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  • 1
    Electronic Resource
    Electronic Resource
    Fatty acid patterns of Acinetobacter calcoaceticus 69-V indicate sensitivity against xenobiotics (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 526-531 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The growth of Acinetobacter calcoaceticus 69-V on the alcohols ethanol, n-propanol, n-pentanol, n-hexanol and on phenol caused an alteration in its fatty acid composition leading to a gradual increase in the degree of saturation of the C16 acids from 55.4% to 83.5%, which (apart from phenol-grown cells) correlated to an increase in the resistance of the electron-transport phosphorylation against the effect of 2,4-dinitrophenol. These changes are in principle paralleled by changes observed when the growth temperature was increased in the sequence 20, 30 and 40°C with acetate as the carbon and energy source. However, in cells grown at 40°C, as in the case of phenol-grown cells, resistance decreased. This effect could be caused by an increase in the fluidity of the target membrane since, by contrast, the increase in sensitivity induced by growth at 40°C can be partially annulled by provoking a decrease in fluidity by performing the inhibition measurements at a lower temperature (20°C). Both the degree of saturation of the fatty acids and the fluidity of the cytoplasmic membrane are features that should enable the resistance of Acinetobacter calcoaceticus 69-V to xenobiotics to be predicted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050593
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  • 2
    Electronic Resource
    Electronic Resource
    Fatty acid patterns of Acinetobacter calcoaceticus 69-V indicate sensitivity against xenobiotics (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 526-531 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The growth of Acinetobacter calcoaceticus 69-V on the alcohols ethanol, n-propanol, n-pentanol, n-hexanol and on phenol caused an alteration in its fatty acid composition leading to a gradual increase in the degree of saturation of the C16 acids from 55.4% to 83.5%, which (apart from phenol-grown cells) correlated to an increase in the resistance of the electron-transport phosphorylation against the effect of 2,4-dinitrophenol. These changes are in principle paralleled by changes observed when the growth temperature was increased in the sequence 20, 30 and 40°C with acetate as the carbon and energy source. However, in cells grown at 40°C, as in the case of phenol-grown cells, resistance decreased. This effect could be caused by an increase in the fluidity of the target membrane since, by contrast, the increase in sensitivity induced by growth at 40°C can be partially annulled by provoking a decrease in fluidity by performing the inhibition measurements at a lower temperature (20°C). Both the degree of saturation of the fatty acids and the fluidity of the cytoplasmic membrane are features that should enable the resistance of Acinetobacter calcoaceticus 69-V to xenobiotics to be predicted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169955
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  • 3
    Electronic Resource
    Electronic Resource
    The glucose dehydrogenase-mediated energization of Acinetobacter calcoaceticus as a tool for evaluating its susceptibility to, and defence against, hazardous chemicals (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 738-743 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cells of Acinetobacter calcoaceticus 69-V could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. The velocities of glucose oxidation-driven ATP syntheses were relatively constant in the range from pH 5.4 to 7.5. With decreasing pH values (7.0, 6.0, 5.4) ATP synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same pH value glucose oxidation was nearly unimpaired or inhibited more weakly. This finding is expressed by a decrease of the P/O ratios, indicating the uncoupling of the electron-transport phosphorylation by 2,4-dinitrophenol. The sensitivity towards this uncoupling effect was higher in ethanol-grown cells of Acinetobacter calcoaceticus 69-V than in hexanol- or acetate-grown cells. This increase in sensitivity was accompanied by a decrease of the ratio of saturated (mainly C16:0) to unsaturated (C16:1, C18:1) fatty acids in ethanol-grown cells compared with hexanol-grown ones. The knowledge of such differences in the susceptibility and its molecular background, e.g. possible substrate-induced changes of the fatty acid composition of the cytoplasmic membranes, should help elucidate mechanisms of poisoning by membrane-active hazardous chemicals and develop defence strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171955
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  • 4
    Electronic Resource
    Electronic Resource
    The glucose dehydrogenase-mediated energization of Acinetobacter calcoaceticusas a tool for evaluating its susceptibility to, and defence against, hazardous chemicals (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 738-743 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cells of Acinetobacter calcoaceticus 69-V could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. The velocities of glucose oxidation-driven ATP syntheses were relatively constant in the range from pH 5.4 to 7.5. With decreasing pH values (7.0, 6.0, 5.4) ATP synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same pH value glucose oxidation was nearly unimpaired or inhibited more weakly. This finding is expressed by a decrease of the P/O ratios, indicating the uncoupling of the electron-transport phosphorylation by 2,4-dinitrophenol. The sensitivity towards this uncoupling effect was higher in ethanol-grown cells of Acinetobacter calcoaceticus 69-V than in hexanol- or acetate-grown cells. This increase in sensitivity was accompanied by a decrease of the ratio of saturated (mainly C16:0) to unsaturated (C16:1, C18:1) fatty acids in ethanol-grown cells compared with hexanol-grown ones. The knowledge of such differences in the susceptibility and its molecular background, e.g. possible substrate-induced changes of the fatty acid composition of the cytoplasmic membranes, should help elucidate mechanisms of poisoning by membrane-active hazardous chemicals and develop defence strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050324
