Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Structure and expression of tomato mitochondrial genes coding for tRNACys (GCA), tRNAAsn (GUU) and tRNATyr (GUA): A native tRNACys gene is present in dicot plants but absent in monocot plants (1990)
    Springer
    Current genetics 18 (1990), S. 239-243 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondria ; tRNA genes ; Tomato ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequences of tRNAAsn (GUU) and tRNATyr (GUA) genes from tomato mitochondria and their flanking regions have been determined. The tomato mitochondrial tRNAAsn gene is located 2.1 kb downstream from the tRNACys gene reported previously (Izuchi and Sugita 1989) and shows a nearly complete identity with the corresponding chloroplast gene. The tRNATyr gene, which shows only 73% homology with the corresponding chloroplast gene, has to be considered a “native” mitochondrial tRNA gene and is 535 bp from the “chloroplast-like” tRNAAsn gene on the same strand. Northern hybridization analysis revealed that the three tRNA genes are transcribed in tomato mitochondria. Southern hybridization analysis of tomato, sugar beet, rice and wheat mitochondrial DNAs, with oligonucleotide probes for mitochondrial or chloroplast tRNA genes, demonstrated that the mitochondrial tRNACys gene found in tomato is present in dicot plants but not in monocots. On the other hand, a chloroplast-like tRNACys gene exists in monocot plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318387
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Serotonin innervation patterns differ among the various medullary motoneuronal groups involved in upper airway control (1993)
    Springer
    Experimental brain research 95 (1993), S. 100-110 
    Details
    ISSN: 1432-1106
    Keywords: Serotonin ; Sleep apnea ; Retrofacial nucleus ; Nucleus ambiguus ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this study was to test our hypothesis that the serotoninergic system plays a significant role in airway obstruction during sleep, by focusing on patterns of serotoninergic innervation of the medullary motoneurons involved in upper airway control. We used the combined techniques of retrograde labelling of motoneurons with unconjugated cholera toxin B and immunohistochemistry with antiserum against serotonin (5-HT). The retrograde tracers were injected into posterior cricoarytenoid (PCA), cricothyroid (CT), and genioglossal (GG) muscles of the cat. Motoneurons retrogradely labelled from PCA were identified ipsilateral to the injection site in the caudal part of nucleus ambiguus (NA). Serotonin immunoreactive terminals surrounded their somata and proximal dendrites, suggesting a strong influence of serotonin on the PCA-labelled motoneurons. Motoneurons retrogradely labelled from CT were located ipsilaterally in two distinct groups in the rostral NA and in the retrofacial nucleus (RFN). Selective peripheral nerve section revealed that the CT-labelled motoneurons in the NA had axons in the recurrent laryngeal nerve, whereas the other CT-labelled motoneurons in the RFN were innervated through the superior laryngeal nerve. In the RFN, the pattern of 5-HT innervation in relation to the CT-labelled motoneurons was analogous to that observed with the PCA-labelled motoneurons. In the NA, however, 5-HT terminals made few contacts with the CT-labelled motoneurons, although a dense network of 5-HT terminals was present in the surrounding region. In the GG-labelled motoneuron region of the hypoglossal nucleus, 5-HT terminals were apposed to distal dendrites, not to the soma, indicating less effect of serotonin on GG than on PCA activity. The present results demonstrated that the patterns of 5-HT innervation vary according to the type of motoneurons and their projections to the upper airway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229659
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Effects of caudate nucleus stimulation on substantia nigra cell activity in monkey (1993)
    Springer
    Experimental brain research 95 (1993), S. 457-472 
    Details
    ISSN: 1432-1106
    Keywords: Saccadic eye movement ; Microstimulation ; Caudate nucleus ; Substantia nigra pars reticulata ; Basal ganglia ; Monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study provides evidence that the saccadic signals in the caudate nucleus (caudate) are transmitted to the substantia nigra pars reticulata (SNr). We inserted two microelectrodes into the caudate and SNr of monkeys trained to perform saccade tasks. After identifying the functional characteristics of a SNr neuron recorded, we stimulated the caudate (single pulse, 〈100 μA) to see whether its discharge rate changed. Among 138 SNr cells tested, 60 showed responses to stimulation of the caudate: inhibition only (n=21), inhibition-excitation (n=17), excitation only (n=9), and excitation-inhibition (n=13). The latencies were 9.0–32.5 ms (mean 16.7 ms) for the initial inhibitory responses and 6.5–35.0 ms (mean 16.7 ms) for the initial excitatory responses. Pars compacta cells (n=10) were unresponsive. The effect of caudate stimulation was selective in terms of (1) functional type of SNr cells, (2) location of SNr cells, and (3) stimulation site within the caudate. Functional type of SNr cells: saccadic, visual, expectation-related cells