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  • 1
    Electronic Resource
    Electronic Resource
    Orally active cephalosporins and penicillins (1980)
    s.l. : American Chemical Society
    Journal of medicinal chemistry 23 (1980), S. 405-412 
    Details
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jm00178a011
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  • 2
    Electronic Resource
    Electronic Resource
    Parameters controlling yeast hybrid yield in electrofusion: the relevance of pre-incubation and the skewness of the size distributions of both fusion partners
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 899 (1987), S. 25-34 
    Details
    ISSN: 0005-2736
    Keywords: (Yeast) ; Dielectrophoresis ; Electrical breakdown ; Electrofusion ; Particle analyzer ; Protoplast index ; Volume distribution
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(87)90235-5
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  • 3
    Electronic Resource
    Electronic Resource
    Stably inherited killer activity in industrial yeast strains obtained by electrotransformation (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 96 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Killer-sensitive strains of Saccharomyces cerevisiae and Saccharomyces carlsbergensis were transformed by electroinjection using double-stranded RNA isolated from a superkiller strain. Various recipient strains were used: both thermo-resistant and thermo-sensitive as well as mutants of industrial strains. Conversion of respiratory competent (rho+) into respiratory deficient (rho−) strains (mutants) resulted in a significant increase of the yield of electrotransformants and/or of longterm killer stability. Electrotransformation of rho− mutants of distillery and brewery strains resulted in more than 100 clones, which exhibited weak or strong killer activity over some or all of the experimental period of 10 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05400.x
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  • 4
    Electronic Resource
    Electronic Resource
    Large-scale production of yeast hybrids by electrofusion (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 24 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Large-scale production of electrically fused yeast protoplasts from Saccharomyces cerevisiae AH 22[pADH 040-2] and S. cerevisiae AH215 was achieved by the use of the so-called helical fusion chamber. Both strains were of the same mating type a and carried the following auxotrophic markers: his4 in the case of AH22 and leu2, his3 in the case of AH215.AH22 also is a carrier of the plasmid pADH 040-2. This plasmid confers the leu2 gene of yeast and the β-lactamase gene from Escherichia coli, and this feature enables quick detection of plasmid-positive cells.After dielectrophoresis (275 V/cm, 800 kHz) fusion was induced by two field pulses (10 kV/cm, 10 μs duration) applied at an interval of 0.5 s. 50 to 60 hybrids per run were isolated after regeneration on selection medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01249.x
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  • 5
    Electronic Resource
    Electronic Resource
    Transmission of killer activity into laboratory and industrial strains of Saccharomyces cerevisiae by electroinjection (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 70 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The killer character was electrically introduced into protoplasts of three yeast strains. These were the killer-negative variant of the K1 killer strain Saccharomyces cerevisiae T 158 C (his−); the killer-sensitive laboratory strain S. cerevisiae AH 215 (leu−, his−); and the killer-sensitive industrial strain S. cerevisiae AS 4/H2 (rho−). The killer dsRNA used for electroinjection was isolated from the super-killer strain S. cerevisiae T 158 C. Optimum numbers of tranformed cells from obtained after regeneration and selection in appropriate media if the protoplasts were exposed to three exponentially decaying field pulses of 18.2 kV/cm strength and 40 μs duration at 4°C. In the case of the killer-negative variant of S. cerevisiae T 158 C the majority of the protoplasts were transformed, whereas in the case of the two other strains the yield of transformed clones was much less. This latter result is expected if the expression of the electroinjected dsRNA was diminished in these two strains.Gel electrophoresis of the dsRNA of the clones of the three strains supported the conclusion that the transformed clones exhibited killer activity. The transformed clones of all three species were stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb03779.x
