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  • 1
    Electronic Resource
    Electronic Resource
    Phenotyping of malignant hematopoietic cells (1986)
    Springer
    Annals of hematology 52 (1986), S. 99-109 
    Details
    ISSN: 1432-0584
    Keywords: Leukemia ; Lymphoma ; Phenotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1255 cases of leukemia-lymphoma were tested between 1972 and 1984 by multiple marker analysis. Routine leukemia phenotyping was performed using standard morphological and cytochemical techniques in combination with clinical and histo-pathological information; the main emphasis was put on immunological surface marker analysis using erythrocyte rosette assays, TdT and a large panel of poly- and monoclonal antibody tests. The 1255 cases were divided into these major types and subtypes: 349 cases of ALL and related immature T- and Burkitt-lymphomas (cALL, pre B-ALL, B-ALL and Burkitt-lymphomas, T-ALL and immature, mostly leukemic T-lymphomas, Null-ALL), 454 cases of mature T- and B-cell malignancies (T-CLL, mycosis fungoides, Sezary-syndrome, T-lymphomas, B-CLL, hairy cell leukemia, multiple myeloma, B-lymphomas), 263 cases of acute myeloid leukemias (AML, AMMoL/AMoL), 182 cases of chronic myeloid leukemias (CML in chronic phase, CMoL, CML in blast crisis), 6 cases of erythroleukemia and 1 case of megakaryoblastic leukemia. A simplified classification scheme which has been used in our laboratories is presented. Phenotyping is of diagnostic, prognostic and therapeutic relevance, most evidently for patients with ALL. Routine leukemia phenotyping should be performed with highly standardized techniques and reagents and by combining information from several fields in the multiple marker analysis. New areas of leukemia research might become very useful for the routine procedure of phenotyping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00321072
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of theMAGE gene family in human lymphocytic leukemia (1995)
    Springer
    Cancer immunology immunotherapy 41 (1995), S. 95-103 
    Details
    ISSN: 1432-0851
    Keywords: MAGE gene family ; Human lymphocytic leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract TheMAGE gene family, encoding tumor-rejection antigens recognized by cytotoxic T lymphocytes, is frequently expressed in human solid cancers. However, its expression in leukemia has not been well studied. We have investigatedMAGE gene expression at the mRNA level in human leukemia. TheMAGE gene family was expressed in 17 of 34 (50%) examples of T cell leukemia (12/21 patients' peripheral blood mononuclear cells and 5/13 cell lines), in 7 of 16 (44%) cases of B cell leukemia (1/8 and 6/8 respectively), but in none of 23 myelomonocytic leukemia cases (0/16 and 0.7), as evaluated by the primers common to theMAGE-1,-3,-4 (-4a and/or-4b), and-6 genes and the semi-quantificative reverse transcription/polymerase chain reaction method. None of a panel of normal lymphoid cells expressed theMAGE gene family. As revealed by the primers specific for each of theMAGE genes, theMAGE-1,-2,-3,-4 or-6 gene was expressed in 8, 8, 6, 2 or 6 respectively out of 23 types of leukemia cell lines. Expression of the MAGE-1 protein in both the cell lines and patients' cells was confirmed by immunoblot analysis with the polyclonal antibody to recombinant MAGE-1 protein. Cellular MAGE-4 protein in the cell lines was measured by an enzyme-linked immunosorbent assay with the polyclonal and monoclonal antibodies to recombinant MAGE-4b protein. In summary, theMAGE gene family was found to be expressed in the substantial proportion of T cell leukemias, but in no case of myelomonocytic leukemia. Antigens coded by theMAGE gene family could be important molecules for understanding specific immunity against lymphocytic leukemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01527405
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of the MAGE gene family in human lymphocytic leukemia (1995)
    Springer
    Cancer immunology immunotherapy 41 (1995), S. 95-103 
    Details
    ISSN: 1432-0851
    Keywords: Key words MAGE gene family ; Human lymphocytic leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The MAGE gene family, encoding tumor-rejection antigens recognized by cytotoxic T lymphocytes, is frequently expressed in human solid cancers. However, its expression in leukemia has not been well studied. We have investigated MAGE gene expression at the mRNA level in human leukemia. The MAGE gene family was expressed in 17 of 34 (50%) examples of T cell leukemia (12/21 patients’ peripheral blood mononuclear cells and 5/13 cell lines), in 7 of 16 (44%) cases of B cell leukemia (1/8 and 6/8 respectively), but in none of 23 myelomonocytic leukemia cases (0/16 and 0/7), as evaluated by the primers common to the MAGE-1, -3, -4 (-4 a and/or -4 b), and -6 genes and the semi-quantificative reverse transcription/polymerase chain reaction method. None of a panel of normal lymphoid cells expressed the MAGE gene family. As revealed by the primers specific for each of the MAGE genes, the MAGE-1, -2, -3, -4 or -6 gene was expressed in 8, 8, 6, 2, or 6 respectively out of 23 types of leukemia cell lines. Expression of the MAGE-1 protein in both the cell lines and patients’ cells was confirmed by immunoblot analysis with the polyclonal antibody to recombinant MAGE-1 protein. Cellular MAGE-4 protein in the cell lines was measured by an enzyme-linked immunosorbent assay with the polyclonal and monoclonal antibodies to recombinant MAGE-4 b protein. In summary, the MAGE gene family was found to be expressed in the substantial proportion of T cell leukemias, but in no case of myelomonocytic leukemia. Antigens coded by the MAGE gene family could be important molecules for understanding specific immunity against lymphocytic leukemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01527405
