaturated (C16:1, C18:1) fatty acids in ethanol-grown cells compared with hexanol-grown ones. The knowledge of such differences in the susceptibility and its molecular background, e.g. possible substrate-induced changes of the fatty acid composition of the cytoplasmic membranes, should help elucidate mechanisms of poisoning by membrane-active hazardous chemicals and develop defence strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171955
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Fatty acid patterns of Acinetobacter calcoaceticus 69-V indicate sensitivity against xenobiotics (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 526-531 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The growth of Acinetobacter calcoaceticus 69-V on the alcohols ethanol, n-propanol, n-pentanol, n-hexanol and on phenol caused an alteration in its fatty acid composition leading to a gradual increase in the degree of saturation of the C16 acids from 55.4% to 83.5%, which (apart from phenol-grown cells) correlated to an increase in the resistance of the electron-transport phosphorylation against the effect of 2,4-dinitrophenol. These changes are in principle paralleled by changes observed when the growth temperature was increased in the sequence 20, 30 and 40°C with acetate as the carbon and energy source. However, in cells grown at 40°C, as in the case of phenol-grown cells, resistance decreased. This effect could be caused by an increase in the fluidity of the target membrane since, by contrast, the increase in sensitivity induced by growth at 40°C can be partially annulled by provoking a decrease in fluidity by performing the inhibition measurements at a lower temperature (20°C). Both the degree of saturation of the fatty acids and the fluidity of the cytoplasmic membrane are features that should enable the resistance of Acinetobacter calcoaceticus 69-V to xenobiotics to be predicted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169955
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The glucose dehydrogenase-mediated energization of Acinetobacter calcoaceticusas a tool for evaluating its susceptibility to, and defence against, hazardous chemicals (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 738-743 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cells of Acinetobacter calcoaceticus 69-V could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. The velocities of glucose oxidation-driven ATP syntheses were relatively constant in the range from pH 5.4 to 7.5. With decreasing pH values (7.0, 6.0, 5.4) ATP synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same pH value glucose oxidation was nearly unimpaired or inhibited more weakly. This finding is expressed by a decrease of the P/O ratios, indicating the uncoupling of the electron-transport phosphorylation by 2,4-dinitrophenol. The sensitivity towards this uncoupling effect was higher in ethanol-grown cells of Acinetobacter calcoaceticus 69-V than in hexanol- or acetate-grown cells. This increase in sensitivity was accompanied by a decrease of the ratio of saturated (mainly C16:0) to unsaturated (C16:1, C18:1) fatty acids in ethanol-grown cells compared with hexanol-grown ones. The knowledge of such differences in the susceptibility and its molecular background, e.g. possible substrate-induced changes of the fatty acid composition of the cytoplasmic membranes, should help elucidate mechanisms of poisoning by membrane-active hazardous chemicals and develop defence strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050324
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    The influence of energy deficiency-imposing conditions on the capacities ofAcetobacter methanolicus to oxidize glucose and to produce gluconic acid (1993)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 13 (1993), S. 251-256 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The presence of dinitrophenol (DNP) during the chemostatic growth of Acetobacter methanolicuson glucose led to i) no significant increase in glucose dehydrogenase, ii) a decrease in the capacity to oxidize glucose by about 30%, not depending on the DNP concentration, and iii) a gradual decrease in the capacity to form gluconic acid, depending on the concentration of the uncoupler. Similar effects of DNP on Acetobacter methanolicuswere observed during the gluconic acid formation process. The loss in the capacity to form gluconic acid is indicated by a drop in the ATP concentration of the cells which cannot be counteracted by the ATP syntheses originating from the oxidation of gluconic acid and of glucose. ATP could be necessary for pumping protons out of the cells that were taken up together with gluconic acid and by the effect of DNP.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370130305
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of cytochrome composition on the energy conservation of acinetobacter calcoaceticus 69-V (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 551-560 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Acinetobacter calcoaceticus 69-V contained cytochromes b, o, d and traces of cytochrome a1 after growth on ethanol. Cytochromes o, d, and a1 are known to be potentially capable of functioning as terminal oxidases. After growth on acetate only smaller amounts of cytochromes b and o were detectable. Cytochrome spectra of acetate plus glucose-grown cells resembled more those of ethanolgrown cells. Cytochrome spectra of acetate plus ethanol-grown cells were more similar to those of acetate-grown cells but already showed small amounts of cytochrome d.Ethanol-grown cells were energized only by the oxidation of glucose. But the velocity of ATP synthesis was so low that the P/O quotient amounted to practically zero. The stoichiometries of ATP synthesis of the otherwise-grown cells were generally higher for the oxidation of glucose than for the oxidations of succinate and D-lactate. Similar high P/O quotients were measured for the oxidations of glucose and ethanol in acetate plus ethanol-grown cells.No significant energization could be observed during the oxidations of gluconate and acetate.The findings indicate that electrons delivered by the PQQ-dependent glucose as well as by NAD+- dependent ethanol oxidations have probably a common place of entry into the respiratory chain. Glucose and NADH appeared to be energetically equivalent although at least two energetically different pathways might be involved in substrate oxidation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100617
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    Paper (German National Licenses)
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