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of the composition of the fusion medium on the yield of electrofused yeast hybrids (1985)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 27 (1985), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Electrofusion between cells of yeast strains with different genetic markers in isotonic sorbitol solutions leads to high yields of hybrids when 0.1 mM Ca2+ and 0.5 mM Mg2+ salts are aded. On average, 1000–2000 hybrids are obtained when electrofusion is performed (in a helical chamber) compared to a yield of about 40–120 in the absence of these bivalent cations. A further increase in yield can be achieved by the addition of 1 mg/ml albumin, which results in up to 4000 hybrids per experimental run. The entire fusion process leads to very reproducible results in the presence of these substances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1985.tb00666.x
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  • 7
    Electronic Resource
    Electronic Resource
    Mechanisms of electrostimulated uptake of macromolecules into living cells (1990)
    Springer
    Naturwissenschaften 77 (1990), S. 543-545 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01139269
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  • 8
    Electronic Resource
    Electronic Resource
    Defect filling with demineralized bone matrix. (1998)
    Springer
    Der Orthopäde 27 (1998), S. 80-88 
    Details
    ISSN: 1433-0431
    Keywords: Key words Demineralized bone matrix (DBM) • Bone formation • Fluorescence histology • Hydroxylapatite • Histomorphometry • Mini pigs ; Schlüsselwörter Demineralisierte Bone-Matrix • Knochenneubildung • Fluoreszenzhistologie • Hydroxylapatitkeramik • Histomorphometrie • Minischwein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Das Hauptziel dieser Arbeit war es, in vergleichenden Untersuchungen am Minischwein die Stimulation der Knochenheilung im ersatzstarken Lager durch demineralisierte „bone-matrix-chips“ (DBM) sowie DBM-Gel (Grafton) gegen die knöcherne Integration einer Hydroxylapatitkeramik (Endobon) zu untersuchen. Folgende Ergebnisse konnten erzielt werden: 1. Allogene DBM in Chips- und in Gelform hatte keine osteostimulative Wirkungen auf die Knochenheilung im ersatzstarkem Lager. Nach 12 Wochen war der Defekt bei beiden Applikationsarten der DBM noch nicht knöchern durchbaut. 2. Der Knocheneinwuchs bis zum 35. Tag bei der Pressfit-implantierten HA-Keramik Endobon war nur randständig, während nach 12 Wochen die Keramik vollständig knöchern durchbaut war. Es kam zu einem engen Keramikknochenverbund. 3. Diese Ergebnisse im Rahmen der vorliegenden tierexperimentellen Untersuchung konnten durch eine zeitgerechte Dynamik der Knochenneu- und -umbildungen anhand von histologischen Fluoreszenzübersichten ermittelt werden. Die zeitliche Bestimmung der Knocheneinheilrate wurde durch tägliche Markierung der Tiere mit fluoreszierenden Farbstoffen ermöglicht.
    Notes: Summary The aim of this study was to evaluate the bone stimulation forced by Demineralized Bone Matrix (DBM)-Chips and – Gel in comparison to the bone –ingrowth into a porous hydroxylapatite ceramic (Endobon) in mini pigs. The following results were obtained: 1. DBM-Chips and DBM-Gel did not stimulate bone healing when filled into cancellous bone defects. The defect did not heal within 12 weeks. 2. Up to 35 days the least amount of new bone formation was observed within porous hydroxylapatite ceramic. Up to 12 weeks completly bone ingrowth in to the ceramic has been seen with close bonding between new formed bone and the ceramic trabeculae. 3. By continuous labelling with fluorochromes the new bone formation could be analysed by fluorescence microscopy and the dynamics could be related to time after implantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00003482
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  • 9
    Electronic Resource
    Electronic Resource
    Knorpelknochentransplantation versus autogene Chondrocytentransplantation Eine prospektive vergleichende klinische Studie (2000)
    Springer
    Der Chirurg 71 (2000), S. 1090-1097 
    Details
    ISSN: 1433-0385
    Keywords: Schlüsselwörter: Gelenkknorpeldefekt ; autologe Chondrocytentransplantationen ; autogene osteochondrale Transplantationen ; Knorpelbiologie. ; Keywords: Full-thickness cartilage defect ; Autologous chondrocyte transplantation ; Autologous osteochondral transplantation ; Cartilage biology.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract. Introduction: The treatment of full-thickness cartilage defects still represents a problem that has not yet been solved satisfactorily. Current methods used to cover defects in the knee joint are osteochondral cylinder transplantation (OCT) and autologous chondrocyte transplantation (ACT). Methods: With a prospective clinical investigation, at the time being with 2-year results, we have examined ACT in comparison to OCT in 20 patients with regard to clinical and histomorphological (histology, immunohistochemistry, RES) outcome. Results: We found equally good results with both methods in Lysholm, Meyers and Tegner Activity Scores. Histomorphologic evaluation of biopsies obtained by arthroscopy after ACT showed a defect filling in all cases, mainly with fibrous cartilage, while localized areas of hyalinelike regenerative cartilage were documented near the base.We did not see any histomorphologically visible change in the transplants after OCT. Conclusion: At the time we prefer OCT instead of ACT given the correct indication.
