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  • 1
    Electronic Resource
    Electronic Resource
    Correlation between cellular fluorescence and NAD(P)H levels in Alcaligenes eutrophus JMP 134 (1996)
    Springer
    Bioprocess and biosystems engineering 14 (1996), S. 57-62 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The culture fluorescence of Alcaligenes eutrophus JMP 134 was determined on-line by an Ingold Fluorosensor™ and correlated to the intracellular concentrations of reduced nicotinamide adenine dinucleotide (phosphate). The data were obtained from aerobic cultures of the strain growing chemostatically on phenol, phenol+sodium formate and fructose, as well as from aerobic/anaerobic transitions and substrate pulse experiments. The total culture fluorescence was corrected to take into account the inner filter effect of cells. Upon analysing the intracellular concentration of the dinucleotides using HPLC, it became evident that both NADH and NADPH contribute significantly to the fluorescence signal. A linear relationship between the sum of NAD(P)H and the net culture fluorescence was obtained from these data with a correlation factor of r=0.82. These investigations indicate that the measurement of culture fluorescence is a practicable tool for monitoring the redox state of a cellular culture, provided the total fluorescence signal is adjusted and the investigations are supported by direct measurements of intracellular levels of reduced dinucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387958
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dynamics of yeast cell states during proliferation and non proliferation periods in a brewing reactor monitored by multidimensional flow cytometry (1997)
    Springer
    Bioprocess engineering 17 (1997), S. 287-293 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Current demands of the brewing industry require that increasing amounts of beer be produced in ever-decreasing times, without prejudicing the quality of the product. The four basic ingredients for brewing beer are malt, hops, water and yeast. Of these four materials, yeast is unique, in that its competent handling can reduce the time taken for the brewing process. For this purpose, it is necessary to have information regarding the metabolic acting as well as the physiological state of an individual cell for which flow cytometry is used. Knowledge of changes in DNA, neutral lipid and 3β-hydroxysterol content of the yeast cells during growth, fermentation and storage enables for a time-saving process control. All of these parameters were conveniently monitored by flow cytometry in conjunction with double fluorescent staining techniques, as shown in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004490050387
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A flow cytometric approach for characterization and differentiation of bacteria during microbial processes (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 93-101 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The analysis of growing or resting bacterial populations by flow cytometry offers several advantages over traditional methods for determining mean-value parameters. This method has been applied here to measure both the distribution of single-cell fluorescence intensity and the light-scatter behaviour of the methylotrophical strains of Methylobacterium rhodesianum MB126 and Methylocystis GB25 as well as Pseudomonas fluorescens and a strain isolated from the soil. The four different bacterial populations were analysed concerning the DNA and the poly-3-hydroxybutyrate (PHB) content. A new cell-preservation method is presented. Optimized staining methods for each strain were developed in detail, in two cases DNA had to be dehybridized before staining with a mixture of mithramycin/ethidium bromide. Nile red is used for detecting PHB. Both stains were excited by an argonion laser at 488 nm; fluorescence emission for mithramycin/ethidium bromide was measured from 520 nm and for Nile red from 600 nm onwards. It is shown that changes in the DNA content and in the forward-light-scattering behaviour of the bacterial strains chosen were measurable. These changes could be related to different cultivation conditions and correlated, in the case of strains that accumulate PHB, with alterations of that biopolymer content. In addition it was found that these methods provide a contribution to the differentiation of mixed bacterial populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170629
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A flow cytometric approach for characterization and differentiation of bacteria during microbial processes (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 93-101 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The analysis of growing or resting bacterial populations by flow cytometry offers several advantages over traditional methods for determining mean-value parameters. This method has been applied here to measure both the distribution of single-cell fluorescence intensity and the light-scatter behaviour of the methylotrophical strains of Methylobacterium rhodesianum MB126 and Methylocystis GB25 as well as Pseudomonas fluorescens and a strain isolated from the soil. The four different bacterial populations were analysed concerning the DNA and the poly-3-hydroxybutyrate (PHB) content. A new cell-preservation method is presented. Optimized staining methods for each strain were developed in detail, in two cases DNA had to be dehybridized before staining with a mixture of mithramycin/ethidium bromide. Nile red is used for detecting PHB. Both stains were excited by an argon-ion laser at 488 nm; fluorescence emission for mithramycin/ethidium bromide was measured from 520 nm and for Nile red from 600 nm onwards. It is shown that changes in the DNA content and in the forward-light-scattering behaviour of the bacterial strains chosen were measurable. These changes could be related to different cultivation conditions and correlated, in the case of strains that accumulate PHB, with alterations of that biopolymer content. In addition it was found that these methods provide a contribution to the differentiation of mixed bacterial populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050376
