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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of the milt of the northern pike (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 46 (1995), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seven published extenders, three thawing media and two thawing temperatures were tested in order to determine their suitability for cryopreservation of northern pike (Esox lucius L.) sperm. Sperm motility during successive steps of cryopreservation was evaluated. Erdahl and Graham's extender with the addition of egg yolk proved to be the most efficient (maximum hatching rate of 74.5%) when semen was thawed in 120 mm NaCl solution warmed up to 30° C. No correlations between motility of sperm (diluted in extenders or diluted in extenders and then activated with thawing solution) and the subsequent hatching success were observed. The relationship between motility of thawed sperm and its fertilization ability was considerable, but correlation was not significant. Spermatozoa frozen in some extenders were frequently motile after thawing but they were not able to fertilize the eggs, this resulted in a poor hatching rate. Depending on the extender, the addition of yolk induced either positive or detrimental effects on fertilization success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1995.tb01604.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genotyping of individual fish oocytes based on polymerase chain reaction amplification of mitochondrial DNA (1999)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 30 (1999), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1999.00347.x
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  • 3
    Electronic Resource
    Electronic Resource
    Computer-controlled freezing of rainbow trout Oncorhynchus mykiss (Walbaum) spermatozoa for routine programmes (1999)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 30 (1999), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of extender composition and freezing rate on cryopreservation efficiency of refrigerated spermatozoa of rainbow trout Oncorhynchus mykiss (Walbaum) were evaluated in order to test the suitability of a computer-controlled ultrafreezer to cryopreserve milt samples obtained in field conditions and stored for several hours. A very highly significant first-order interaction between freezing rate and the type of extender was found. Six of the eight experimental variants did not differ significantly, resulting, after fertilization of eggs with cryopreserved sperm, in a range of 62.3–74.8% of eyed embryos. This procedure was effective for samples stored at 1 °C for 2 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1999.00381.x
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  • 4
    Electronic Resource
    Electronic Resource
    Fractional factorial design of screening experiments on cryopreservation of fish sperm (2000)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 31 (2000), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Milt of grass carp Ctenopharyngodon idella (Val.) and goldfish Carassius auratus auratus (L.) was cryopreserved in experiments following &Hf;1 2 and &Hf;1 4 fractional factorial designs. The efficiency of a graphical method and manova was tested, as well as the informative strength of both designs. Fractional factorial design was very suitable for screening experiments, and it allowed separation of important and unimportant effects. A graphical method showed the same efficiency and reliability as manova. The &Hf;1 4 fractional factorial design was less informative than the &Hf;1 2 fractional factorial design, because it did not allow an estimation of the interactions. The application of fractional factorial designs for multifactorial screening experiments on cryopreservation of fish sperm has been implemented in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.2000.00383.x
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of individual male variability on cryopreservation of northern pike, Esox lucius L., sperm (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Milt from individual males of northern pike, Esox lucius L., was separately cryopreserved. Concentration of spermatozoa in fresh milt and spermatozoa motility before freezing and after thawing was evaluated. Activity of aspartate aminotransferase (AspAT, E.C. 2.6.1.1.) and acid phosphatase (AcP, E.C. 3.1.2.2.) in fresh and thawed sperm were determined. In comparison with the control group, egg fertilization with cryopreserved milt varied from 6.6% to 96.0%, depending on the donor male. Fertilization success with cryopreserved pooled milt was 71.8%. Freezing and thawing procedure caused loss of proteins from injured spermatozoa, resulting in significantly lower enzymatic activity in spermatozoa. Intensity of enzyme leakage in thawed milt correlated negatively with fertilization success. Concentration of spermatozoa could be a possible accessory quality indication, useful when selecting sperm samples appropriate for cryopreservation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.t01-1-00848.x
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of individual male potency on fertilization ability of fresh and cryopreserved milt of rainbow trout, Oncorhynchus mykiss (Walbaum) (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The goal of the present study was to examine individual male potency in rainbow trout, Oncorhynchus mykiss (Walbaum), expressed as the fertilization ability of fresh and cryopreserved sperm. One female and four males bearing genetic markers enabling determination of the progeny paternity were chosen as gamete donors. Samples of eggs were inseminated with sperm from separate individuals or with pooled sperm. Genetic examination of the progeny obtained after fertilization of eggs with pooled milt showed differences in male potency. The proportions of offspring sired by four individual males after fertilization of eggs with the fresh milt were similar to those obtained after fertilization with cryopreserved milt (correlation r = 0.95; n = 4; P 〈 0.05). These proportions did not correlate with the proportions of progeny resulting from fertilization of eggs when sperm was not pooled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1998.00197.x