    Notes: Zusammenfassung. Einleitung: Die Behandlung tiefreichender Knorpelschäden stellt nach wie vor ein noch nicht zufriedenstellend gelöstes Problem dar. Aktuelle Methoden zur Defektdeckung am Kniegelenk sind die osteochondrale Zylindertransplantation (OCT) und die autogene Chondrocytentransplantation (ACT). Methoden: Im Rahmen einer prospektiv angelegten klinischen Untersuchung mit derzeit 2 Jahresergebnissen haben wir die ACT im Vergleich zur OCT bei jeweils 20 Patienten hinsichtlich des klinischen und histomorphologischen (Histologie, Immunhistochemie, RES ) Outcome überprüft. Ergebnisse: Hierbei fand sich bei beiden Methoden im Lysholm-, Meyers- sowie dem Tegner-Aktivitäts-Score ein gleich gutes Ergebnis. Die histomorphologische Auswertung der arthroskopisch gewonnenen Biopsierate nach ACT zeigten eine Defektfüllung vornehmlich mit Faserknorpel, wobei basisnah sich begrenzte Areale hyalinähnlicher Knorpelregenerate nachweisen ließen. Eine histomorphologisch erkennbare Veränderung der Transplantate nach OCT sahen wir nicht. Schlußfolgerung: Die OCT wird derzeit von uns der ACT bei korrekter Indikationsstellung vorgezogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001040051184
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  • 10
    Electronic Resource
    Electronic Resource
    Barium-cross-linked alginate beads: a simple, one-step method for successful immunoisolated transplantation of islets of Langerhans (1992)
    Springer
    Acta diabetologica 29 (1992), S. 99-106 
    Details
    ISSN: 1432-5233
    Keywords: Bioartificial pancreas ; Porcine islets ; Rat islets ; Xenogeneic transplantation ; Alginate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Transplantation of xenogeneic islets in immunoisolating membranes may solve the problems of the availability of human donor organs and long-term immunosuppression. Alginates are widely used for microencapsulation of isolated islets. This study presents data of a new method in which alginate was cross-linked with barium ions. In the perifusion experiment microencapsulated rat islets showed a biphasic insulin release with a short delay of the first phase. During static glucose challenge the insulin release ranged from 40% to 70% compared to free floating controls. In 3 of 11 diabetic mice, transplantation of 800 barium-alginate-bead encapsulated rat islets resulted in a non-fasting normoglycaemia at least up to 70 days. In the same model, transplantation of 3000 encapsulated porcine islets resulted in 6 of 10 recipients in normoglycaemia up to day 70, while 3 animals were still normoglycaemic on day 100. On day 21 after transplantation of encapsulated rat and porcine islets and intraperitoneal glucose tolerance test revealed rapid glucose assimilation in both groups. Histological examination demonstrated well-preserved islets at the end of the experiments. Immunohistological B-cell staining revealed the absence of recruitment of β-cells in the recipient's own pancreas. The barium-alginate microencapsulation method represents a simple one-step method for effective immunoisolated transplantation of large-scale islet preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572552
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