were more responsive than auditory, mouth/hand/ arm movement-related, and reward-related cells. Many of the cells whose functional characteristics were unidentified responded to the caudate stimulation. The preferential effects were seen among the functional subtypes: cells related to memory-guided saccades, not visually guided saccades; cells with conditioned visual responses, not simple visual responses. Location of SNr cells: the stimulus effects were seen preferentially in cells in the central part of the SNr, not in the dorsal part. Stimulus site: stronger effects, whether inhibition or excitation, were obtained when the stimulation was applied to the head-body transitional zone where visuooculomotor cells were clustered. Behaviorally contingent correlation of spike activity was found between the caudate-nigral pair of cells. For example when a SNr cell with memorycontingent saccadic activity was inhibited by the caudate stimulation, a caudate cell at or close to the stimulation site may show memory-contingent saccadic activity with a similar movement field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227139
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Rostrocaudal gradients in the neuronal receptive field complexity in the finger region of the alert monkey's postcentral gyrus (1993)
    Springer
    Experimental brain research 92 (1993), S. 360-368 
    Details
    ISSN: 1432-1106
    Keywords: Postcentral gyrus ; Neuronal submodality ; Receptive field complexity ; Rostrocaudal gradients ; Monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the primate postcentral gyrus, the cytoarchitectonic characteristics gradually shift from those of koniocortex to more homotypical parakoniocortex along its rostrocaudal axis. To find the physiological correlates of these changes we examined a large body of data accumulated during a series of our experiments with alert monkeys. Along the rostrocaudal axis of the postcentral gyrus, we found a gradual and continuous increase in the number of neurons with converging receptive fields and those in which receptive field positions or submodalities were not determined. Deep or skin submodality neurons were dominant in area 3a or 3b respectively. The proportion of skin submodality neurons decreased gradually from area 3b to the more caudal part of the gyrus. The proportion of deep submodality neurons was almost constant from area 3b to area 2 inclusive; they were not the majority in area 2. The data are consistent with the hierarchical scheme, i.e., within the postcentral gyrus sensory information is processed from the primary sensory receiving stage to the more associative, integrative stages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229023
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    A unique sequence located downstream from the rice mitochondrialatp6 may cause male sterility (1994)
    Springer
    Current genetics 25 (1994), S. 52-58 
    Details
    ISSN: 1432-0983
    Keywords: Cytoplasmic male sterility ; Antisense RNA ; RFLP ; Cybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Asymmetric cell-fusion of the japonica cultivar ofOryza sativa (rice) with cytoplasmic-male-sterile (CMS) plants bearing cytoplasm derived from Chinsurah Boro II, resulted in two classes of cytoplasmic hybrids (cybrids), fertile and CMS. Southern-blot analysis of the mitochondrial DNA (mtDNA) indicates recombination events around a number of genes; however, the appearance of the CMS character is tightly correlated to reorganization around theatp6 gene, suggesting recombination downstream from theatp6 gene is involved in CMS. The nucleotide sequence downstream fromatp6 contains a pseudogene which was probably created by recombination of the mitochondrial genome. Sense and antisense transcripts of the downstream region ofatp6 were found in CMS-and restored CMS (fertile)-lines, but not in the normal (fertile) line. In the CMS line, several antisense transcripts of theatp6 gene were also found. However, in the restored line which contains a nuclear-encoded gene,Rf-1, the levels of these transcripts were lower than in the CMS line. These results suggest abnormal transcripts of theatp6 gene produced in the antisense direction may be involved in CMS, and that products of the nuclear-encoded restorer gene may reduce abnormal transcription in this region of the mitochondrial genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00712968
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a cDNA encoding aβ subunit of the mitochondrial ATPase from rice (Oryza sativa) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 171-174 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; rice (Oryza sativa) ; atpB ; nucleotide sequence ; mitochondrial ATP synthase complex ; β subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029164
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 475-480 
    Details
    ISSN: 1432-2242
