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  • 1
    Electronic Resource
    Electronic Resource
    Influence of intermittent long-term stimulation on contractile, histochemical and metabolic properties of fibre populations in fast and slow rabbit muscles (1975)
    Springer
    Pflügers Archiv 361 (1975), S. 1-7 
    Details
    ISSN: 1432-2013
    Keywords: Nerve-muscle interaction ; Contractile parameters ; Energy supplying metabolism ; Enzyme activite pattern ; Transformation of fibre types ; Histochemical fibre typing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Slow (m.soleus) and fast (m.tibialis anterior) muscles of the rabbit were subjected to indirect long-term intermittent stimulation (3 weeks, 8 hrs daily) with a frequency pattern of 10 imp/sec. Whereas no changes were observed in case of the slow muscle, stimulation induced profound changes in the fast tibialis anterior muscle. These consisted in a rearrangement of the enzyme activity pattern of energy-supplying metabolism,e.g. decrease in glycogenolytic and glycolytic enzyme activities and severalfold increase in key enzymes of aerobic endoxidation of substrates in β-oxidation and the citric acid cycle. Concomitant with the increase in aerobic oxidative capacity, there was an increased resistance to fatigue. Histochemical studies revealed a strong increase in mitochondria of all fibres. The bimodal distribution of fibre cross-sectional area in the normal tibialis anterior muscle was changed by stimulation into a more homogeneous population of fibres with a smaller cross-sectional area. Despite a 50% increase in time to peak of isometric twitch contraction no changes were observed in the fibre population with regard to myofibrillar ATPase reaction in quantitative evaluation of whole cross-sections of the muscles. The percentage of fibres histochemically classified as slow amounted to 2.8% and 3.1% in control and stimulated tibialis anterior muscle. Nevertheless the data suggest a transformation of the fibre population under the influence of long-term intermittent stimulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587333
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon (1984)
    Springer
    Molecular genetics and genomics 196 (1984), S. 413-420 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA fragment of the broad host range plasmid RP4 carrying the cis-acting DNA recognition site for conjugative DNA transfer between bacterial cells (Mobsite) was cloned into the kanamycin-neomycin resistance transposon Tn5. Using conventrional transposon mutagenesis techniques the new transposon, called Tn5-Mob, can easily be inserted into the host DNA of gram-negative bacteria. A host replicon carrying Tn5-Mob is then mobilizable into any other gram-negative species if the transfer functions of plasmid RP4 are provided in trans. The potential of Tn5-Mob was demonstrated by mobilizing Rhizobium meliloti plasmids as well as the E. coli chromosome at high frequencies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436188
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The two megaplasmids of Rhizobium meliloti are involved in the effective nodulation of alfalfa (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 356-362 
    Details
    ISSN: 1617-4623
    Keywords: R. meliloti ; Megaplasmids ; Infection mutants ; Extracellular polysaccharides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have shown by physical and genetic means that there are two megaplasmids in all strains of Rhizobium meliloti that we have studied. Megaplasmids from several strains of R. meliloti were mobilized to Agrobacterium tumefaciens and to other Rhizobium strains using the Tn5-Mob system. We were also able to resolve these two megaplasmids in agarose gels for most strains, and to show that only one of them hybridized to nif and nod genes. Transfer of this plasmid, the pSym, to Agrobacterium, R. leguminosarum, and R. trifolii strains conferred on these recipients the ability to nodulate alfalfa ineffectively. The second megaplasmid did not appear to have a direct role in nodule initiation. However, we were able to complement extracellular polysaccharide (EPS-) mutants of R. meliloti by transferring this second megaplasmid into them. Furthermore, Tn5-induced EPS- mutants of R. meliloti 2011, which produced ineffective (Fix-) nodules of abnormal morphology, were shown by hybridization and complementation to carry mutations in this second megaplasmid. This demonstrates that both megaplasmids of R. meliloti are necessary for the effective nodulation of alfalfa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333262
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae (1991)
    Springer
    Molecular genetics and genomics 229 (1991), S. 86-95 
    Details
    ISSN: 1617-4623
    Keywords: Rhizobium meliloti ; Rhizobium leguminosarum bv. viciae ; recA ; Homologous recombination ; Symbiotic nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DNA fragments carrying the recA genes of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae were isolated by complementing a UV-sensitive recA − Escherichia coli strain. Sequence analysis revealed that the coding region of the R. meliloti recA gene consists of 1044 by coding for 348 amino acids whereas the coding region of the R. leguminosarum bv. viciae recA gene has 1053 bp specifying 351 amino acids. The R. meliloti and R. leguminosarum bv. viciae recA genes show 84.8% homology at the DNA sequence level and of 90.1% at the amino acid sequence level. recA − mutant strains of both Rhizobium species were constructed by inserting a gentamicin resistance cassette into the respective recA gene. The resulting recA mutants exhibited an increased sensitivity to UV irradiation, were impaired in their ability to perform homologous recombination and showed a slightly reduced growth rate when compared with the respective wild-type strains. The Rhizobium recA strains did not have altered symbiotic nitrogen fixation capacity. Therefore, they represent ideal candidates for release experiments with impaired strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264217
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    Paper (German National Licenses)
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