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  • 5
    Electronic Resource
    Electronic Resource
    Influence of crude oil contamination on the bacterial community of semiarid soils of Patagonia (Argentina) (2000)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 20 (2000), S. 129-146 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Autochthonous bacteriocenoses in semiarid soils in Patagonia were found to be capable of rapidly adapting to high contamination with crude oil. This adaptation at community level is due to the selective enrichment of hydrocarbon-utilizing bacteria always present in these soils. Immediately after a heavy contamination with crude oil, the authochthonous bacteriocenosis contained about 28% hydrocarbon-utilizing bacteria which could be classified into eight ecotypes with characteristic metabolic profiles. Mainly n-alkanes were used as growth substrates of representative strains. After seven months' exposure to crude oil, the bacteriocenosis consisted almost entirely of hydrocarbon-utilizing bacteria. At least fourteen ecotypes were distinguishable, and the majority of representative strains were able to metabolize a broad spectrum of aliphatic and aromatic hydrocarbons. Corresponding to the significant alteration of the physiological diversity, drastic changes to the taxonomic diversity were also found. Whereas at the beginning of the study the autochthonous bacteriocenoses were dominated by GRAM-positive genera of the Actinomycetales (Dietzia, Gordona, Nocardia, Rhodococcus, Streptomyces) with high ecological potency, after just two months' exposure to crude oil, GRAM- negative bacteria (especially Pseudomonas stutzeri) became predominant within the hydrocarbon-utilizing bacteriocenoses accompanied by some GRAM-positive genera of the Actinomycetales with a significantly lower abundance. These findings underline the importance of Pseudomonas and some genera of Actinomycetales for processes of natural attenuation and the technically supported in situ bioremediation of soil polluted by crude oil in Patagonia.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370200207
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  • 6
    Electronic Resource
    Electronic Resource
    Phenomenological principles of microbial lignin degradation (1993)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 13 (1993), S. 31-40 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper intends to focus the attention to characteristic features of microbial lignin degradation from the phenomenological point of view. Six fundamental principles are discussed under special consideration of white-rot fungi. The necessity of mycelial growth and the formation, secretion, and extracellular action of peroxidases are main requirements for a successful microbial attack on polymeric lignin.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370130107
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  • 7
    Electronic Resource
    Electronic Resource
    Biochemisch-mikrobiologisches Praktikum. Stuttgart, New York: Georg Thieme Verlag, 1987,409 S., DM 39,80, ISBN 3-13-685901-4 (1989)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 346-346 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370090407
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Zur Existenz des Oxoniumions in festen basischen Aluminiumsulfaten (1986)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 542 (1986), S. 201-206 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: On the Existence of the Oxonium Ion in Solid Basic Aluminium SulphatesOxonium alunite was investigated by 1H-NMR-, heat capacity, electric conductivity, and thermoanalytic measurements. In the 1H-NMR spectrum the existence of the oxonium ion was surely proved. From the results of the other investigations follow, that the oxonium ion is fast bonded in the structure and that exists an unimportant amount of mobile ionic charge carriers only.
    Notes: Oxoniumalunit wurde mittels 1H-NMR-, Wärmekapazitäts-, elektrischer Leitfähigkeits- und thermoanalytischer Messungen untersucht. Das 1H-NMR-Spektrum weist eindeutig die Existenz des Oxoniumions aus. Die Ergebnisse der anderen Untersuchungen zeigen, daß das Oxoniumion in der Struktur fest gebunden ist und daß nur sehr wenige verschiebbare ionische Ladungsträger vorhanden sind.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19865421126
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  • 9
    Electronic Resource
    Electronic Resource
    Fe—Al-Isomorphie und Strukturwasser in Kristallen vom Jarosit-Alunit-Typ (1984)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 508 (1984), S. 159-164 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Fe—Al-Isomorphism and Structural Water in Crystals of Jarosite-Alunite-TypeThe isomorphous relation between synthetic Alunite KAl3(OH)6(SO4)2 and Jarosite KFe3(OH)6(SO4)2 is investigated. Chemical analysis, X-ray diffraction and thermal analysis permit to draw conclusions on the stoichiometry of the solid solution. It can be shown that under the chosen synthesis conditions the following results are obtainable: Iron is preferably built in the crystal lattice of the solid solution.There are vacancies on the octahedral positions of aluminium or iron.There is always an excess of water in the structure.Because of the lacking Fe3+/Al3+ charges it seems possible that a partial OH-—H2O substitution exists in the structure.
    Notes: Die Isomorphiebeziehung zwischen synthetischem Alunit KAl3(OH)6(SO4)2 und Jarosit KFe3(OH)6(SO4)2 wird untersucht. Aus den Ergebnissen der chemischen Analyse, Röntgenbeugung und thermischen Analyse werden Aussagen zur Stöchiometrie der gebildeten Mischkristalle abgeleitet. Unter den gewählten Synthesebedingungen konnten folgende Resultate erhalten werden: Eisen wird stark bevorzugt in das Kristallgitter der Mischkristalle eingebaut.Charakteristisch für die synthetisierten Verbindungen ist ein Defizit an dreiwertigen Ionen. Das führt zu der Schlußfolgerung, daß partiell Aluminium- bzw. Eisen-Oktaederpositionen als Leerstellen ausgebildet sein müssen.Es wird stets ein Überschuß an Wasser in der Struktur gefunden. Auf Grund des Defizit an Fe3+/Al3+ ist eine teilweise OH--Substitution durch H2O in der Struktur zu diskutieren.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19845080123
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