ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
UV and electrochemical detection
;
Particle beam-mass spectrometry
;
Fat-souble vitamin
;
Standard-bore and narrow-bore columns
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The application of different detection techniques; UV, electrochemical (ED) and particle beam-mass spectrometry (PB-MS) for the high-performance liquid chromatography analysis of fat-soluble vitamins were studied. Separation of all compounds examined was performed using both C8 standard-bore and narrow-bore columns for UV and MS detection, whereas in the case of HPLC-ED, a C8 standard-bore column only was used. Detector responses were studied in terms of linear range, detection limit and precision. The HPLC methods with UV, PB-MS and electrochemical detection were applied to the assay of vitamins A and E in an infantformula, powdered-milk sample. Recovery studies showed good results both for vitamins A and E, within-run precision being 2–4% for LC-UV and LC-ED techniques. In addition, excellent aggrement between the results obtained with the detection systems explored was found.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466663
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