ISSN:
1573-7217
Keywords:
breast cancer
;
cell kinetics
;
flow cytometry
;
growth curves
;
nude mice
;
xenografts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01810740
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