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  • 1
    Electronic Resource
    Electronic Resource
    Effect of individual male variability on cryopreservation of northern pike, Esox lucius L., sperm (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Milt from individual males of northern pike, Esox lucius L., was separately cryopreserved. Concentration of spermatozoa in fresh milt and spermatozoa motility before freezing and after thawing was evaluated. Activity of aspartate aminotransferase (AspAT, E.C. 2.6.1.1.) and acid phosphatase (AcP, E.C. 3.1.2.2.) in fresh and thawed sperm were determined. In comparison with the control group, egg fertilization with cryopreserved milt varied from 6.6% to 96.0%, depending on the donor male. Fertilization success with cryopreserved pooled milt was 71.8%. Freezing and thawing procedure caused loss of proteins from injured spermatozoa, resulting in significantly lower enzymatic activity in spermatozoa. Intensity of enzyme leakage in thawed milt correlated negatively with fertilization success. Concentration of spermatozoa could be a possible accessory quality indication, useful when selecting sperm samples appropriate for cryopreservation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.t01-1-00848.x
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  • 2
    Electronic Resource
    Electronic Resource
    Cryopreservation of the milt of the northern pike (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 46 (1995), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seven published extenders, three thawing media and two thawing temperatures were tested in order to determine their suitability for cryopreservation of northern pike (Esox lucius L.) sperm. Sperm motility during successive steps of cryopreservation was evaluated. Erdahl and Graham's extender with the addition of egg yolk proved to be the most efficient (maximum hatching rate of 74.5%) when semen was thawed in 120 mm NaCl solution warmed up to 30° C. No correlations between motility of sperm (diluted in extenders or diluted in extenders and then activated with thawing solution) and the subsequent hatching success were observed. The relationship between motility of thawed sperm and its fertilization ability was considerable, but correlation was not significant. Spermatozoa frozen in some extenders were frequently motile after thawing but they were not able to fertilize the eggs, this resulted in a poor hatching rate. Depending on the extender, the addition of yolk induced either positive or detrimental effects on fertilization success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1995.tb01604.x
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  • 3
    Electronic Resource
    Electronic Resource
    Temperature requirements for growth and survival of larval vendace, Coregonus albula (L.) (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 38 (1991), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Water temperatures from 15.0 to 20.0° C are recommended as the most suitable for sustained production of larval vendace, as indicated by the developmental rates, the instantaneous rates of growth, mortality, and the net biomass gain of larvae reared at eight constant temperatures from 4.8 to 22.1° C. The recommended temperatures are optimal when the available food and photoperiod are not the limiting factors. Temperatures higher than 22.0° C will cause increased mortality, whereas temperatures lower than 15.0° C, although advisable when food is limited, will retard larval growth and development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1991.tb03088.x
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  • 4
    Electronic Resource
    Electronic Resource
    Duration of eleutheroembryonic phase of Coregonus albula (L.) larvae reared at different constant and variable temperatures (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 22 (1991), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. At different temperatures (4.5, 6.0, 8.6, 10.4, 13.5, 15.9 and 19.0°C), time from hatching to exogenous feeding (DEP50) in vendace, Coregonus albula (L.), larvae decreased from 8 to 2 days when the temperature increased from 4-5 to 19 0°C. Starved larvae at 10.4°C, when offered food at successive days after acclimation to different temperatures, started external feeding on the same day, irrespective of the acclimation temperature.Larvae started exogenous feeding after attaining a particular larval development stage (LDS 1-2), while their age (days after hatching) was of secondary significance. In the vendace population the larvae start exogenous (mixed) feeding at a total length of 9.5 mm, irrespective of their age and rearing temperature. As total length of larvae is closely related to their development stage, the length of fish can be used in a hatchery practice as a parameter indicative of the time of the first feeding of larvae or of their release into the stocked lakes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.1991.tb00491.x
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  • 5
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA variation in two samples of northern pike, Esox lucius L. (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The mitochondrial genotype of northern pike, Esox lucius L., was characterized by restriction analysis performed over the entire mtDNA molecule (33 fish) and over a ∼1.4-kb PCR-amplified segment of a mitochondrial non-coding control region (six fish). Out of eight restriction endonucleases used in the present study of the entire mitochondrial genome, the Nco I and Pst I enzymes produced variant fragment patterns which allowed identification of three mitochondrial genotypes. Low mtDNA diversity was shown by estimates of pairwise nucleotide substitution values (d; mean ± SE = 0.49 ± 0.43%), haplotype diversity (h= 0.16) and the nucleotide diversity index (π= 0.06%). The present authors developed a PCR protocol for mitochondrial DNA non-coding control region to search for more variation. Whereas the PCR assays revealed distinct length variation in this mtDNA segment, restriction analysis performed with three additional restriction enzymes showed no variability which could be attributed to a gain or loss of a particular restriction site. Although the mtDNA analysis proved ineffective in genetic discrimination between the two northern pike samples studied, fish with rare mtDNA genotypes could be used for conducting experimental genome engineering or stocking studies with this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1998.00242.x
