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  • 1
    Electronic Resource
    Electronic Resource
    Correlation between cellular fluorescence and NAD(P)H levels in Alcaligenes eutrophus JMP 134 (1996)
    Springer
    Bioprocess and biosystems engineering 14 (1996), S. 57-62 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The culture fluorescence of Alcaligenes eutrophus JMP 134 was determined on-line by an Ingold Fluorosensor™ and correlated to the intracellular concentrations of reduced nicotinamide adenine dinucleotide (phosphate). The data were obtained from aerobic cultures of the strain growing chemostatically on phenol, phenol+sodium formate and fructose, as well as from aerobic/anaerobic transitions and substrate pulse experiments. The total culture fluorescence was corrected to take into account the inner filter effect of cells. Upon analysing the intracellular concentration of the dinucleotides using HPLC, it became evident that both NADH and NADPH contribute significantly to the fluorescence signal. A linear relationship between the sum of NAD(P)H and the net culture fluorescence was obtained from these data with a correlation factor of r=0.82. These investigations indicate that the measurement of culture fluorescence is a practicable tool for monitoring the redox state of a cellular culture, provided the total fluorescence signal is adjusted and the investigations are supported by direct measurements of intracellular levels of reduced dinucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387958
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  • 2
    Electronic Resource
    Electronic Resource
    The linear oxidation of formaldehyde to CO2 as the proper energy generating sequence for the assimilation of methanol in Acetobacter methanolicus MB58 (1987)
    Springer
    Archives of microbiology 149 (1987), S. 149-155 
    Details
    ISSN: 1432-072X
    Keywords: Acetobacter methanolicus MB58 ; Methylotrophy ; Linear and cyclic dissimilatory sequence of formaldehyde ; Energy generation ; Mutants ; Regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acetobacter methanolicus MB58 can grow on methanol. Since this substrate exhibits to be energy deficient there must be a chance to oxidize methanol to CO2 merely for purpose of energy generation. For the assimilation of methanol the FBP variant of the RuMP pathway is used. Hence methanol can be oxidized cyclically via 6-phosphogluconate. Since Acetobacter methanolicus MB58 possesses all enzymes for a linear oxidation via formate the question arises which of both sequences is responsible for generation of the energy required. In order to clarify this the linear sequence was blocked by inhibiting the formate dehydrogenase with hypophosphite and by mutagenesis inducing mutants defective in formaldehyde or formate dehydrogenase. It has been shown that the linear dissimilatory sequence is indispensable for methylotrophic growth. Although the cyclic oxidation of formaldehyde to CO2 has not been influenced by hypophosphite and with mutants both the wild type and the formaldehyde dehydrogenase defect mutants cannot grown on methanol. The cyclic oxidation of formaldehyde does not seem to be coupled to a sufficient energy generation, probably it operates only detoxifying and provides reducing equivalents for syntheses. The regulation between assimilation and dissimilation of formaldehyde in Acetobacter methanolicus MB58 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425081
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  • 3
    Electronic Resource
    Electronic Resource
    Glucose stimulates a decrease of the fatty acid saturation degree in Acinetobacter calcoaceticus (1999)
    Springer
    Archives of microbiology 171 (1999), S. 166-172 
    Details
    ISSN: 1432-072X
    Keywords: Key words Fatty acids ; Temperature adaptation ; Acinetobacter calcoaceticus ; Fatty acid desaturase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fatty acid composition of Acinetobacter calcoaceticus 69-V was determined under various growth conditions. Saturated, unsaturated, and hydroxy fatty acids with chain lengths of 12–18 carbon atoms predominated in the fatty acid profile. With acetate or propanol as growth substrates, the ratio of saturated to unsaturated fatty acids varied with changes in the temperature. This was the only adaptive mechanism detected that compensated for the physical effects of temperature alterations on the cell membranes. The fatty acid composition of A. calcoaceticus grown at 40 °C had a saturation degree of approximately 50%; after growth at 20 °C it was approximately 35%. In the presence of a carbon and energy source, A. calcoaceticus was able to respond to temperature reductions under oxic conditions regardless of whether fatty acid biosynthesis was inhibited or not. This suggests an aerobic mechanism of fatty acid biosynthesis and the involvement of a fatty acid desaturase system. Addition of the non-growth substrate, glucose, helped the organism to adapt to lower temperature. The molecular mechanism of the aid is not really understood. The oxidation of glucose could provide the desaturase either with electrons directly via a pyrrolo-quinoline-quinone-linked glucose dehydrogenase or with NADH after fatty acid degradation has been initiated by ATP generated by the oxidation of glucose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050695
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  • 4
    Electronic Resource
    Electronic Resource
    The relation between the assimilation of methanol and glycerol in yeasts (1982)
    Springer
    Archives of microbiology 132 (1982), S. 179-184 
    Details
    ISSN: 1432-072X
    Keywords: Assimilation ; Methanol ; Glycerol ; Yeasts ; Dihydroxyacetone kinase ; Regulatory properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fifteen yeast strains of the genera Candida, Lodderomyces, Endomycopsis, Saccharomyces, Hansenula, Pichia and Torulopsis were investigated with respect to their ability to grow on methanol, glycerol and glucose as sole carbon and energy source. Eight of them can grow on both methanol and glycerol. Methanol is assimilated via triosephosphate (dihydroxyacetone) pathway. The dihydroxyacetone kinase is a key enzyme in methanol metabolism. The assimilation of glycerol can take place in bacteria via a phosphorylative or/and oxidative pathways. In general, the phosphorylative pathway is found in eucaryotes. In the present paper it is shown that in yeasts, which can utilize methanol and glycerol, too, glycerol is assimilated via an oxidative pathway, Dihydroxyacetone is a central intermediate in the assimilation of methanol as well as glycerol. It is metabolized by means of the dihydroxyacetone kinase. The enzyme formed during growth of Candida methylica on methanol does not differ from that of Candida valida H 122 after growing on glycerol as far as the regulatory properties are concerned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508727