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  • 4
    Electronic Resource
    Electronic Resource
    Transverse lesion of spinal cord after accidental exposure to trichloroethylene (1973)
    Springer
    International archives of occupational and environmental health 31 (1973), S. 257-264 
    Details
    ISSN: 1432-1246
    Keywords: Constriction of visual fields ; Estimation of exposure intensity from urinary metabolite levels ; Neurologic damage ; Spinal dermatomes ; Spinal lesion ; Trichloroethylene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case is described in which complete loss of sensation in the trunk and the lower extremities developed after an accidental exposure to an anaesthetic level of trichloroethylene. The clinical picture and negative findings on possible peripheral neuritis suggest a transverse lesion of the spinal cord at the lower cervical to upper thoracic level. Another less affected patient is also described. Estimation of exposure intensity from urinary metabolite levels was attempted and a possible causative agent is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00539030
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  • 5
    Electronic Resource
    Electronic Resource
    The distribution of DR5, MT2, and MB3 specificities on human Ia subsets (1983)
    Springer
    Immunogenetics 17 (1983), S. 371-386 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human Ia molecules were isolated from cells of LG-38, an HLA-homozygous lymphoid cell line of DR5 specificity. Three Ia subsets could be distinguished and separated by using specific alloantisera and a monoclonal antibody with polymorphic reactivity. These subsets carried the specificities DR5 and MT2, MT2 alone and MB3 alone. The structure of the three molecular species was analyzed by microfingerprinting. The subset carrying only MT2 was similar, in both the component α and β chains, to the major subset carrying DR5 and MT2, whereas the subset carrying MB3 was distinct in both chains from the other two subsets. These data are compatible with our previous findings obtained for the products of two Ia loci closely linked to the DR locus and provisionally called DC and BR; they also support the conclusion that the subset carrying only MT2 is an allelic product of the BR locus, whereas the MB3 subset is an allelic product of the DC locus. MT2 appears to be a shared specificity of DR and BR loci products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00372456
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  • 6
    Electronic Resource
    Electronic Resource
    Simultaneous occurrence of myelomonocytic leukemia and multiple myeloma: Involvement of common leukemic progenitors and their developmental abnormality of “lineage infidelity” (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 148 (1991), S. 446-456 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We investigated the origin of leukemic progenitors in a case of the simultaneous occurrence of myelomonocytic leukemia and multiple myeloma (IgG-K). At presentation, myeloperoxidase and nonspecific esterase-positive myelomono-cytic cells had proliferated up to 12.2 x 109/liter in the peripheral blood. Bone marrow cell differentials revealed the coexistence of myelomonocytic cells (30%) and atypical plasmacytoid cells (26%). Myelomonocytic cells in peripheral blood expressed both myeloid antigens (CD11b, CD13, CD14, CD15, CD33) and T/B-lymphoid antigens (CD2, CD4, CD5, CD7, CD10, PCA-1). Bone marrow mononuclear cells (BMMC) could be divided into PCA-1 strongly positive and PCA-1 weakly positive populations, which were considered to represent myeloma cells and myelomonocytic cells, respectively; the former were CD2-positive (CD2+), CD14-, and CD15-, whereas the latter were CD2+, CD14+, and CD15+. Immunohistochemical analysis revealed that, in addition to plasmacy-toid cells, a minority of myelomonocytic cells showed a positive reaction for IgG staining, and production of IgG was observed in the culture supernatant of CD14+ myelomonocytic cells in peripheral blood. Southern blot analysis revealed the presence of two identical rearrangement bands of immunoglobulin heavy chain gene in both BMMC containing myeloma cells and myelomonocytic cells and CD14+ myelomonocytic cells in peripheral blood. In a long-term methylcellulose assay, peripheral blood mononuclear cells produced large compact colonies consisting of macrophages and IgG+ plasmacytoid cells (Mφ/P colonies), while BMMC produced a different type of colonies consisting of CD14+ myelomono-blasts, macrophages, and IgG+ plasma cells (Mb/Mφ/P colonies) in addition to Mφ/P colonies. Recloning experiments showed that primary Mb/Mφ/P colonies gave rise to both secondary Mφ/P and Mb/Mφ/P colonies. These observations strongly suggest that common leukemic progenitors provide both myeloma and myelomonocytic leukemia cells, and the mechanism of “lineage infidelity” is probably involved in the development of their “bilineal” differentiation.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041480317
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