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  • 6
    Electronic Resource
    Electronic Resource
    Induced spawning in bream, Abramis brama (L.), using carp and bream pituitary extract and hCG (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Induced spawning in bream, Abramis brama (L), was studied using acetone-dried common carp pituitary (CP) and bream pituitary (BP) with or without the addition of human chorionic gonadotrophin (hCG). The total dose administered to fish was of 5.0 mg kg−1 BP or 4.0 mg kg−1 CP with or without 2000-2200 IU hCG kg−1 for females and 2.5 mg kg−1 BP or 2.0 mg kg−1 CP with or without 1000–1100 IU HCG kg−1 for males.In all male treated groups 100% of spermiation was observed: in females the most effective method was a triple injection with hCG and carp pituitary, resulting in 79% of females ovulated (over 68% of eyed eggs). Biological quality of eggs, expressed as a percentage of eyed eggs, was negatively correlated with time elapsing between resolving (final) injection and ovulation. Spawning success, expressed as a value of Se (spawning effectiveness coefficient), was higher in fish treated with triple injection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.t01-1-00840.x
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  • 7
    Electronic Resource
    Electronic Resource
    Rearing of European grayling, Thymallus thymallus L., larvae using dry and live food (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 17 (1986), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. European grayling, Thymallus thymallus L., larvae, after hatching, were fed solely on a dry food diet and compared to those fed live zooplankton. After 28 days of rearing at temperatures ranging from 15·0°C to 18·3°C (mean, 16·4°C) fish fed the dry diet reached 0·21 g individual wet weight and 31mm in total length; those fed zooplankton reached 0·13g and 27·5 mm respectively. The survival rate of fish fed the dry diet was higher (56·6%) than that of fish fed the live food (40·7%). Results are discussed in the light of the development of the alimentary tract in the early ontogeny of grayling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.1986.tb00114.x
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  • 8
    Electronic Resource
    Electronic Resource
    The effect of individual male potency on fertilization ability of fresh and cryopreserved milt of rainbow trout, Oncorhynchus mykiss (Walbaum) (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The goal of the present study was to examine individual male potency in rainbow trout, Oncorhynchus mykiss (Walbaum), expressed as the fertilization ability of fresh and cryopreserved sperm. One female and four males bearing genetic markers enabling determination of the progeny paternity were chosen as gamete donors. Samples of eggs were inseminated with sperm from separate individuals or with pooled sperm. Genetic examination of the progeny obtained after fertilization of eggs with pooled milt showed differences in male potency. The proportions of offspring sired by four individual males after fertilization of eggs with the fresh milt were similar to those obtained after fertilization with cryopreserved milt (correlation r = 0.95; n = 4; P 〈 0.05). These proportions did not correlate with the proportions of progeny resulting from fertilization of eggs when sperm was not pooled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1998.00197.x
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  • 9
    Electronic Resource
    Electronic Resource
    Some properties of bream Abramis brama L. sperm and its cryopreservation (1999)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 30 (1999), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Concentration and motility of spermatozoa, total protein content and its electrophoretic profile, glucose content, activity of aspartate aminotransferase (AspAT) and acid phosphatase (AcP) were assessed in 18 samples of semen from common bream Abramis brama L. males, which were hormonally stimulated to spermiation. Also, milt pooled from four donors was cryopreserved as pellets in vapours of liquid nitrogen (−80 °C) using four extenders (each with or without the addition of hen egg yolk). Mean spermatozoa concentration was 11.68 × 109 mL−1, and mean spermatozoa motility was about 60%. Protein content in seminal plasma was 2.08 mg mL−1; both PAGE and SDS–PAGE showed considerable heterogeneity of protein fractions. Mean glucose content was over 11 mg%. AspAT and AcP activities were detected in both seminal plasma and spermatozoa extracts. As calculated to 1 × 109 spermatozoa, AcP and AspAT activities were almost sixfold and 46-fold higher in spermatozoa than in seminal plasma respectively. In the best variant, cryopreservation attempts resulted in 66.6% of eyed embryos (compared with control fertilization) obtained after fertilization of eggs with cryopreserved semen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1999.00399.x
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  • 10
    Electronic Resource
    Electronic Resource
    Utilization of body stores in embryonated ova and larvae of two coregonid species (Coregonus lavaretus L. and C. albula L.)
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 79 (1984), S. 329-334 
    Details
    ISSN: 0300-9629
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0300-9629(84)90521-8
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