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  • 5
    Electronic Resource
    Electronic Resource
    Amino acid sequence determination of the ADP,ATP carrier from beef heart mitochondria - The sequence of the C-terminal acidolytic fragment
    Amsterdam : Elsevier
    BBA - Protein Structure 670 (1981), S. 176-180 
    Details
    ISSN: 0005-2795
    Keywords: (Beef heart mitochondria) ; ADP,ATP carrier ; Acidolytic fragment ; C-terminal sequence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2795(81)90006-4
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  • 6
    Electronic Resource
    Electronic Resource
    The conversion of triosephosphate via methylglyoxal, a bypass to the glycolytic sequence in methylotrophic yeasts? (1981)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 10 (1981), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1981.tb06223.x
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation of ^3H-labelled bongkrekate
    Amsterdam : Elsevier
    FEBS Letters 61 (1976), S. 124-127 
    Details
    ISSN: 0014-5793
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(76)81018-6
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  • 8
    Electronic Resource
    Electronic Resource
    Dynamics of yeast cell states during proliferation and non proliferation periods in a brewing reactor monitored by multidimensional flow cytometry (1997)
    Springer
    Bioprocess engineering 17 (1997), S. 287-293 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Current demands of the brewing industry require that increasing amounts of beer be produced in ever-decreasing times, without prejudicing the quality of the product. The four basic ingredients for brewing beer are malt, hops, water and yeast. Of these four materials, yeast is unique, in that its competent handling can reduce the time taken for the brewing process. For this purpose, it is necessary to have information regarding the metabolic acting as well as the physiological state of an individual cell for which flow cytometry is used. Knowledge of changes in DNA, neutral lipid and 3β-hydroxysterol content of the yeast cells during growth, fermentation and storage enables for a time-saving process control. All of these parameters were conveniently monitored by flow cytometry in conjunction with double fluorescent staining techniques, as shown in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004490050387
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  • 9
    Electronic Resource
    Electronic Resource
    Fatty acid patterns of Acinetobacter calcoaceticus 69-V indicate sensitivity against xenobiotics (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 526-531 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The growth of Acinetobacter calcoaceticus 69-V on the alcohols ethanol, n-propanol, n-pentanol, n-hexanol and on phenol caused an alteration in its fatty acid composition leading to a gradual increase in the degree of saturation of the C16 acids from 55.4% to 83.5%, which (apart from phenol-grown cells) correlated to an increase in the resistance of the electron-transport phosphorylation against the effect of 2,4-dinitrophenol. These changes are in principle paralleled by changes observed when the growth temperature was increased in the sequence 20, 30 and 40°C with acetate as the carbon and energy source. However, in cells grown at 40°C, as in the case of phenol-grown cells, resistance decreased. This effect could be caused by an increase in the fluidity of the target membrane since, by contrast, the increase in sensitivity induced by growth at 40°C can be partially annulled by provoking a decrease in fluidity by performing the inhibition measurements at a lower temperature (20°C). Both the degree of saturation of the fatty acids and the fluidity of the cytoplasmic membrane are features that should enable the resistance of Acinetobacter calcoaceticus 69-V to xenobiotics to be predicted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050593
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  • 10
    Electronic Resource
    Electronic Resource
    Fatty acid patterns of Acinetobacter calcoaceticus 69-V indicate sensitivity against xenobiotics (1995)
    Springer
    Applied microbiology and biotechnology 44 (1995), S. 526-531 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The growth of Acinetobacter calcoaceticus 69-V on the alcohols ethanol, n-propanol, n-pentanol, n-hexanol and on phenol caused an alteration in its fatty acid composition leading to a gradual increase in the degree of saturation of the C16 acids from 55.4% to 83.5%, which (apart from phenol-grown cells) correlated to an increase in the resistance of the electron-transport phosphorylation against the effect of 2,4-dinitrophenol. These changes are in principle paralleled by changes observed when the growth temperature was increased in the sequence 20, 30 and 40°C with acetate as the carbon and energy source. However, in cells grown at 40°C, as in the case of phenol-grown cells, resistance decreased. This effect could be caused by an increase in the fluidity of the target membrane since, by contrast, the increase in sensitivity induced by growth at 40°C can be partially annulled by provoking a decrease in fluidity by performing the inhibition measurements at a lower temperature (20°C). Both the degree of saturation of the fatty acids and the fluidity of the cytoplasmic membrane are features that should enable the resistance of Acinetobacter calcoaceticus 69-V to xenobiotics to be predicted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169955
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    Paper (German National Licenses)
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