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  • 7
    Electronic Resource
    Electronic Resource
    Some properties of bream Abramis brama L. sperm and its cryopreservation (1999)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 30 (1999), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Concentration and motility of spermatozoa, total protein content and its electrophoretic profile, glucose content, activity of aspartate aminotransferase (AspAT) and acid phosphatase (AcP) were assessed in 18 samples of semen from common bream Abramis brama L. males, which were hormonally stimulated to spermiation. Also, milt pooled from four donors was cryopreserved as pellets in vapours of liquid nitrogen (−80 °C) using four extenders (each with or without the addition of hen egg yolk). Mean spermatozoa concentration was 11.68 × 109 mL−1, and mean spermatozoa motility was about 60%. Protein content in seminal plasma was 2.08 mg mL−1; both PAGE and SDS–PAGE showed considerable heterogeneity of protein fractions. Mean glucose content was over 11 mg%. AspAT and AcP activities were detected in both seminal plasma and spermatozoa extracts. As calculated to 1 × 109 spermatozoa, AcP and AspAT activities were almost sixfold and 46-fold higher in spermatozoa than in seminal plasma respectively. In the best variant, cryopreservation attempts resulted in 66.6% of eyed embryos (compared with control fertilization) obtained after fertilization of eggs with cryopreserved semen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1999.00399.x
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  • 8
    Electronic Resource
    Electronic Resource
    Cryopreservation of sperm of common carp, Cyprinus carpio L. (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Milt of common carp, Cyprinus carpio L. was cryopreserved in pellet form with the use of 12 extenders. Most efficient were: BE2 original extender (containing 85 mM NaCl, 50 mM KCl, 3 mm CaCl2, 1 mm MgCl2 with 10% dimethyl-acetamide (DMA) and 10% addition of hen's egg yolk) and Kurokura et al.'s extender with 15% DMA and 10% yolk (about 73% and 69% of eyed eggs, about 61% and 52% of swim-up larvae, respectively). Within the most effective treatments, survival from the eyed-egg stage to the swim-up stage was similar to that observed in the control group. Survival from the eyed-egg stage to the swim-up stage (percentage of eyed eggs was considered as 100%) was highly significantly and positively correlated with the actual rate of swim-up larvae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.00897.x
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  • 9
    Electronic Resource
    Electronic Resource
    Induced spermiation in 3-year-old sterlet, Acipenser ruthenus L. (2004)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 35 (2004), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Spermiation in 3-year-old sterlet, Acipenser ruthenus (L.), males maintained under warm water conditions was induced by intramuscular injection of either (i) (D-Ala6)-GnRH-ProNHEt (Kobarelin); (ii) mammalian GnRH analogue+metoclopramide (Ovopel); or (iii) human chorionic gonadotropin (Biogonadyl). The volume of milt, sperm concentration and motility were measured. A higher percentage of spermiating males was obtained after Kobarelin or Ovopel treatment (81.8% and 77.7% respectively) in comparison with fish treated with Biogonadyl (40.0%). However, only stimulation with Ovopel guaranteed motile spermatozoa in all spermiating males. Moreover, treatment with Ovopel resulted in the highest average milt volume, sperm concentration and motility. The average total number of motile spermatozoa (milt volume×sperm concentration×sperm motility) per individual was 0.99×109, 5.31×109 and 0.02×109 after stimulation with Kobarelin, Ovopel or Biogonadyl respectively. Our data indicate that Ovopel is a good stimulator of spermation in sturgeons. Moreover, the time of sexual maturation could be significantly reduced in sturgeons maintained in cages under warm water conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.2004.00995.x
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  • 10
    Electronic Resource
    Electronic Resource
    Induced spawning in bream, Abramis brama (L.), using carp and bream pituitary extract and hCG (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Induced spawning in bream, Abramis brama (L), was studied using acetone-dried common carp pituitary (CP) and bream pituitary (BP) with or without the addition of human chorionic gonadotrophin (hCG). The total dose administered to fish was of 5.0 mg kg−1 BP or 4.0 mg kg−1 CP with or without 2000-2200 IU hCG kg−1 for females and 2.5 mg kg−1 BP or 2.0 mg kg−1 CP with or without 1000–1100 IU HCG kg−1 for males.In all male treated groups 100% of spermiation was observed: in females the most effective method was a triple injection with hCG and carp pituitary, resulting in 79% of females ovulated (over 68% of eyed eggs). Biological quality of eggs, expressed as a percentage of eyed eggs, was negatively correlated with time elapsing between resolving (final) injection and ovulation. Spawning success, expressed as a value of Se (spawning effectiveness coefficient), was higher in fish treated with triple injection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.t01-1-00840.x
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