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Correlation between cellular fluorescence and NAD(P)H levels in Alcaligenes eutrophus JMP 134 (1996)
    Springer
    Bioprocess engineering 14 (1996), S. 57-62 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The culture fluorescence of Alcaligenes eutrophus JMP 134 was determined on-line by an Ingold FluorosensorTM and correlated to the intracellular concentrations of reduced nicotinamide adenine dinucleotide (phosphate). The data were obtained from aerobic cultures of the strain growing chemostatically on phenol, phenol+sodium formate and fructose, as well as from aerobic/anaerobic transitions and substrate pulse experiments. The total culture fluorescence was corrected to take into account the inner filter effect of cells. Upon analysing the intracellular concentration of the dinucleotides using HPLC, it became evident that both NADH and NADPH contribute significantly to the fluorescence signal. A linear relationship between the sum of NAD(P)H and the net culture fluorescence was obtained from these data with a correlation factor of r=0.82. These investigations indicate that the measurement of culture fluorescence is a practicable tool for monitoring the redox state of a cellular culture, provided the total fluorescence signal is adjusted and the investigations are supported by direct measurements of intracellular levels of reduced dinucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387958
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    A modular approach to situation identification of the dynamics of bacterial populations synthesizing poly-β-hydroxybutyrate (1999)
    Springer
    Bioprocess engineering 21 (1999), S. 191-200 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Biotechnological processes involving bacteria are strongly nonlinear. Therefore, both their productivity and the final product quality may be considerably improved by applying appropriate control strategies to modulate behavior of the bacteria during transitional states. This requires advance identification of indicative signals by off-line investigation (i.e. experimental analysis) and on-line monitoring, (i.e. real time evaluation). A modular scheme is presented for doing this, which incorporates an Extended Kalman Filter and a prediction filter. If this is based on a suitable process-feature vector, which must be chosen in advance, the system can provide sufficient information to trigger appropriate feedback signals. Thus, it can provide a key element in modular situation control, allowing continuously periodic process management. In this publication the individual modules involved, and their assembly into an integrated system are described. Potential problems concerning selection of the feature vector, and experimental results are also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009071
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Flow-cytometrisches Monitoring von Populationsverteilungen in biotechnischen Prozessen (1997)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 69 (1997), S. 1225-1225 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330690913
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Staining procedures for flow cytometric monitoring of bacterial populations (1993)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 13 (1993), S. 289-297 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dual or multiple parameter flow cytometric analysis is developing into a powerful method for characterizing microbial populations. The distinguishing of the populations only by assignment of size/shape measurements by scattered ligth renders as not satisfactory.To differentiate between the cells, the employment of a specific fluorescence marker is absolutely necessary. Methods are presented for the flow cytometric determination of DNA and the polymer poly-β-hydroxybutyrate (PHB) content in three different bacterial strains. The measurement of the 3β-hydroxysterol content enables the differentation between yeast and bacterial organisms in mixed microbial populations. Monitoring the ratio of live to dead bacterial cells in soil or water samples, e.g. in pure culture systems, is shown.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370130311
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Dynamic process control versus steady-;state fermentation (1992)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 12 (1992), S. 87-97 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Process control strategies, e.g. the steady-;state fermation, which do not consider the cell-;state distribution of cell populations, cause a decrease of yield coefficient connected to an increase of heat-;production coefficient.Contrary to this, the dynamic process control adapts the carbon-;substrate supply to the different repetitive cell-;population states during fermentation. In order to recognize the different states, the cell population is synchronized. The optimum period ofchanging carbon-;substrate supply was found to be dependent on the cell-;doubling time. Dynamic process control causes a higher yield coefficient as well as a lower heat-;production coefficient compared to the steady-;state fermentation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370120205
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Dynamic processing in biotechnical systems (1984)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 4 (1984), S. 275-278 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The adaptive feeding of nutrients especially of carbon and energy sources according to the demand of cells in different cell states during continuous or semicontinuous cultivation is called dynamic processing.The deduction of dynamic process control concepts is aimed at improving the efficiency of biological substrate conversions into cell mass and other reaction products.A growing number of results allows us to postulate that the principle of dynamic processing should be generally applied in biotechnical processes independent of the type of cells and substrates as well as the nature of products.The basis of the deduction of dynamic process control concepts is the exact knowledge of the dependences of efficiency and rate of product synthesis on cell states during the development of cell populations.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370040309
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