    Keywords: Electroporation ; Rice ; Co-transformation ; β-Glucuronidase ; Hygromycin phosphotransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a method for reproducibly obtaining transgenic rice at a high frequency (10-4): electroporation with a buffer in which chloride ions are replaced with organic acids. Co-transformation frequencies of the β-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes located on two separate plasmids were higher than 50%. Transgenic rice plants contained multiple copies of introduced genes integrated into their genomes in a complex manner. GUS enzyme activity was not proportional to gene copy number. Introduced HPT genes were detected and expressed in the progeny of transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226748
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 185-194 
    Details
    ISSN: 1617-4623
    Keywords: Conserved open reading frames ; Monocots ; Chloroplast DNA ; Sequence duplication ; Multimer formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The entire chloroplast genome of the monocot rice (Oryza sativa) has been sequenced and comprises 134525 bp. Predicted genes have been identified along with open reading frames (ORFs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (Nicotiana tabacum), and a liverwort (Marchantia polymorpha). The same complement of 30 tRNA and 4 rRNA genes has been conserved between rice and tobacco. Most ORFs extensively conserved betweenN. tabacum andM. polymorpha are also conserved intact in rice. However, several such ORFs are entirely absent in rice, or present only in severely truncated form. Structural changes are also apparent in the genome relative to tobacco. The inverted repeats, characteristic of chloroplast genome structure, have expanded outward to include several genes present only once per genome in tobacco and liverwort and the large single copy region has undergone a series of inversions which predate the divergence of the cereals. A chimeric tRNA pseudogene overlaps an apparent endpoint of the largest inversion, and a model invoking illegitimate recombination between tRNA genes is proposed which accounts simultaneously for the origin of this pseudogene, the large inversion and the creation of repeated sequences near the inversion endpoints.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02464880
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Construction of rice cybrid plants (1989)
    Springer
    Molecular genetics and genomics 215 (1989), S. 501-506 
    Details
    ISSN: 1617-4623
    Keywords: Oryza ; Protoplast fusion ; Cybrids ; Mitochondrial DNA ; Cytoplasmic male sterile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genomes of rice cells were transferred to a fertile rice variety (N8) from a cytoplasmic male sterile variety (CMS) by asymmetric protoplast fusion based on metabolic complementation. Protoplasts derived from CMS were X-irradiated (125 krad) and electrofused with protoplasts which had been treated with iodoacetamide. Metabolic complementation, presumably between nuclear and cytoplasmic compartments, enabled fused protoplasts to form colonies at high efficiency. Restriction digest analysis of mitochondrial DNA (mtDNA) indicated that hybrid cells carried mtDNA derived from both parents. Of the plants regenerated from hybrid calli, 68% carried a diploid chromosome set (2n=24) and the rest of them carried 48 chromosomes. All of them expressed the aryl acylamidase I deficient phenotype encoded by the recessive allele of the fertile N8 parent. These results indicate that the novel somatic hybrid plants regenerated were cybrids, deriving their nucleus from the iodoacetamide treated parent and their mitochondria from both parents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427049
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Dynamics of human cardiorespiratory responses to standing on one leg with eyes closed (1997)
    Springer
    European journal of applied physiology 77 (1997), S. 60-65 
    Details
    ISSN: 1439-6327
    Keywords: Key words Balance test ; Vestibulo-sympathetic reflexes ; Muscle proprioceptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The postural control system has been extensively studied in terms of somatic motor function but little is known about its connection with human autonomic function. The purpose of this study was to determine the cardiorespiratory changes in response to the 1-min balance test that was performed by standing on one leg with eyes closed (SOLEC) or eyes open (SOLEO) in 12 healthy young women [mean age 20.7(SD 3.3) years]. Heart rate (HR), respiratory rate (RR), duration of inspiration and expiration, tidal volume (V T), and oxygen uptake (V˙O2) were measured during the test. The SOLEC test produced rapid increases in HR at the onset. There were significant increases in HR later during the test (P 〈 0.05). Metabolic rate (V˙O2) showed a gradual increase during the SOLEC test, indicating that the late responses could have been partly due to metabolic changes. The RR increased significantly at the onset of the test (P 〈 0.05), and remained elevated until the end of the test. The early responses were considered to be mediated neurally through the postural control system which receives the afferent inputs arising from the vestibular system and from muscle proprioceptors of the leg. In contrast, SOLEO caused small insignificant changes in HR, RR, V T and O2, suggesting that a visual input is essential for balancing a postural change. The SOLEC test may have potential as a test of autonomic function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004210050300
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...