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  • 1
    Electronic Resource
    Electronic Resource
    Growth and vitality in yellow forms of rainbow trout (2000)
    Oxford, UK : Blackwell Science, Ltd
    Journal of applied ichthyology 16 (2000), S. 0 
    Details
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The colour genes controlling the palomino and albino phenotype in the spring spawning rainbow trout [Oncorhynchus mykiss (Walbaum 1792)] strain reared at the Department of Salmonid Research Rutki, Poland were found to have strong detrimental pleiotropic effects on growth and vitality. After 5 and 9 months of rearing, the differences in mean body weights in albino, palomino and wild-coloured trout were statistically highly significant, with albino showing the poorest, palomino as the intermediate and wild-coloured trout having the highest growth. An identical ranking of colour phenotypes was observed for survival. The influence of colour phenotypes on both traits showed a diminishing tendency with increasing age of fish. It is concluded that alleles controlling palomino and albino colouration cannot be recommended as genetic markers. If reference groups are needed, instead of albino trout, palomino trout should be used since their pleiotropic effects are less pronounced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0426.2000.00147.x
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  • 2
    Electronic Resource
    Electronic Resource
    Computer-controlled freezing of rainbow trout Oncorhynchus mykiss (Walbaum) spermatozoa for routine programmes (1999)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 30 (1999), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of extender composition and freezing rate on cryopreservation efficiency of refrigerated spermatozoa of rainbow trout Oncorhynchus mykiss (Walbaum) were evaluated in order to test the suitability of a computer-controlled ultrafreezer to cryopreserve milt samples obtained in field conditions and stored for several hours. A very highly significant first-order interaction between freezing rate and the type of extender was found. Six of the eight experimental variants did not differ significantly, resulting, after fertilization of eggs with cryopreserved sperm, in a range of 62.3–74.8% of eyed embryos. This procedure was effective for samples stored at 1 °C for 2 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1999.00381.x
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success (2000)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 31 (2000), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Collection of fish milt by stripping risks the danger of milt contamination by urine. This may seriously influence milt characteristics and quality, including usefulness for cryopreservation. Urine contamination of milt may be avoided by using a catheter for sperm collection. The objectives of this study were to provide basic characteristics of milt collected with a catheter, to test the usefulness of this milt for cryopreservation, and to correlate characteristics of fresh and cryopreserved semen with sperm fertility rates. Milt from 25 rainbow trout Oncorhynchus mykiss (Walbaum) males were used. All samples were cryopreserved using the pellet method within 1 h of collection, using 0.6 m sucrose and 10% dimethyl sulphoxide (DMSO) as an extender. Catheterization resulted in semen of very good motility (〉 90% motile spermatozoa) and high fertilization rates after cryopreservation (mean fertilization rate 81.8 ± 13.3% of control, at a sperm/egg ratio of 2.4 ± 0.3 × 106). Osmolality of seminal plasma and concentrations of sodium, potassium and magnesium ions had low variability, which suggests that they are important for creating a stable environment for sperm storage in the sperm duct. Higher variability of certain seminal plasma characteristics, such as protein concentration and antiproteinase activity, suggests that these characteristics are related to individual semen features of particular males. A strong correlation of seminal plasma zinc concentration with protein concentration may reflect an importance of zinc in semen biology. Cryopreservation caused a significant release of protein and acid phosphatase from spermatozoa. Our results did not reveal any single characteristic of semen collected by catheter that could be used as a powerful predictor of cryopreservation success, presumably because all samples were of high quality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.2000.00400.x
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of individual male potency on fertilization ability of fresh and cryopreserved milt of rainbow trout, Oncorhynchus mykiss (Walbaum) (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 29 (1998), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The goal of the present study was to examine individual male potency in rainbow trout, Oncorhynchus mykiss (Walbaum), expressed as the fertilization ability of fresh and cryopreserved sperm. One female and four males bearing genetic markers enabling determination of the progeny paternity were chosen as gamete donors. Samples of eggs were inseminated with sperm from separate individuals or with pooled sperm. Genetic examination of the progeny obtained after fertilization of eggs with pooled milt showed differences in male potency. The proportions of offspring sired by four individual males after fertilization of eggs with the fresh milt were similar to those obtained after fertilization with cryopreserved milt (correlation r = 0.95; n = 4; P 〈 0.05). These proportions did not correlate with the proportions of progeny resulting from fertilization of eggs when sperm was not pooled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1998.00197.x
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  • 5
    Electronic Resource
    Electronic Resource
    Failure of interspecies androgenesis in salmonids (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 61 (2002), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Androgenetic development of salmonid embryos was induced in recipient oocytes from the same or other species (intra- or interspecies androgenesis). Parameters for induced androgenesis were investigated in brown trout Salmo trutta and brook trout Salvelinus fontinalis. Reciprocal androgenetic and control crosses were conducted among fishes from three genera: Oncorhynchus (rainbow trout, O. mykiss), Salmo (brown trout) and Salvelinus (brook trout), and within two genera: Salmo (brown trout and Atlantic salmon, S. salar) and Salvelinus (brook trout and Arctic charr, S. alpinus). Live hatched androgenetic progenies were obtained in all intraspecies variants, where oocytes and sperm originated from the same species. Interspecies androgenesis resulted in no viable larvae, despite the fact that most hybrid controls and intraspecies androgenetic individuals were viable. When recipient oocytes originated from other genera (interspecific intergeneric androgenesis), embryonic development ceased in early developmental stages, except for haploid controls of brook trout produced in eggs of brown trout. Survival of embryos to the eyed-egg stage was relatively high in the intrageneric androgenesis experiment. Nevertheless, none of these embryos survived to hatching. Some of the presumed Atlantic salmon individuals developing in brown trout eggs contained maternal DNA, questioning the accuracy of enucleation using irradiation. The inability to induce interspecific androgenesis among the examined salmonid species may have been the result of substantial kariotypical and developmental differences between spermatozoal donors and oocyte recipients, causing an incompatibility between maternal cytoplasmic regulatory factors and the paternal nuclear genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2002.tb01575.x
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