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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytical Biochemistry 149 (1985), S. 349-353 
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 969 (1988), S. 166-175 
    ISSN: 0167-4889
    Keywords: (Mouse duodenum) ; Iron absorption ; Subcellular fractionation ; Transferrin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1011 (1989), S. 40-45 
    ISSN: 0167-4889
    Keywords: (Rat hepatocyte) ; Ferritin ; Ferritin receptor ; Iron metabolism
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 53 (1986), S. 391-400 
    ISSN: 1432-0584
    Keywords: Iron absorption ; Transferrin ; In vitro intestinal perfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Isolated non blood-perfused intestinal segments from normal and irondeficient rats were used in vitro. A modification of the luminal perfusion method according to Fisher and Parsons allowed the comparison of iron and transferrin quantities in the serosal fluid at 15 min intervals. Iron transfer in jejunal and ileal segments was directly proportional to the luminal iron concentration within a dose range of 1 to 100 μmol/l, did not show saturation characteristics and was linear over time. Jejunal segments from iron-deficient rats transfered about twice as much iron as the jejunal controls. In ileal segments there was no difference in iron transfer between iron-deficient and control rats; in both cases transfer amounted to approx. 10% of jejunal controls. An exponential correlation was found, when the decreasing transferrin content of the tissue was plotted against the cumulative water transport. Transferrin and albumin release from jejunal and ileal segments into the absorbate cumulated asymptotically, which is typical for wash-out phenomena. As iron transfer cumulated linearly while transferrin release cumulated in an asymptotic manner, the capacity of transferrin to bind iron ions is exceeded roughly 100 times by molar equivalents of iron in the last absorbate fractions. Independence of iron transfer from mucosal transferrin quantities is concluded. As the molar transferrin/albumin ratios do not show significant differences between plasma and the sequence of absorbate samples, a wash-out from the gut's interstitial space is assumed, which makes plasma the most likely origin of transferrin in the mucosa.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0584
    Keywords: Eisen-Resorption ; Eisen-Retention ; Sättigungskurve der Eisen-Resorption ; Magenentleerungszeit und Eisen-Resorption ; Iron absorption ; Iron retention ; Saturation type curve of iron absorption ; Gastric emptying time and iron absorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary 1. The dose dependence of the iron absorption shows a saturation characteristic if iron (0.25–5μmoles Fe/kg body weight) is administered in tied-off intestinal segments of normal and iron-deficient rats. 2. If the iron doses (2.1–570μmoles Fe/kg body weight) are administered by stomach tube only in normal rats a similar dose dependence curve has been obtained as after administration in tied-off intestinal segments. In irondeficient rats the shape of the dose dependence curve is changed and shows no clear-cut saturation characteristic. 3. The differences of these dose dependence curves are discussed with respect to the differences of the methodological conditions. A saturation type kinetic for absorption cannot be expected under all experimental condititions despite of the existence of a transfer system with limited capacity for the transport of iron.
    Notes: Zusammenfassung 1. Die Dosisabhängigkeit der Eisenresorption zeigt Sättigungscharakteristik, wenn Eisen (0,25–5 μMol Fe/kg Körpergewicht) in abgebundene Dünndarmschlingen normaler und eisenarmer Ratten appliziert wird. 2. Wird Eisen (2, l –570 μMol Fe/kg Körpergewicht) dagegen mit der Schlundsonde verabfolgt, so ist nur bei normalen Ratten ein ähnlicher Verlauf der Dosisabhängigkeit zu beobachten wie nach Applikation in abgebundene Dünndarmschlingen. Bei eisenarmen Ratten ist der Verlauf der Dosisabhängigkeit verändert und zeigt keine eindeutige Sättigungscharakteristik. 3. Die Unterschiede im Verlauf der Kurven, die die Eisenresorption in Abhängigkeit von der Dosis beschreiben, werden im Hinblick auf die verschiedenen methodischen Bedingungen diskutiert. Trotz der Tatsache, daß in der Mukosa des Darmes ein Transportsystem für Eisen existiert, ist eine Sättigungskinetik der Resorption nicht unter allen experimentellen Bedingungen zu erwarten.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 51 (1985), S. 41-47 
    ISSN: 1432-0584
    Keywords: Mucosal transferrin ; Iron absorption ; Isolated intestinal cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Mucosal transferrin was determined as transferrin-like immunoreactivity (TLIR) by means of a 2-site immunoradiometric assay (IRMA). Scraped-off mucosal tissue as well as isolated mucosal cells from the duodenum and jejunum of normal and iron-deficient rats before and after a washing procedure were examined. 2. In iron-deficient rats there was about twice as much TLIR in scraped-off mucosal tissue as in the untreated animals. In the duodenum and jejunum of normal and iron-deficient rats, TLIR contents of the isolated cells in the magnitude of 320–510 ng/mg dry weight were found. 3. Washing isolated cells three times in ice-cold Hank's solution resulted in a nearly tenfold decrease of TLIR content in all groups. In contrast the cells' RNA content remained unchanged.
    Type of Medium: Electronic Resource
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